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Frontiers of Medicine

ISSN 2095-0217

ISSN 2095-0225(Online)

CN 11-5983/R

Postal Subscription Code 80-967

2018 Impact Factor: 1.847

Front. Med.    2007, Vol. 1 Issue (1) : 49-53     DOI: 10.1007/s11684-007-0010-x
Construction of 6HRE-GFAP-Baxα system specific for glioma gene therapy
TIAN Yongji1, LI Guilin2, GAO Jun2, WANG Renzhi2, KONG Yanguo2, ZHANG Zhenxing2, LI Shifang2, TIAN Shiqiang2, DOU Wanchen2, ZHANG Bo2
1.Department of Neurosurgery, Beijing Tiantan Hospital affiliated China Capital Medical University, Beijing 100050, China; 2.Department of Neurosurgery, Peking Union Medical College Hospital, Beijing 100730, China
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Abstract  The aim of this paper is to construct a specific and high-performance gene therapy system for glioma. We constructed a combined promoter 6HRE-GFAP (Hypoxia Responsive Element, Glial Fibrillary Acidic Protein) by gene recombination techniques according to the hypoxia microenvironment in glioma and tested its efficacy and specificity in cultured cells, and then constructed GFAP-Baxα gene expressing system and determined its promoting of apoptosis in glioma cells. Our primary results showed that in U251 and BT325 cell lines, the activity of GFAP promoter was 16.40 and 4.73-fold of the promoter of hTERT (Human Telomerase Reverse Transcriptase), respectively. The activities of 6HRE-GFAP-promoter increased by 3.08 and 1.30-fold under 2% O2 condition compared with those under 18% O2 condition, while under 0.2% O2 condition increased by 8.90 and 2.69-fold, respectively. The glioma cells showed typical apoptotic signs 90 hours after the transient transfection of GFAP-Baxα. In these primary experiments, it showed that 6HRE-GFAPBaxα system could promote glioma cell apoptosis. It was specific and effective for glioma gene therapy.
Issue Date: 05 March 2007
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