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Frontiers of Medicine

ISSN 2095-0217

ISSN 2095-0225(Online)

CN 11-5983/R

Postal Subscription Code 80-967

2018 Impact Factor: 1.847

Front Med Chin    2009, Vol. 3 Issue (2) : 216-219     DOI: 10.1007/s11684-009-0028-3
RESEARCH ARTICLE |
Expression of inducible nitric oxide synthase in trophoblasts and deciduas in early medical abortion
Geqing XIA, Chaoying WU()
Department of Obstetrics & Gynecology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China
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Abstract  

The purpose of this study was to investigate the expression of inducible nitric oxide synthase in trophoblasts and deciduas in early medical abortion, and study the relationship of medical abortion through mifepristone and inducible nitric oxide synthase (iNOS) in early pregnancy. Expression of iNOS in trophoblasts and deciduas was detected by both in situ hybridization and immunohistochemical assay in 40 patients (experimental group); the positive expression of iNOS was represented by number density (N/S) and positive unit (Pu) using computer color magic image analysis system (CMIAS). All results were compared with that obtained from vacuum aspiration. In the experimental group, N/S and Pu in trophoblasts were 0.120 ± 0.010 and 15.3 ± 2.6, respectively, while in the control group, they were 0.021 ± 0.003 and 3.1 ± 0.5, respectively, and there were significant differences between the two groups. By immunohistochemical assay, N/S and Pu were 0.090 ± 0.010, 10.24 ± 1.55 vs 0.016 ± 0.002, 1.26 ± 0.33 in the trophoblasts of the two groups; there were also significant differences between the two groups. There were lower iNOS expressions in deciduas by in situ hybridization and immunohistochemical assay, and the difference between the two groups was not significant.It was concluded that mifepristone induced medical abortion through the expression of iNOS in trophoblasts but not in deciduas.

Keywords mifepristone      inducible nitric oxide synthase      early pregnancy      placental immunity     
Corresponding Authors: WU Chaoying,Email:cyw287@yahoo.com.cn   
Issue Date: 05 June 2009
URL:  
http://academic.hep.com.cn/fmd/EN/10.1007/s11684-009-0028-3     OR     http://academic.hep.com.cn/fmd/EN/Y2009/V3/I2/216
groupnumbershybridization in situimmunohistochemistry
N/SPuN/SPu
experimental group400.120 ± 0.01015.3 ± 2.60.090 ± 0.01010.24 ± 1.55
control group400.022 ± 0.0033.1 ± 0.50.016 ± 0.0021.26 ± 0.33
P value< 0.05< 0.05< 0.05< 0.05
Tab.1  The expression of iNOS in villi
groupnumbershybridization in situimmunohistochemistry
N/SPuN/SPu
experimental group400.003 ± 0.0010.28 ± 0.040.004 ± 0.0010.24 ± 0.05
control group400.003 ± 0.0030.3 ± 0.040.005 ± 0.0020.25 ± 0.03
P value> 0.05> 0.05> 0.05> 0.05
Tab.2  The expression of iNOS in deciduas
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