|
|
The impact of ultrasonic treatment on activity of ammonia-oxidizing bacteria and nitrite-oxidizing bacteria in activated sludge |
Siqi Li1, Min Zheng1,2, Shuang Wu1,3, Yu Xue1, Yanchen Liu1(), Chengwen Wang1, Xia Huang1 |
1. State Key Joint Laboratory of Environment Simulation and Pollution Control, School of Environment, Tsinghua University, Beijing 100084, China 2. Advanced Water Management Centre, The University of Queensland, St. Lucia QLD 4072, Australia 3. China Academy of Urban Planning & Design, Beijing 100084, China |
|
|
Abstract Conditions for ultrasonic treatment to achieve partial nitritation are optimized. Ultrasound reduces metabolic activity and releases intracellular metabolites. Mechanical shearing is essential to inhibit nitrite oxidation. The ultrasonic treatment of sludge has been considered as an effective method to facilitate the partial nitritation of municipal sewage. This study aims to reveal the effects of ultrasound on ammonia-oxidizing bacteria (AOB) and nitrite-oxidizing bacteria (NOB). The impact factors including ultrasonic irradiation time and intensity, sludge concentration, thermal effect and released free radicals were studied. The maximized difference between the changes in AOB and NOB activities were obtained with 10 g mixed liquor suspended solids (MLSS)/L, using 0.9 kJ/mL ultrasonic energy density and 12 h interval time. The increased ultrasonic intensity destroyed the floc structure of activated sludge, increased the microbial death, and decreased the cellular ATP level. Further, the mechanism exploration indicated that the mechanical shearing could be a critical factor in achieving the nitritation with inhibitory effect on nitrite oxidation.
|
Keywords
Ultrasonic treatment
Optimal control
Nitrifying bacteria
Mechanism analysis
|
Corresponding Author(s):
Yanchen Liu
|
Issue Date: 30 October 2019
|
|
1 |
M Bashari, A Eibaid, J Wang, Y Tian, X Xu, Z Jin (2013). Influence of low ultrasound intensity on the degradation of dextran catalyzed by dextranase. Ultrasonics Sonochemistry, 20(1): 155–161
https://doi.org/10.1016/j.ultsonch.2012.06.010
pmid: 22818587
|
2 |
R Blackburne, Z Yuan, Keller J (2008). Demonstration of nitrogen removal via nitrite in a sequencing batch reactor treating domestic wastewater. Water Research, 42(8–9): 2166–2176
|
3 |
Y Cao, M C M van Loosdrecht, G T Daigger (2017). Mainstream partial nitritation-anammox in municipal wastewater treatment: Status, bottlenecks, and further studies. Applied Microbiology and Biotechnology, 101(4): 1365–1383
https://doi.org/10.1007/s00253-016-8058-7
pmid: 28084538
|
4 |
Y Chisti (2003). Sonobioreactors: Using ultrasound for enhanced microbial productivity. Trends in Biotechnology, 21(2): 89–93
https://doi.org/10.1016/S0167-7799(02)00033-1
pmid: 12573858
|
5 |
L Ciccolini, P Taillandier, A M Wilhem, H Delmas, P Strehaiano (1997). Low frequency thermo-ultrasonication of Saccharomyces cerevisiae suspensions: Effect of temperature and of ultrasonic power. Chemical Engineering Journal, 65(2): 145–149
https://doi.org/10.1016/S1385-8947(96)03172-5
|
6 |
D Gao, Y Peng, W M Wu (2010). Kinetic model for biological nitrogen removal using shortcut nitrification-denitrification process in sequencing batch reactor. Environmental Science & Technology, 44(13): 5015–5021
https://doi.org/10.1021/es100514x
pmid: 20540490
|
7 |
S Gao, Y Hemar, M Ashokkumar, S Paturel, G D Lewis (2014a). Inactivation of bacteria and yeast using high-frequency ultrasound treatment. Water Research, 60: 93–104
https://doi.org/10.1016/j.watres.2014.04.038
pmid: 24835956
|
8 |
S Gao, G D Lewis, M Ashokkumar, Y Hemar (2014b). Inactivation of microorganisms by low-frequency high-power ultrasound: 1. Effect of growth phase and capsule properties of the bacteria. Ultrasonics Sonochemistry, 21(1): 446–453
https://doi.org/10.1016/j.ultsonch.2013.06.006
pmid: 23835398
|
9 |
S Guerrero, A López-Malo, S M Alzamora (2001). Effect of ultrasound on the survival of Saccharomyces cerevisiae: Influence of temperature, pH and amplitude. Innovative Food Science & Emerging Technologies, 2(1): 31–39
https://doi.org/10.1016/S1466-8564(01)00020-0
|
10 |
Z Han, Y Miao, J Dong, Z Shen, Y Zhou, S Liu, C Yang (2019). Enhanced nitrogen removal and microbial analysis in partially saturated constructed wetland for treating anaerobically digested swine wastewater. Frontiers of Environmental Science & Engineering, 13(4): 52
https://doi.org/doi.org/10.1007/s11783-019-1133-4
|
11 |
G Huang, S Chen, C Dai, L Sun, W Sun, Y Tang, F Xiong, R He, H Ma (2017). Effects of ultrasound on microbial growth and enzyme activity. Ultrasonics Sonochemistry, 37: 144–149
https://doi.org/10.1016/j.ultsonch.2016.12.018
pmid: 28427617
|
12 |
Y Liu, X Li, X Kang, Y Yuan, M Du (2014). Short chain fatty acids accumulation and microbial community succession during ultrasonic-pretreated sludge anaerobic fermentation process: Effect of alkaline adjustment. International Biodeterioration & Biodegradation, 94: 128–133
https://doi.org/10.1016/j.ibiod.2014.07.004
|
13 |
Y Ma, Y Peng, S Wang, Z Yuan, X Wang (2009). Achieving nitrogen removal via nitrite in a pilot-scale continuous pre-denitrification plant. Water Research, 43(3): 563–572
|
14 |
G Marchesini, L Fasolato, E Novelli, S Balzan, B Contiero, F Montemurro, I Andrighetto, S Segato (2015). Ultrasonic inactivation of microorganisms: A compromise between lethal capacity and sensory quality of milk. Innovative Food Science & Emerging Technologies, 29: 215–221
https://doi.org/10.1016/j.ifset.2015.03.015
|
15 |
K Nickel, U Neis (2007). Ultrasonic disintegration of biosolids for improved biodegradation. Ultrasonics Sonochemistry, 14(4): 450–455
https://doi.org/10.1016/j.ultsonch.2006.10.012
pmid: 17289422
|
16 |
Y Peng, G Zhu (2006). Biological nitrogen removal with nitrification and denitrification via nitrite pathway. Applied Microbiology and Biotechnology, 73(1): 15–26
https://doi.org/10.1007/s00253-006-0534-z
pmid: 17028876
|
17 |
W G Pitt, M O McBride, J K Lunceford, R J Roper, R D Sagers (1994). Ultrasonic enhancement of antibiotic action on gram-negative bacteria. Antimicrobial Agents and Chemotherapy, 38(11): 2577–2582
https://doi.org/10.1128/AAC.38.11.2577
pmid: 7872751
|
18 |
P Piyasena, E Mohareb, R C McKellar (2003). Inactivation of microbes using ultrasound: A review. International Journal of Food Microbiology, 87(3): 207–216
https://doi.org/10.1016/S0168-1605(03)00075-8
pmid: 14527793
|
19 |
X Quan, K Huang, M Li, M Lan, B Li (2018). Nitrogen removal performance of municipal reverse osmosis concentrate with low C/N ratio by membrane-aerated biofilm reactor. Frontiers of Environmental Science & Engineering, 12(6): 5
https://doi.org/doi.org/10.1007/s11783-018-1047-6
|
20 |
J Raso, R Pagán, S Condón, F J Sala (1998). Influence of temperature and pressure on the lethality of ultrasound. Applied and Environmental Microbiology, 64(2): 465–471
pmid: 16349498
|
21 |
E V Rokhina, P Lens, J Virkutyte (2009). Low-frequency ultrasound in biotechnology: State of the art. Trends in Biotechnology, 27(5): 298–306
https://doi.org/10.1016/j.tibtech.2009.02.001
pmid: 19324441
|
22 |
X Song, R Liu, L Chen, B Dong, T Kawagishi (2017). Advantages of intermittently aerated SBR over conventional SBR on nitrogen removal for the treatment of digested piggery wastewater. Frontiers of Environmental Science & Engineering, 11(3): 13
https://doi.org/doi.org/10.1007/s11783-017-0941-7
|
23 |
Y Song, T Hahn, I P Thompson, T J Mason, G M Preston, G Li, L Paniwnyk, W E Huang (2007). Ultrasound-mediated DNA transfer for bacteria. Nucleic Acids Research, 35(19): e129
https://doi.org/10.1093/nar/gkm710
pmid: 17890732
|
24 |
P B Subhedar, P R Gogate (2014). Enhancing the activity of cellulase enzyme using ultrasonic irradiations. Journal of Molecular Catalysis. B, Enzymatic: 101: 108–114
https://doi.org/10.1016/j.molcatb.2014.01.002
|
25 |
O Turk, D S Mavinic (1986). Preliminary assessment of a shortcut in nitrogen removal from wastewater. Canadian Journal of Civil Engineering, 13(6): 600–605
https://doi.org/10.1139/l86-094
|
26 |
M Wang, R Li, Q Zhao (2019). Distribution and removal of antibiotic resistance genes during anaerobic sludge digestion with alkaline, thermal hydrolysis and ultrasonic pretreatments. Frontiers of Environmental Science & Engineering, 13(3): 43
https://doi.org/doi.org/10.1007/s11783-019-1127-2
|
27 |
Q Wang, M Kuninobu, K Kakimoto, H I-Ogawa, Y Kato (1999). Upgrading of anaerobic digestion of waste activated sludge by ultrasonic pretreatment. Bioresource Technology, 68(3): 309–313
https://doi.org/10.1016/S0960-8524(98)00155-2
|
28 |
S Wu, M Zheng, Q Dong, Y Liu, C Wang (2018). Evaluating the excess sludge reduction in activated sludge system with ultrasonic treatment. Water Science & Technology, 77(9–10): 2341–2347
https://doi.org/10.2166/wst.2018.164
pmid: 29757186
|
29 |
Q Yang, X Liu, C Peng, S Wang, H Sun, Y Peng (2009). N2O production during nitrogen removal via nitrite from domestic wastewater: Main sources and control method. Environmental Science & Technology, 43(24): 9400–9406
https://doi.org/10.1021/es9019113
pmid: 20000535
|
30 |
M Zheng, H Duan, Q Dong, B J Ni, S Hu, Y Liu, X Huang, Z Yuan (2019a). Effects of ultrasonic treatment on the ammonia-oxidizing bacterial (AOB) growth kinetics. Science of the Total Environment, 690: 629–635
https://doi.org/10.1016/j.scitotenv.2019.06.435
pmid: 31301503
|
31 |
M Zheng, Y C Liu, J Xin, H Zuo, C W Wang, W M Wu (2016). Ultrasonic treatment enhanced ammonia-oxidizing bacterial (AOB) activity for nitritation process. Environmental Science & Technology, 50(2): 864–871
https://doi.org/10.1021/acs.est.5b04178
pmid: 26678011
|
32 |
M Zheng, Y C Liu, K N Xu, C W Wang, H He, W Zhu, Q Dong (2013). Use of low frequency and density ultrasound to stimulate partial nitrification and simultaneous nitrification and denitrification. Bioresource Technology, 146: 537–542
https://doi.org/10.1016/j.biortech.2013.07.044
pmid: 23973972
|
33 |
M Zheng, S Wu, Q Dong, X Huang, Z Yuan, Y Liu (2019b). Achieving mainstream nitrogen removal via the nitrite pathway from real municipal wastewater using intermittent ultrasonic treatment. Ultrasonics Sonochemistry, 51: 406–411
https://doi.org/10.1016/j.ultsonch.2018.07.033
pmid: 30249372
|
|
Viewed |
|
|
|
Full text
|
|
|
|
|
Abstract
|
|
|
|
|
Cited |
|
|
|
|
|
Shared |
|
|
|
|
|
Discussed |
|
|
|
|