Please wait a minute...
Frontiers of Chemical Science and Engineering

ISSN 2095-0179

ISSN 2095-0187(Online)

CN 11-5981/TQ

Postal Subscription Code 80-969

2018 Impact Factor: 2.809

Front. Chem. Sci. Eng.    2008, Vol. 2 Issue (4) : 443-446    https://doi.org/10.1007/s11705-008-0065-5
Studies on culture condition of new marine bacterium sp. SY01
LAN Wenjian1, MO Linfeng2, LI Houjin2, CAI Chuanghua3, ZHOU Yipin3, YAO Junhua4
1.School of Pharmaceutical Sciences, Sun Yat-sen University; 2.School of Chemistry and Chemical Engineering, Sun Yat-sen University; 3.South China Sea Institute of Oceanology, The Chinese Academy of Sciences; 4.Instrumental Analysis and Research Center, Sun Yat-sen University;
 Download: PDF(99 KB)   HTML
 Export: BibTeX | EndNote | Reference Manager | ProCite | RefWorks
Abstract New marine bacterium Zooshikella sp. SY01, producer of prodigiosin, was isolated from the seawaters of Sanya Bay. The culture conditions of this bacterium were investigated. Zooshikella sp. SY01 was cultured in 2216E media which contained tryptophan, histidine, lactonic acid, camphor, limonene, casein, diphenyl guanidine, coumarin and 1,3-dinitrobenzene, respectively. After 5 days cultivation, the extracts of different culture broths were detected by direct infusion mass spectroscopy using positive ESI mode. As the results, tryptophan, histidine and casein didn’t show any observable influences on the biosynthesis of prodigiosin. Lactonic acid, camphor, limonene, diphenyl guanidine, coumarin could inhibit the bacterium growth and prodigiosin biosynthesis to a certain extent, slower the culture broth to turn red. However, 1, 3-dinitrobenzene inhibited the bacteria to produce prodigiosin completely. MS data suggested that various metabolites with chemodiversity were produced in different culture media. In particular, a series of high-molecular-weight compounds with high relative abundances were observed in the medium containing limonene. To further optimize the culture condition, more new prodigiosin analogues and lead compounds can be obtained and the goal of “one strain-many compounds” can be achieved.
Issue Date: 05 December 2008
 Cite this article:   
LAN Wenjian,MO Linfeng,YAO Junhua, et al. Studies on culture condition of new marine bacterium sp. SY01 [J]. Front. Chem. Sci. Eng., 2008, 2(4): 443-446.
 URL:  
https://academic.hep.com.cn/fcse/EN/10.1007/s11705-008-0065-5
https://academic.hep.com.cn/fcse/EN/Y2008/V2/I4/443
1 Bode H B, Bethe B, Höfs R, Zeeck A . Bigeffects from small changes: possible ways to explore nature's chemicaldiversity. Chem Bio Chem, 2002, 3(7): 619–627
2 Bitzer J, Grosse T, Wang L Z, Lang S, Beil W, Zeeck A . New aminophenoxazinones from a marine Halomonas sp: fermentation, structure elucidation,and biological activity. Journal of Antibiotics, 2006, 59(2): 86–92
3 Yi H, Chang Y H, Oh H W, Bae K S, Chun J . Zooshikellaganghwensis gen. nov., sp. nov., isolated from tidal flatsediments. International Journal of Systematicand Evolutionary Microbiology, 2003, 53(4): 1013–1018.
doi:10.1099/ijs.0.02521-0
4 Li H J, Lan W J, Cai C H, Zhou Y P, Lin Y C . The bioactive pigments frommarine bacteria Pseudomonas sp.In natural products-essential resources for human survival. Singapore: World Scientific Publishing Company, 2007, 188–194
5 Li H J, Lan W J, Cai C H, Zhou Y P, Lin Y C . Biotransformation of limoneneby marine bacteria. Chinese Journal ofAnalytical Chemistry, 2006, 34(7): 946–950.
doi:10.1016/S1872-2040(06)60046-7
6 Sashidhara K V, Rosaiah J N . Various dereplication strategies using LC-MS for rapid natural productlead identification and drug discovery. Natural Product Communications, 2007, 2(2): 193–202
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed