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Effects of Co2+ on the erythromycin biosynthesis |
| DU Wen1, CHEN Changhua2 |
| 1.State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China; 2.State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China; College of Bioengineering, East China University of Science and Technology, Shanghai 200237, China; |
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Abstract Erythromycin biosynthesis is a highly complicated process, which involves both primary metabolism and secondary metabolism. The specific activities of the key enzymes related to glucose metabolism such as hexose kinase (HK), glucose-6-phosphate dehydrogenase (6-PDH), phosphofructokinase (PFK), and isocitrate dehydrogenase (ICD), were determined in Saccharopolyspora erythraea. The specific activities of the enzymes involved in secondary metabolism, such as methylmalonyl-coenzyme A mutase (MCM) and methylmalonyl-coenzyme A transcarboxylase (MCT), were detected as well. Some organic acids contained in fermentation broth were also analyzed. The results show that Co2+ is able to increase erythromycin biosynthesis. It maybe due to Co2+ improving the specific activities of methylmalonyl-coenzyme A mutase and methylmalonyl-coenzyme A transcarboxylase. Meanwhile, it also enhances the flux of the glucose metabolism pathway.
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Issue Date: 05 September 2007
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