Cytochrome P450 monooxygenase specific activity reduction in wheat <i>Triticum aestivum</i> induced by soil roxithromycin stress

doi:10.1007/s11783-015-0813-y

Front. Environ. Sci. Eng.

2016, Vol. 10

Issue (2) : 270-275 https://doi.org/10.1007/s11783-015-0813-y

RESEARCH ARTICLE

Cytochrome P450 monooxygenase specific activity reduction in wheat Triticum aestivum induced by soil roxithromycin stress

Kangxin HE,Qixing ZHOU(

)

MOE Key Laboratory of Pollution Processes and Environmental Criteria/Tianjin Engineering Center of Environmental Diagnosis and Contamination Remediation, College of Environmental Science and Engineering, Nankai University, Tianjin 300071, China

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Abstract

Roxithromycin, as widely used medicine and livestock growth promoter, arouses concern because its occurrence and persistence in soil environments. However, effects of roxithromycin in higher plants are still vague. Accordingly, we hypothesized that roxithromycin-contaminated soil may exhibits ecotoxicological effects in wheat (Triticum aestivum). In this study, effects induced by a gradient concentration of roxithromycin stress (0.01, 0.1, 1, 10, and 100 mg·kg⁻¹) was investigated in a 7-d soil test in T. aestivum. Results indicated that the specific activity of cytochrome P450 (CYP450) monooxygenase was decreased dramatically with the concentration of roxithromycin in soil. The IC₅₀ value was 8.78 mg·kg⁻¹ of roxithromycin. On the contrary, the growth related endpoints (i.e., the germination percentage, the biomass and the height), the content related endpoints (i.e., soluble protein content and CYP450 content), and the superoxide dismutase (SOD) activity failed to reveal the roxithromycin-induced effects. Further analysis revealed that the CYP450 monooxygenase specific activity reduction was enzymatic mechanism mediated, other than oxidative stress induced. We conclude that the soil roxithromycin declined the CYP450 monooxygenase activity in T. aestivum by the inhibition of the enzymatic mechanism. Further efforts can include, but are not limited to, investigation of joint effects induced by combined exposure of roxithromycin and the pesticides and evaluation of the similar effects in other higher plants.

Keywords roxithromycin toxic effect cytochrome P450 monooxygenase soil environment Triticum aestivum biomarker

Corresponding Author(s): Qixing ZHOU

Online First Date: 01 September 2015 Issue Date: 01 February 2016

Cite this article:

Kangxin HE,Qixing ZHOU. Cytochrome P450 monooxygenase specific activity reduction in wheat Triticum aestivum induced by soil roxithromycin stress[J]. Front. Environ. Sci. Eng., 2016, 10(2): 270-275.

URL:

https://academic.hep.com.cn/fese/EN/10.1007/s11783-015-0813-y
https://academic.hep.com.cn/fese/EN/Y2016/V10/I2/270

Tab.1 Basic characteristics of the tested soil

Tab.2 Content of soluble protein and CYP450 and the activity of CYP450 monooxygenase and SOD in wheat (Triticum aestivum) after a 7-day exposure to roxithromycin in soil

Tab.3 Germination percentage, shoot biomass and height of wheat (Triticum aestivum) after a 7-day exposure to roxithromycin in soil

1	Zhou Q X. Ecology of Combined Pollution. Beijing: China Environmental Science Press, 1995 (in Chinese)
2	Yu B, Wang X, Yu S, Li Q, Zhou Q. Effects of roxithromycin on ammonia-oxidizing bacteria and nitrite-oxidizing bacteria in the rhizosphere of wheat. Applied Microbiology and Biotechnology, 2014, 98(1): 263–272 https://doi.org/10.1007/s00253-013-5311-1 pmid: 24150789
3	Schlüsener M P, von Arb M A, Bester K. Elimination of macrolides, tiamulin, and salinomycin during manure storage. Archives of Environmental Contamination and Toxicology, 2006, 51(1): 21–28 https://doi.org/10.1007/s00244-004-0240-8 pmid: 16485173
4	Schlüsener M P, Bester K. Persistence of antibiotics such as macrolides, tiamulin and salinomycin in soil. Environmental Pollution, 2006, 143(3): 565–571 https://doi.org/10.1016/j.envpol.2005.10.049 pmid: 16460854
5	Kumar K, Gupta S, Chander Y, Singh A K. Antibiotic use in agriculture and its impact on the terrestrial environment. Advances in Agronomy, 2005, 87(1): 1–54 https://doi.org/10.1016/S0065-2113(05)87001-4
6	Walsh C. Antibiotics: Actions, Origins, Resistance. 1st ed. Washington, DC: ASM Press, 2003, 145–163
7	Luo Y, Mao D, Rysz M, Zhou Q, Zhang H, Xu L, Alvarez P. Trends in antibiotic resistance genes occurrence in the Haihe River, China. Environmental Science & Technology, 2010, 44(19): 7220–7225 https://doi.org/10.1021/es100233w pmid: 20509603
8	Villa P, Sassella D, Corada M, Bartosek I. Toxicity, uptake, and subcellular distribution in rat hepatocytes of roxithromycin, a new semisynthetic macrolide, and erythromycin base. Antimicrobial Agents and Chemotherapy, 1988, 32(10): 1541–1546 https://doi.org/10.1128/AAC.32.10.1541 pmid: 3190183
9	Chang H R, Pechere J C. Effect of roxithromycin on acute toxoplasmosis in mice. Antimicrobial Agents and Chemotherapy, 1987, 31(7): 1147–1149 https://doi.org/10.1128/AAC.31.7.1147 pmid: 3662475
10	Choi K, Kim Y, Jung J, Kim M H, Kim C S, Kim N H, Park J. Occurrences and ecological risks of roxithromycin, trimethoprim, and chloramphenicol in the Han River, Korea. Environmental Toxicology and Chemistry, 2008, 27(3): 711–719 https://doi.org/10.1897/07-143.1 pmid: 17944547
11	Yin C Y, Luo Y M, Teng Y, Zhang H B, Chen Y S, Zhao Y G. Pollution characteristics and accumulation of antibiotics in typical protected vegetable soils. Environmental Sciences, 2012, 33(8): 2810–2816 pmid: 23213909
12	d<?Pub Caret?>e Liguoro M, Cibin V, Capolongo F, Halling-Sørensen B, Montesissa C. Use of oxytetracycline and tylosin in intensive calf farming: evaluation of transfer to manure and soil. Chemosphere, 2003, 52(1): 203–212 https://doi.org/10.1016/S0045-6535(03)00284-4 pmid: 12729703
13	Tamaoki J, Kadota J, Takizawa H. Clinical implications of the immunomodulatory effects of macrolides. American Journal of Medicine Supplements, 2004, 117(9 Suppl 9A): 5S–11S https://doi.org/10.1016/j.amjmed.2004.07.023 pmid: 15586558
14	Zhou Q X, Kong F X, Zhu L. Introduction to Ecotoxicology. Beijing: Science Press, 2004 (in Chinese)
15	Newhouse K E, Smith W A, Starrett M A, Schaefer T J, Singh B K. Tolerance to imidazolinone herbicides in wheat. Plant Physiology, 1992, 100(2): 882–886 https://doi.org/10.1104/pp.100.2.882 pmid: 16653071
16	Christopher J T, Powles S B, Liljegren D R, Holtum J A. Cross-resistance to herbicides in annual ryegrass (Lolium rigidum) : II. chlorsulfuron resistance involves a wheat-like detoxification system. Plant Physiology, 1991, 95(4): 1036–1043 https://doi.org/10.1104/pp.95.4.1036 pmid: 16668088
17	Hurt R A, Qiu X, Wu L, Roh Y, Palumbo A V, Tiedje J M, Zhou J. Simultaneous recovery of RNA and DNA from soils and sediments. Applied and Environmental Microbiology, 2001, 67(10): 4495–4503 https://doi.org/10.1128/AEM.67.10.4495-4503.2001 pmid: 11571148
18	Masubuchi Y, Horie T. Toxicological significance of mechanism-based inactivation of cytochrome p450 enzymes by drugs. Critical Reviews in Toxicology, 2007, 37(5): 389–412 https://doi.org/10.1080/10408440701215233 pmid: 17612953
19	Murray M. Drug-mediated inactivation of cytochrome P450. Clinical and Experimental Pharmacology & Physiology, 1997, 24(7): 465–470 https://doi.org/10.1111/j.1440-1681.1997.tb01228.x pmid: 9248661
20	Bonjoch N, Tamayo P. Protein Content Quantification by Bradford Method, Handbook of Plant Ecophysiology Techniques. Berlin: Springer, 2011, 283–295
21	Li X X, Song Y F, Yang D L, Zhang W, Song X Y. Cytochorme P450 in wheat as a biomarker for diagnosing pollution in soil. Environmental Chemistry, 2006, 25(3): 284–287 (in Chinese)
22	Xiang W S, Wang X J, Ren T R. Expression of a wheat cytochrome P450 monooxygenase cDNA in yeast catalyzes the metabolism of sulfonylurea herbicides. Pesticide Biochemistry and Physiology, 2006, 85(1): 1–6 https://doi.org/10.1016/j.pestbp.2005.09.001
23	Lin D, Xie X, Zhou Q, Liu Y. Biochemical and genotoxic effect of triclosan on earthworms (Eisenia fetida) using contact and soil tests. Environmental Toxicology, 2012, 27(7): 385–392 https://doi.org/10.1002/tox.20651 pmid: 22707219
24	Chen Y M, Hu X G, Jing S, Zhou Q X. Specific nanotoxicity of graphene oxide during zebrafish embryogenesis. Nanotoxicology, online on February 23, 2015 https://doi.org/10.3109/17435390.2015.1005032
25	Coon M J, Ding X X, Pernecky S J, Vaz A D. Cytochrome P450: progress and predictions. FASEB Journal, 1992, 6(2): 669–673 pmid: 1537454
26	Goksøyr A. A semi-quantitative cytochrome P450IA1 ELISA: a simple method for studying the monooxygenase induction response in environmental monitoring and ecotoxicological testing of fish. Science of the Total Environment, 1991, 101(3): 255–262 https://doi.org/10.1016/0048-9697(91)90038-G pmid: 1754879
27	Xiao R, Wang J, Chen J, Sun L, Chen Y. Effects of arecoline on hepatic cytochrome P450 activity and oxidative stress. Journal of Toxicological Sciences, 2014, 39(4): 609–614 https://doi.org/10.2131/jts.39.609 pmid: 25056785
28	Dupont I, Bodénez P, Berthou F, Simon B, Bardou L G, Lucas D. Cytochrome P-450 2E1 activity and oxidative stress in alcoholic patients. Alcohol and Alcoholism (Oxford, Oxfordshire), 2000, 35(1): 98–103 https://doi.org/10.1093/alcalc/35.1.98 pmid: 10684785
29	Carillon J, Rouanet J M, Cristol J P, Brion R. Superoxide dismutase administration, a potential therapy against oxidative stress related diseases: several routes of supplementation and proposal of an original mechanism of action. Pharmaceutical Research, 2013, 30(11): 2718–2728 https://doi.org/10.1007/s11095-013-1113-5 pmid: 23793992
30	Hu X, Mu L, Kang J, Lu K, Zhou R, Zhou Q. Humic acid acts as a natural antidote of graphene by regulating nanomaterial translocation and metabolic fluxes in vivo. Environmental Science & Technology, 2014, 48(12): 6919–6927 https://doi.org/10.1021/es5012548 pmid: 24857237

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