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Frontiers of Medicine

ISSN 2095-0217

ISSN 2095-0225(Online)

CN 11-5983/R

Postal Subscription Code 80-967

2018 Impact Factor: 1.847

Front Med Chin    2009, Vol. 3 Issue (2) : 216-219     DOI: 10.1007/s11684-009-0028-3
Expression of inducible nitric oxide synthase in trophoblasts and deciduas in early medical abortion
Geqing XIA, Chaoying WU()
Department of Obstetrics & Gynecology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China
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The purpose of this study was to investigate the expression of inducible nitric oxide synthase in trophoblasts and deciduas in early medical abortion, and study the relationship of medical abortion through mifepristone and inducible nitric oxide synthase (iNOS) in early pregnancy. Expression of iNOS in trophoblasts and deciduas was detected by both in situ hybridization and immunohistochemical assay in 40 patients (experimental group); the positive expression of iNOS was represented by number density (N/S) and positive unit (Pu) using computer color magic image analysis system (CMIAS). All results were compared with that obtained from vacuum aspiration. In the experimental group, N/S and Pu in trophoblasts were 0.120 ± 0.010 and 15.3 ± 2.6, respectively, while in the control group, they were 0.021 ± 0.003 and 3.1 ± 0.5, respectively, and there were significant differences between the two groups. By immunohistochemical assay, N/S and Pu were 0.090 ± 0.010, 10.24 ± 1.55 vs 0.016 ± 0.002, 1.26 ± 0.33 in the trophoblasts of the two groups; there were also significant differences between the two groups. There were lower iNOS expressions in deciduas by in situ hybridization and immunohistochemical assay, and the difference between the two groups was not significant.It was concluded that mifepristone induced medical abortion through the expression of iNOS in trophoblasts but not in deciduas.

Keywords mifepristone      inducible nitric oxide synthase      early pregnancy      placental immunity     
Corresponding Authors: WU Chaoying,   
Issue Date: 05 June 2009
URL:     OR
groupnumbershybridization in situimmunohistochemistry
experimental group400.120 ± 0.01015.3 ± 2.60.090 ± 0.01010.24 ± 1.55
control group400.022 ± 0.0033.1 ± 0.50.016 ± 0.0021.26 ± 0.33
P value< 0.05< 0.05< 0.05< 0.05
Tab.1  The expression of iNOS in villi
groupnumbershybridization in situimmunohistochemistry
experimental group400.003 ± 0.0010.28 ± 0.040.004 ± 0.0010.24 ± 0.05
control group400.003 ± 0.0030.3 ± 0.040.005 ± 0.0020.25 ± 0.03
P value> 0.05> 0.05> 0.05> 0.05
Tab.2  The expression of iNOS in deciduas
1 Srivastava M D, Thomas A, Srivastava B I, Check J H. Expression and modulation of progesterone induced blocking factor (PIBF) and innate immune factors in human leukemia cell lines by progesterone and mifepristone. Leuk Lymphoma , 2007, 48(8): 1610-1617
doi: 10.1080/10428190701471999
2 Kleiverda G. First trimester medical abortion: a good method that is seldom used in The Netherlands. Ned Tijdschr Geneeskd , 2006, 150(14): 766-769
3 Xia G Q, Sun Y Y. Expression of apoptosis and inducible nitric oxide synthase in chorion in early pregnancy. Reprod Contracept , 2001, 12(1): 56-60
4 Hausknecht R. Mifepristone and misoprostol for early medical abortion: 18 months experience in the United States. Contraception , 2003, 67(6): 463-465
doi: 10.1016/S0010-7824(03)00049-0
5 De Grandi P, Giudici G. Oral administration of an antiprogesterone (Mifepristone, RU 468) for preparing the cervix uteri for pregnancy interruption during the first trimester. J Gynecol Obstet Biol Reprod , 1989, 18(6): 801-808
6 Xia G Q, Xiong Y L, Sun Y Y. Effect of mifepristone on the telomerase activity in chorion and decidua during early gestation. Reprod Contracept , 2004, 15(4): 245-248
7 Lidegaard O, Larsen J F, Blaabjerg J, Larsen E. The first 100 early medical abortions. Ugeskr Laeger , 1999, 161(22): 3278-3281
8 Vervest H A, Haspels A A. Initial Dutch experiences with early pregnancy interruption using the antiprogesterone agent mifepriston. Ned Tijdschr Geneeskd , 1985, 129(35): 1680-1683
9 Papp C, Schatz F, Krikun G, Hausknecht V, Lockwood C J. Biological mechanisms underlying the clinical effects of mifepristone (RU 486) on the endometrium. Early Pregnancy , 2000, 4(4): 230-239
10 Paradisi R, Fabbri R, Battaglia C, Facchinetti F, Venturoli S. Nitric oxide levels in women with missed and threatened abortion: results of a pilot study. Fertil Steril , 2007, 88(3): 744-748
doi: 10.1016/j.fertnstert.2006.12.026
11 Marinoni E, Di Iorio R, Lucchini C, Di Netta T, Letizia C, Cosmi E V. Adrenomedullin and nitric oxide synthase at the maternal-decidual interface in early spontaneous abortion. J Reprod Med , 2004, 49(3): 153-161
12 H?m?l?inen M, Lilja R, Kankaanranta H, Moilanen E. Inhibition of iNOS expression and NO production by anti-inflammatory steroids. Reversal by histone deacetylase inhibitors. Pulm Pharmacol Ther , 2008, 21(2): 331-339
doi: 10.1016/j.pupt.2007.08.003
13 Urban G, Marinoni E, Di Iorio R, Lucchini C, Alo P, Di Tondo U. New placental factors: Between implantation and inflammatory reaction. Early Pregnancy , 2001, 5(1): 70-71
14 Eis A L, Brockman D E, Myatt L, Immunolocalization of the inducible nitric oxide synthase isoform in human fetal membranes. Am J Reprod Immunol , 1997, 38(4): 289-294
15 Zenclussen A C, Sollwedel A, Bertoja A Z, Gerlof K, Zenclussen M L, Woiciechowsky C, Volk H D. Heme oxygenase as a therapeutic target in immunological pregnancy complications. Int Immunopharmacol , 2005, 5(1): 41-51
doi: 10.1016/j.intimp.2004.09.011
16 Ogando D G, Paz D, Cella M, Franchi A M. The fundamental role of increased production of nitric oxide in lipopolysaccharide-induced embryonicresorption in mice. Reproduction , 2003, 125(1): 95-110
doi: 10.1530/rep.0.1250095
17 Matsumura M, Kakishita H, Suzuki M, Banba N, Hattori Y. Dexamethasone suppresses iNOS gene expression by inhibiting NF-kappaB in vascular smooth muscle cells. Life Sci , 2001, 69(9): 1067-1077
doi: 10.1016/S0024-3205(01)01196-1
18 Zhang D, Kishihara K, Wang B, Mizobe K, Kubo C, Nomoto K. Restraint stress-induced immunosuppression by inhibiting leukocyte migration and Th1 cytokine expression during the intraperitoneal infection of Listeria monocytogenes. J Neuroimmunol , 1998, 92(1,2): 139-151
19 Miller L, Alley E W, Murphy W J, Russell S W, Hunt J S. Progesterone inhibits inducible nitric oxide synthase gene expression and nitric oxide production in murine macrophages. J Leukoc Biol , 1996, 59(3): 442-450
20 Golde S, Coles A, Lindquist J A, Compston A. Decreased iNOS synthesis mediates dexamethasone-induced protection of neurons from inflammatory injury in vitro. Eur J Neurosci , 2003, 18(9): 2527-2537
doi: 10.1046/j.1460-9568.2003.02917.x
21 Xia G, Sun Y. Expression of the inducible nitric oxide synthase isoform in chorionic villi in the early spontaneous abortion. J Tongji Med Univ , 2000, 20(4): 338-339
22 Haddad E K, Duclos A J, Baines M G. Early embryo loss is associated with local production of nitric oxide by decidual mononuclear cells. J Exp Med , 1995, 182(4): 1143-1151
doi: 10.1084/jem.182.4.1143
23 Miech R P, Pathopharmacology of excessive hemorrhage in mifepristone abortions. Ann Pharmacother , 2007, 41(12): 2002-2007
doi: 10.1345/aph.1K351
24 V?is?nen-Tommiska M, Butzow R, Ylikorkala O, Mikkola T S. Mifepristone-induced nitric oxide release and expression of nitric oxide synthases in the human cervix during early pregnancy. Hum Reprod , 2006, 21(8): 2180-2184
doi: 10.1093/humrep/del141
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