Please wait a minute...
Frontiers of Agriculture in China

ISSN 1673-7334

ISSN 1673-744X(Online)

CN 11-5729/S

Front Agric Chin    2011, Vol. 5 Issue (3) : 284-290     DOI: 10.1007/s11703-011-1075-5
Comparative mapping of QTLs for H+ secretion of root in maize (Zea mays L.) and cross phosphorus levels on two growth stages
Junyi CHEN(), Li XU
Institute of Medical Biotechnology in Chongqing/Institute of Chinese Medicine in Chongqing, Chongqing Medical and Pharmaceutical College, Chongqing 401331, China
Download: PDF(132 KB)   HTML
Export: BibTeX | EndNote | Reference Manager | ProCite | RefWorks

H+ is a root secretion that affects P acquisition and P-use efficiency (PUE) under deficient phosphorus in maize. The secretion of H+, difference value of H+ between deficient and normal phosphorus (DH), and relative H+ (RH) as well as the quantitative trait loci (QTLs) associated with these traits were determined for a F2:3 population derived from the cross of two contrasting maize (BoldItalic L.) genotypes, 082 and Ye107. By using composite interval mapping (CIM), a total of 14, 8, and 9 distinct QTLs were identified for H+, DH, and RH, respectively. Most loci of QTLs for traits H+, DH, and RH had different cross environments. It showed that H+ secretion possessed an environment-sensitive and multi-gene nature. The gene × environment interaction was actually reflected by H+ secretion. One region for QTL of trait H+ was detected at the interval of bnlg2228-bnlg100 (bin 1.08) on chromosome 1. Coincident QTLs in the important genomic region reflected the cross phosphorus levels, different cross growth stages, and two different cross environments. The QTL explained 10% to 14% total phenotypic variance of H+. Therefore, the above segment (bnlg2228-bnlg100) (bin 1.08) identified on chromosome 1 may be used in the future for MAS to improve the phosphorus efficiency in maize.

Keywords maize      QTL analysis      H+      difference value of H+      relative H+     
Corresponding Authors: CHEN Junyi,   
Issue Date: 05 September 2011
URL:     OR
TraitsParentsF2∶3 families
Tab.1  Estimates of genetic variance and environment variance among 241 F families from the cross of 082 × Ye107
Tab.2  Phosphorus traits of 082 × Ye107
TreatmentsSource of variationSSDegree of freedomF value
G × E--3.54**
P levelsGenotypes0.03912401.59**
G × E--4.55**
G × E--0.412**
Tab.3  value of ANOVA for effects on H
TreatmentsSource of variationSSDegree of freedomF value
G × E--24.67*
G × E--43.26*
Tab.4  value of ANOVA for effects on DH
TreatmentsSources of variationSSDegree of freedomF value
G × E36.65*
G × E37.85*
Tab.5  value of ANOVA for effects on RH
Tab.6  Analyze of correlation among PUE, H, DH, and RH
NameCQPInterval markersClosest markersBinsLODTR2 (%)ADGADir
Tab.7  QTLs detected for H, DH, and RH with the F families from the cross of 082 × Ye107
1 Ae N, Arihara J, Okada K, Yoshihara T, Johansen C (1990). Phosphorus uptake by pigeon pea and its role in cropping systems of the Indian subcontinent. Science , 248(4954): 477-480
doi: 10.1126/science.248.4954.477 pmid:17815599
2 Agrama H A S, Moussa M E (1996). Mapping QTLs in breeding for drought tolerance in maize (Zea mays L.). Euphytica , 91(1): 89-97
doi: 10.1007/BF00035278
3 Agrama H A S, Zakaria A G, Said F B, Tuinstra M (1999). Identification of quantitative trait loci for N use efficiency in maize. Mol Breed , 5(2): 187-195
doi: 10.1023/A:1009669507144
4 Chen J Y, Cai Y L, Xu L, Wang J G, Zhang W L, Liu Z Z, Peng K, Zhu Z J, Huang Z C, Ai J Z, Tang Q, Deng B H, Yang Z G, Luo J, Sun S L (2010). Identification of quantitative trait loci and epistasis for root characteristics and root exudations in maize (Zea mays L.) under deficient phosphorus. J Chongqing Univ: Eng Ed , 9(2): 105-116
5 Chen J, Xu L, Cai Y, Xu J (2008). QTL mapping of phosphorus efficiency and relative biologic characteristics in maize (Zea mays L.) at two sites. Plant Soil , 313(1-2): 251-266
doi: 10.1007/s11104-008-9698-x
6 Chen J, Xu L, Cai Y, Xu J, Xu J (2009). Identification of QTLs for phosphorus utilization efficiency in maize (Zea mays L.) across P levels. Euphytica , 167(2): 245-252
doi: 10.1007/s10681-009-9883-x
7 Guingo E, Hebert Y (1997). Relationships between mechanical resistance of the maize root system and root morphology, and their genotypic and environmental variation. Maydica , 42: 265-274
8 Guingo E, Hebert Y, Charcosset A (1998). Genetic analysis of root traits in maize. Agronomy , 18(3): 225-235
doi: 10.1051/agro:19980305
9 Hinsinger P (1998). How do plant roots acquire mineral nutrients? Chemical processes involved in the rhizosphere. Adv Agron , 64: 225-265
doi: 10.1016/S0065-2113(08)60506-4
10 Hinsinger P (2001). Bioavailability of soil inorganic P in the rhizosphere as affected by root-induced chemical changes: a review. Plant Soil , 237(2): 173-195
doi: 10.1023/A:1013351617532
11 Hinsinger P, Gilkes R J (1996). Mobilization of phosphate from phosphate rock and alumina-sorbed phosphate by the roots of ryegrass and clover as related to rhizosphere pH. Eur J Soil Sci , 47(4): 533-544
doi: 10.1111/j.1365-2389.1996.tb01853.x
12 Jones D L, Darrah P R (1995). Influx and efflux of organic acids across the soil-root interface of Zea mays L. and its implications in rhizosphere C flow. Plant Soil , 173(1): 103-109
doi: 10.1007/BF00155523
13 Jones E S, Liu C J, Gale M D, Hash C T, Witcombe J R (1998). Mapping quantitative trait loci for downy mildew resistance in pearl millet. Theor Appl Genet , 91(3): 448-456
doi: 10.1007/BF00222972
14 Landi P, Albrecht B, Giuliani M M, Sanguineti M C (1998). Seedling characteristics in hydroponic culture and field performance of maize genotypes with different resistance to root lodging. Maydica , 43: 111-116
15 Landi P, Giuliani M M, DaRFNah L L, Tuberosa R, Conti S, Sanguineti M C (2001). Variability for root and shoot traits in a maize population grown in hydroponics and in the field and their relationships with vertical root pulling resistance. Maydica , 46: 177-182
16 Paterson A H, Lan T H, Reischmann K P, Chang C, Lin Y R, Liu S C, Burow M D, Kowalski S P, Katsar C S, DelMonte T A, Feldmann K A, Schertz K F, Wendel J F (1996). Toward a unified genetic map of higher plants, transcending the monocot-dicot divergence. Nat Genet , 14(4): 380-382
doi: 10.1038/ng1296-380 pmid:8944014
17 Pellet D M, Grunes D L, Kochian L V (1995). Organic acid exudation as an aluminum tolerance mechanism in maize (Zea mays L.). Planta , 196(4): 788-795
doi: 10.1007/BF01106775
18 Pellet D M, Papernik L A, Kochian L V (1996). Multiple aluminum resistance mechanisms in wheat: The roles of root apical phosphate and malate exudation. Plant Physiol , 112(2): 591-597
19 Pratapbhanu S (2002). Phosphorus efficiency of wheat and sugar beet seedlings grown in soils with mainly calcium, or iron and aluminium phosphate. Plant Soil , 246(1): 41-52
doi: 10.1023/A:1021567331637
20 Rogers S O, Rehner S, Bledsoe C (1989). Exaction of DNA from Basidiomycetes for ribosomal DNA hybridization. Can J Bot , 67: 1235-1243
21 Tuberosa RSalvi SSanguineti M CMaccaferri MGiuliani SLandi P(2003). Searching for quantitative trait loci controlling root traits in maize: a critical appraisal. Plant and Soil , 255: 35-54
22 Tuberosa R, Sanguineti M C, Landi P, Giuliani M M, Salvi S, Conti S (2002). Identification of QTLs for root characteristics in maize grown in hydroponics and analysis of their overlap with QTLs for grain yield in the field at two water regimes. Plant Mol Biol , 48: 697-712
doi: 10.1023/A:1014897607670 pmid:11999844
23 Tuberosa R, Sanguineti M C, Landi P, Salvi S, Casarini E, Conti S (1998). RFLP mapping of quantitative trait loci controlling abscisic acid concentration in leaves of drought-stressed maize (Zea mays L.). Theor Appl Genet , 97(5-6): 744-755
doi: 10.1007/s001220050951
24 Vos Hogers R, Bleeker M, Reijans M (1995). AFLP: a new technique for DNA fingerprinting. Nucleic Acids Res , 23(21): 4404-4414
25 Yan X, Liao H, Beebe S E, Blair M W, Lynch J P (2004). QTL mapping of root hair and acid exudation traits and their relationship to phosphorus uptake in common bean. Plant Soil , 265(1-2): 17-29
doi: 10.1007/s11104-005-0693-1
26 Zhu J, Kaeppler S M, Lynch J P (2005). Mapping of QTL controlling root hair length in maize (Zea mays L.) under deficient phosphorus. Plant Soil , 270: 299-310
doi: 10.1007/s11104-004-1697-y
27 Zhu J, Lynch J P (2004). The contribution of lateral rooting to phosphorus acquisition efficiency in maize (Zea mays L.) seedlings. Funct Plant Biol , 31(10): 949-958
doi: 10.1071/FP04046
[1] Yizhi FENG, Bu TAO, Minhao PANG, Yingchao LIU, Jingao DONG. Occurrence of major mycotoxins in maize from Hebei Province, China[J]. Front Agric Chin, 2011, 5(4): 497-503.
[2] Junyi CHEN, Li XU. The candidate QTLs affecting phosphorus absorption efficiency and root weight in maize (Zea mays L.)[J]. Front Agric Chin, 2011, 5(4): 456-462.
[3] Junyi CHEN, Yilin CAI, Li XU, Jiuguang WANG, Wenlong ZHANG, Guoqiang WANG, Delin XU, Tianqing CHEN, Xuegao LU, Haiyan SUN, Aiying HUANG, Ying LIANG, Guoli DAI, Hongni QIN, Zuchun HUANG, Zhaojing ZHU, Zhiguo YANG, Jun XU, Shoufeng KUANG. Identification of QTLs for biomass production in maize (Zea mays L.) under different phosphorus levels at two sites[J]. Front Agric Chin, 2011, 5(2): 152-161.
[4] Kai WEI, Hao ZHANG, Xianfeng XU, Zuxin ZHANG, Hewei DU, Yiqin HUANG, . Evaluation of phenotype and genetic diversity of maize landraces from Hubei Province, Southwest China[J]. Front. Agric. China, 2009, 3(4): 374-382.
[5] Hongzhan Lü, Weili LIANG, Guiyan WANG, David J. CONNOR, Glyn M. RIMMINGTON. A simulation model assisted study on water and nitrogen dynamics and their effects on crop performance in the wheat-maize system: (II) model calibration, evaluation and simulated experimentation[J]. Front Agric Chin, 2009, 3(2): 109-121.
[6] Jie YU, Daiwen CHEN, Bing YU. Protective effects of selenium and vitamin E on rats consuming maize naturally contaminated with mycotoxins[J]. Front Agric Chin, 2009, 3(1): 95-99.
[7] Wenchao ZHEN, Shutong WANG, Chengyin ZHANG, Zhiying MA. Influence of maize straw amendment on soil-borne diseases of winter wheat[J]. Front Agric Chin, 2009, 3(1): 7-12.
[8] Wenying SUN, Yuxing ZHANG, Wenying SUN, Wenquan LE, Hai’e ZHANG. Construction of a genetic linkage map and QTL analysis for some leaf traits in pear (Pyrus L.)[J]. Front Agric Chin, 2009, 3(1): 67-74.
[9] ZHANG Weixing, ZHAO Zhi, BAI Guangxiao, FU Fangjing. Study and evaluation of drought resistance of different genotype maize inbred lines[J]. Front. Agric. China, 2008, 2(4): 428-434.
[10] LÜ Xiangling, LI Xinhai, XIE Chuanxiao, HAO Zhuanfang, JI Hailian, SHI Liyu, ZHANG Shihuang. Comparative QTL mapping of resistance to sugarcane mosaic virus in maize based on bioinformatics[J]. Front. Agric. China, 2008, 2(4): 365-371.
[11] GU Jian, LIU Kun, LI Shaoxiang, TIAN Yuxian, YANG Hexian, YANG Mujun. Study on the culture of cut plants in wheat haploid embryo induction by a wheat × maize cross[J]. Front. Agric. China, 2008, 2(4): 391-395.
[12] WANG Yijun, DENG Dexiang, BIAN Yunlong, XU Mingliang. Maize Mutator transposon[J]. Front. Agric. China, 2008, 2(4): 396-403.
[13] DU Xiong, BIAN Xiuju, YANG Fucun, ZHANG Lifeng, ZHANG Weihong. Effects of plastic-film mulching and nitrogen application on forage-oriented maize in the agriculture-animal husbandry ecotone in North China[J]. Front. Agric. China, 2008, 2(3): 266-273.
[14] BAI Yunfeng, YANG Hongchun, QU Lin, ZHENG Jun, ZHANG Jinpeng, WANG Maoyan, XIE Wan, ZHOU Xiaomei, WANG Guoying. Inverted-repeat transgenic maize plants resistant to sugarcane mosaic virus[J]. Front. Agric. China, 2008, 2(2): 125-130.
[15] SUN Qingquan, DONG Shuting, ZHANG Chunqing, ZHANG Ying, MA Dengchao. Establishment of transgenic acceptor and transformation of gene by particle gun in maize inbred line 18-599 (white)[J]. Front. Agric. China, 2008, 2(1): 37-43.
Full text