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Frontiers of Medicine

ISSN 2095-0217

ISSN 2095-0225(Online)

CN 11-5983/R

Postal Subscription Code 80-967

2018 Impact Factor: 1.847

Front. Med.    2008, Vol. 2 Issue (1) : 70-74     DOI: 10.1007/s11684-008-0013-2
Protein aggregation in association with delayed neuronal death in rat model of brain ischemia
GE Pengfei1, FU Shuanglin1, LI Wenchen1, WANG Chonghao1, ZHOU Chuibing1, LUO Yinan1, LUO Tianfei2
1.Department of Neurosurgery, First Hospital of Jilin University; 2.Norman Bethune Medicine School, Jilin University
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Abstract  To investigate the relationship between protein aggregation and delayed neuronal death, we adopted rat models of 20 min ischemia. Brain ischemia was produced using the 2-vessel occlusion (2VO) model in rats Light microscopy, transmission electronic microscopy and Western blot analysis were performed for morphological analysis of neurons, and protein detection. The results showed delayed neuronal death took place at 72 h after ischemia-reperfusion, protein aggregates formed at 4 h after reperfusion and reached the peak at 24 h after reperfusion, and Western blot analysis was consistent with transmission electronic microscopy. We conclude that protein aggregation is one of the important factors leading to delayed neuronal death.
Issue Date: 05 March 2008
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