Uptake and accumulation of multiwalled carbon nanotubes change the morphometric and biochemical characteristics of <italic>Onobrychis arenaria</italic> seedlings

doi:10.1007/s11705-012-1290-5

Front Chem Sci Eng

2012, Vol. 6

Issue (2) : 132-138 https://doi.org/10.1007/s11705-012-1290-5

RESEARCH ARTICLE

Uptake and accumulation of multiwalled carbon nanotubes change the morphometric and biochemical characteristics of Onobrychis arenaria seedlings

Elena SMIRNOVA¹(

), Alexander GUSEV², Olga ZAYTSEVA², Olga SHEINA², Alexey TKACHEV³, Elena KUZNETSOVA⁴, Elena LAZAREVA¹, Galina ONISHCHENKO¹, Alexey FEOFANOV^1,5, Mikhail KIRPICHNIKOV^1,5

1. Biology Faculty, Lomonosov Moscow State University, Moscow 119991, Russia; 2. Derzhavin Tambov State University, Tambov 392000, Russia; 3. Siberian Institute of Plants Physiology and Biochemistry, Siberian Branch, Russian Academy of Sciences, Irkutsk 664033, Russia; 4. NanoTechCenter Ltd., Tambov 392000, Russia; 5. Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow 117997, Russia

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Abstract

We have studied the effect of the engineered nanomaterial Taunit, containing multiwalled carbon nanotubes (MWCNTs), on the growth of Onobrychis arenaria seedlings and investigated whether affected plants uptake and accumulate MWCNTs. We found that 100 μg/mL and 1000 μg/mL of Taunit stimulated the growth of roots and stems, and enhanced the peroxidase activity in these parts of plants. Microscopy studies showed the presence of MWCNTs in the root and leaf tissues of seedlings exposed to Taunit, suggesting that MWCNTs have a capacity to penetrate the cell walls, accumulate in roots and translocate to the leaves. Thus the stimulating effect of MWCNTs on seedlings of O. arenaria may be associated with the primary uptake and accumulation of MWCNTs by plant roots followed by translocation to the other plant tissues.

Keywords multiwalled carbon nanotubes plants electron microscopy

Corresponding Author(s): SMIRNOVA Elena,Email:kinggobi@yandex.ru

Issue Date: 05 June 2012

Cite this article:

Elena SMIRNOVA,Alexander GUSEV,Olga ZAYTSEVA, et al. Uptake and accumulation of multiwalled carbon nanotubes change the morphometric and biochemical characteristics of Onobrychis arenaria seedlings[J]. Front Chem Sci Eng, 2012, 6(2): 132-138.

URL:

https://academic.hep.com.cn/fcse/EN/10.1007/s11705-012-1290-5
https://academic.hep.com.cn/fcse/EN/Y2012/V6/I2/132

Tab.1 Morphometric and biochemical characteristics of seedlings exposed to Taunit

Tab.2 Morphometric parameters of seedlings exposed to low concentrations of Taunit

Fig.1 Estimated activity of horseradish peroxidase in the presence of colloidal water solution of Taunit

Fig.2 Whole mount preparations of root (a, b) and leaf (c, d) of seedling grown in the presence of Taunit

Fig.3 TEM analysis of ultrathin sections of seedlings grown in the presence of Taunit. (a): large agglomerate of Taunit adhered to the root surface. (b): agglomerates of Taunit, containing MWCNTs, in the root tissue. MWCNT piercing the cell wall is shown with arrow, enlarged image inserted at the right bottom. (c): accumulation of MWCNTs in the epidermal cell. Arrows show small agglomerates of Taunit embedded into the cell wall. (d): accumulation of Taunit in the leaf cell

Fig.4 Analysis of CNM containing MWCNTs with SAED. (A): ultrathin section of pure Taunit sample. (B): the area on A, analyzed with SAED. (C): diffraction pattern of the same area. (D): ultrathin section of the plant leaf cell containing the accumulation of Taunit with entangled bundles of MWCNTs. (E): the area on D, analyzed with SAED. (F): diffraction pattern of the same area. (G): ultrathin section of the macrophage with inclusion of Taunit, composed of MWCNT bundles. (H): the area on G, analyzed with SAED. (I): diffraction pattern of the same area

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