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Frontiers of Agriculture in China

ISSN 1673-7334

ISSN 1673-744X(Online)

CN 11-5729/S

Front Agric Chin    2011, Vol. 5 Issue (3) : 361-365     DOI: 10.1007/s11703-011-1109-z
RESEARCH ARTICLE |
mRNA level of PKA-c gene in Setosphaeria turcica with different nutrition sources under metal ion or osmotic stress
Qian WANG, Wei ZHAO, Zhimin HAO, Jingao DONG()
Molecular Plant Pathology Laboratory, Agricultural University of Hebei, Baoding 071001, China
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Abstract  

Intracellular signal transduction pathways including MAPK, Ca2+, and cAMP signal transduction pathways play important roles in regulating growth, development, and pathogenesis of phytopathogenic fungi. Protein kinase A (PKA) is a key enzyme in cAMP signaling pathway. The transcription level of the gene encoding catalytic subunit of PKA in Setosphaeria turcica under different culture conditions was analyzed by the semiquantitative RT-PCR method. The expression level of PKA-c gene was the lowest on the medium containing sucrose and starch as the carbon source, and it was distinctly inhibited by Cu2+, but it was independent of nitrogen source. After the addition of different concentrations of sorbitol, it showed the positive correlation between the inhibition affection and concentrations. However, the highest expression was observed in response to NaCl (0.9 mol/L). This research enriched the biological information resource of filamentous fungi and laid a foundation for the functional analysis about signal transduction pathway in phytopathogenic fungi.

Keywords Setosphaeria turcica      PKA      gene expression      semiquantitative RT-PCR     
Corresponding Authors: DONG Jingao,Email:dongjingao@126.com   
Issue Date: 05 September 2011
URL:  
http://academic.hep.com.cn/fag/EN/10.1007/s11703-011-1109-z     OR     http://academic.hep.com.cn/fag/EN/Y2011/V5/I3/361
Primer namePrimer sequence
Tublin-R5′-TAACAACTGGGCAAAGGGTCA-3′
Tublin-L5′-GGGAACTCCTCACGGATGTTG-3′
B-L5′-CGCATCCAAGGGCTACAACAAGTCTG-3′
B-R5′-CTTGACCGTATGCCTCCGTCTCTTCC-3′
Tab.1  Primers used for semiquantitative RT-PCR
Fig.1  Homology tree of gene sequence of .
Fig.2  gene copy number analysis in the genome of. M is DNA molecular weight marker II, Digoxigenin-labeled; 1-3 are I 2, II 3, and II, respectively.
Fig.3  The expression of gene in on different carbon media. Average±standard error is shown; different lower case letters (a–f) at the top of the bars represent significantly different means (<0.001).
Fig.4  The expression of gene in on different nitrogen media. Average±standard error is shown; different lower case letters (a–b) at the top of the bars represent significantly different means (<0.001).
Fig.5  The expression of gene in under different metal ions. Average±standard error is shown; different lower case letters (a–d) at the top of the bars represent significantly different means (<0.001).
Fig.6  The expression of gene in under different concentrations of sorbitol. Average±standard error is shown; different lower-case letters (a–d) at the top of the bars represent significantly different means (<0.001).
Fig.7  The expression of gene in under different concentrations of NaCl. Average±standard error is shown; different lower case letters (a–e) on top of the bars represent significantly different means (<0.001).
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