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High-expression of recombinant human concensus interferon-α by Pichia pastoris |
| HAO Yuyou1, SHI Qiqi1, HE Yun1, ZHUANG Yingping1, WANG Yonghong1, ZHANG Siliang1, CHU Ju1, LIU Zhimin2 |
| 1.State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China; 2.Institute of Bioengineering, Academy of Military Medical Sciences, Beijing 100071, China |
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Abstract The present work focused on the high expression of recombinant human consensus interferon-α (cIFN) by Pichia pastoris. The cycle of glycerol feeding, the strategy of methanol feeding and the optimum pH for protein induction were studied. The optimized strategies were a 4-h glycerol-feeding period, induction pH being kept at 5.0 and methanol concentration being kept under 5 g/L. The maximum dry cell weight, cIFN production and bioactivity obtained were 168, 1.24 g/L and 5.4×107 U/mL, respectively.
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Issue Date: 05 December 2007
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