Please wait a minute...
Frontiers of Medicine

ISSN 2095-0217

ISSN 2095-0225(Online)

CN 11-5983/R

Postal Subscription Code 80-967

2018 Impact Factor: 1.847

Front. Med.    2008, Vol. 2 Issue (1) : 113-116     DOI: 10.1007/s11684-008-0021-2
|
Characterization of Bacillus amyloliquefacien contaminating 75% alcohol disinfectant
ZHANG Wanming1, YUAN Yuesha2, BIAN Cangli3, TU Xianyu3, ZHANG Wen4, HUANG Huqiang4, WANG Lan5
1.Department of Pediatrics, Medical School of Yangtze University; 2.Department of Pediatrics, Medical School of Yangtze University; The Laboratory of Molecular Medicine, Medical School of Yangtze University; 3.The Laboratory of Molecular Medicine, Medical School of Yangtze University; 4.Yangtze University Graduate School; 5.Department of Infection Control, the Second Hospital of Jingzhou
Download: PDF(75 KB)   HTML
Export: BibTeX | EndNote | Reference Manager | ProCite | RefWorks
Abstract  The clinical characterization of Bacillus amyloliquefacien contaminating 75% alcohol disinfectants were studied. The bacteria were cultured and observed by using bacterial examination under the hospital infection monitor. According to the regulations, the resistance of bacterial to physical and chemical factors was tested. Drug sensitivity tests for 20 commonly used medicines were carried out using a K-B method. The bacterial plasmids were analyzed using the Birnboim method. The bacteria were found after being cultured in the clinically-used 75% alcohol disinfectant fluid. Their total number was more than 800 cfu/mL and they were identified as Bacillus amyloliquefacien. The bacteria were also found to be resistant to boiling for 5 min. It grew well in 95% alcohol disinfectant and was insensitive to 84 disinfectant fluids containing chlorine (1000 mg/L) and such disinfectants as ozone. They were able to be sterilized better through routine ultraviolet exposure for 30 min or gas pressure. The bacteria contained a 2.5 kb plasmid and were sensitive to 13 drugs and insensitive to 7 drugs of the 20 drugs tested. It was suggested that alcohol disinfectant fluid was easily contaminated by Bacillus amyloliquefacien, and the bacteria was resistant to disinfectant fluids such as alcohol and 84 disinfectants
Issue Date: 05 March 2008
URL:  
http://academic.hep.com.cn/fmd/EN/10.1007/s11684-008-0021-2     OR     http://academic.hep.com.cn/fmd/EN/Y2008/V2/I1/113
1 Pannifer A D Wong T Y Schwarzenbacher R Renatus M Petosa C Bienkowska J Lacy D B Collier R J Park S Leppla S H Hanna P Liddington R C Crystalstructure of the anthrax lethal factorNature 2001 414(6860)160161
2 Mahadtanapuk S Sanguansermsri M Cutler R W Sardsud V Anuntalabhochai S Control of anthracnose causedby colletotrichum musae on curcuma alismatifolia gagnep. Using antagonisticbacillus spp.Am J Agril Biol Sci 2007 2(2)5461
3 Matarante A Baruzzi F Cocconcelli P S Morea M Genotyping andtoxigenic potential of bacillus subtilis and bacillus pumilus strainsoccurring in industrial and artisanal cured sausagesAppl Environ Microbiol 2004 70(9)51685176
4 Nicholson W L Munakata N Horneck G Melosh H J Setlow P Resistance of bacillus endospores to extremeterrestrial and extraterrestrial environmentsMicrobio Mol Biol Rev 2000 64(3)548572
5 Airaksinen U Penttilä T Wahlström E Vuola J M Puolakkainen M Sarvas M Production of chlamydia pneumoniae proteins in bacillussubtilis and their use in characterizing immune responses in the experimentalinfection modelClin Diagn Lab Immunol 2003 10(3)367375
6 Zhang Z R ClinicalMicrobiology and Examination of Microorganisms. Beijing: People'sMedcial Publishing House 2002 499503(in Chinese)
7 Weber D J Saviteer S M Rutala W A Thomann C A In vitro susceptibilityof bacillus spp. to selected antimicrobial agents.Antimicrob Agents Chemother 1988 32(5)642645
8 Wu S X Shen L Plasmid analysis and phagetyping in the study of staphylococcal colonization and disease innewborn infantsZhonghua Yi Xue Za Zhi 1993 8157161(in Chinese)
9 Zhang W M Wu S X Liu G X Study on amotile bacteria of positive blood culture innewborn: the analysis of plasmid and restriction enzyme and determinationof outer membrane proteinShiyong Erke LinchuangZazhi 1994 9(5)257259 (in Chinese)
10 Zhang W M Bian C L Tu X Y Wang L Report on examiningalcohol disinfectant fluid contamined by Bacillus amyloliquefacienZhongguo Xiaoduxue Zazhi 2006 23(6)579580 (in Chinese)
11 Citron D M Appleman M D In vitro activities of daptomycin,ciprofloxacin, and other antimicrobial agents against the cells andspores of clinical isolates of bacillus speciesJ Clin Microbiol 2006 44(10)38143818
12 Logan N A Bacillusspecies of medical and veterinary importanceJ Med Microbiol 1988 25(3)157165
13 Esel D Doganay M Sumerkan B Antimicrobial susceptibilities of 40 isolates of bacillusanthracis isolated in turkeyInt J AntimicrobAgents 2003 22(1)7072
14 Zhu M H Bai P Wang J F Investigation into the Cause of Bacteria ContaminatingDisinfectant Fluid in Use and its ControlZhongguo Xiaoduxue Zazhi 2005 22(3)315 (in Chinese)
15 Gao D Q Synergismof alcohol in disinfectionZhongguo XiaoduxueZazhi 1993 10(2)101 (in Chinese)
16 Zhao G Z Wang X Yang J Observation of the effect of sterilization education onthe contamination of the bottles full of iodine and alcoholHulixue Zazhi 2005 20(5)63 (in Chinese)
No related articles found!
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed