|
|
Design of tandem genes cluster for isoflavone
engineering |
Xunli XIA PhD , Guangxiao YANG PhD , Guangyuan HE PhD , |
China-UK HUST-RRes Genetics
Engineering and Genomics Joint Laboratory, Genetic Engineering International
Cooperation Base of the Ministry of Science and Technology of China,
Key Laboratory of Molecular Biophysics of Chinese Ministry of Education,
College of Life Science and Technology, Huazhong University of Science
and Technology, Wuhan 430074, China; |
|
|
Abstract In this paper, a universal effective novel method of constructing tandem genes cluster for isoflavone engineering was reported. A tandem genes cluster CHS-CHI-IFS (rIFS) of secondary metabolites of plant isoflavones was constructed by using the chalcone synthase (CHS), chalcone isomerase (CHI), and isoflavone synthase (IFS) (GenBank accession numbers EU526827, EU526829, EU526830) in only one recombination with the pET22b vector. The resulting expression vector pET-rIFS was heterogeneously expressed and co-incubated with barrenwort extractions, and the genistein-like component was detected.
|
Keywords
isoflavones engineering
secondary metabolism
tandem genes cluster
|
Issue Date: 05 September 2009
|
|
|
Dixona R A, Ferreirab D. Genistein. Phytochemistry, 2002, 60(3): 205―211
doi: 10.1016/S0031-9422(02)00116-4
|
|
Mahmoud S S, Croteau R B. Metabolic engineering ofessential oil yield and composition in mint by altering expressionof deoxyxylulose phosphate reductoisomerase and menthofuran synthase. PNAS, 2001, 98(15): 8915―8920
doi: 10.1073/pnas.141237298
|
|
Chen Z, Young T E, Ling J, Chang S C, Gallie D R. Increasing vitamin C content of plantsthrough enhanced ascorbate recycling. PNAS, 2003, 10(6): 3525―3530
doi: 10.1073/pnas.0635176100
|
|
Paul D F, Susanne R, Cathie A S, Philippa B M, Joy W K, Norihiko M, Rachel G D, Wolfgang S, Peter M B. Evaluation of transgenic tomato plants expressing anadditional phytoene synthase in a fruit-specific manner. PNAS, 2002, 99(2): 1092―1097
doi: 10.1073/pnas.241374598
|
|
Zhang L, Ding R, Chai Y, Bonfill M, Moyano E, Xu T, Pi Y, Wang Z, Zhang H, Kai G, Liao Z, Sun X, Tang K. Engineering tropane biosynthetic pathwayin Hyoscyamus niger hairy root cultures. PNAS, 2004, 101(17): 6786―6791
doi: 10.1073/pnas.0401391101
|
|
Alekel D L, StGermain A, Pererson C T, Hanson K B, Stewart J W, Toda T. Isoflavone-rich soy protein isolate attenuates bone lossin the lumbar spine of perimenopausal women. Am J Clin Nutr, 2000, 72(3): 844―852
|
|
File S E, Jarrett N, Fluck E, Duffy R, Casey K, Wiseman H. Eatingsoya improves human memory. Psychopharmacology, 2001, 157(4): 430―436
doi: 10.1007/s002130100845
|
|
Fritz W A, Coward L, Wang J, Lamartiniere C A. Dietary genistein: perinatal mammary cancer prevention, bioavailabilityand toxicity testing in the rat. Carcinogenesis, 1998, 19(12): 2151―2158
doi: 10.1093/carcin/19.12.2151
|
|
Toshio A, Tomoyoshi A, Shin-ichi A. Flavonoids of Leguminous plants: Structure, biologicalactivity, and biosynthesis. J Plant Res, 2000, 113(4): 475―488
doi: 10.1007/PL00013958
|
|
Brenda W S. Flavonoid biosynthesis: A colorful model for genetics, biochemistry,cell biology, and biotechnology. PlantPhysiol, 2001, 126(2): 485―493
doi: 10.1104/pp.126.2.485
|
|
Liu C J, Jack W B, Christopher L S, Dixon R A. Bottlenecks for metabolic engineering of isoflavone glycoconjugatesin Arabidopsis. PNAS, 2002, 99(22): 14578―14583
doi: 10.1073/pnas.212522099
|
|
Yu O, Shi j, Hession A O , Maxwell C A , McGonigle B, Odell J T . Metabolic engineering to increase isoflavone biosynthesisin soybean seed. Phytochemistry, 2003, 63(7): 753―763
doi: 10.1016/S0031-9422(03)00345-5
|
|
Steele C L, Gijzen M, Qutob D, Dixon R A. Molecularcharacterization of the enzyme catalyzing the aryl migration reactionof isoflavonoid biosynthesis in soybean. Arch Biochem Biophys, 1999, 367(1): 147―150
doi: 10.1006/abbi.1999.1238
|
|
Van Adardt T G, van Rensburg H, Ferreira D. Synthesis of isoflavonoids. Enantiopure cis- and trans-6a-hydroxypterocarpansand a racemic trans-pterocarpan. Tetrahedron, 2001, 57(33): 7113―7126
doi: 10.1016/S0040-4020(01)00679-2
|
|
Vicario J L, Badia D, Dominguez E, Rodriquez M, Carrillo L. The first stereocontrolledsynthesis of isoflavanones. TetrahedronLett, 2000, 41(43): 8297―8300
doi: 10.1016/S0040-4039(00)01464-7
|
|
Roosien J, Belsham G J, Ryan M D, King A M Q, Vlak J M. Synthesis of foot-and-mouth disease viruscapsid proteins in insect cells using baculovirus expression vectors. J Gen Virol, 1990, 71(8): 1703―1711
doi: 10.1099/0022-1317-71-8-1703
|
|
Ryan M D, Drew J. Foot-and-mouth disease virus2A oligopeptide mediated cleavage of an artificial polyprotein. EMBO J, 1994, 13(4): 928―933
|
|
Suzuki N, Geletka L M, Nuss D L. Essential and dispensable virus-encoded replication elementsrevealed by efforts to develop hypoviruses as gene expression vectors. J Virol, 2000, 74(16): 7568―7577
doi: 10.1128/JVI.74.16.7568-7577.2000
|
|
de Felipe P, Hughes L E, Ryan M D, Brown J D. Co-translational,intraribosomal cleavage of polypeptides by the foot-and-mouth diseasevirus 2A peptide. J Biol Chem, 2003, 278(13): 11441―11448
doi: 10.1074/jbc.M211644200
|
|
Simoens C, Alliotte T, Mendel R, Muller A, Schiemann J, Van Lijsebettens M, Schell J, VanMontagu M, Inze D. A binary vector for transferring genomic libraries toplants. Nucleic Acids Res, 1986, 14(20): 8073―8090
doi: 10.1093/nar/14.20.8073
|
|
Hajdukiewicz P, Svab Z, Maliga P. The small, versatile pPZP family of Agrobacterium binaryvectors for plant transformation. PlantMol Biol, 1994, 25(6): 989―994
doi: 10.1007/BF00014672
|
|
Hamilton C M. A binary-BAC system for plant transformation with high-molecular-weightDNA. Gene, 1997, 200(2): 107―116
doi: 10.1016/S0378-1119(97)00388-0
|
|
Xiang C, Han P, Lutziger I, Wang K, Oliver D J. A mini binary vector series for planttransformation. Plant Mol Biol, 1999, 40(4): 711―717
doi: 10.1023/A:1006201910593
|
|
Hellens R P, Edwards E A, Leyland N R, Bean S, Mullineaux P M. pGreen: a versatile and flexiblebinary Ti vector for Agrobacteriummediated plant transformation. Plant Mol Biol, 2000b, 42(6): 819―832
doi: 10.1023/A:1006496308160
|
|
Earley K W, Haag J R, Pontes O, Opper K, Juehne T, Song K, Pikaard C S. Gateway-compatible vectorsfor plant functional genomics and proteomics. Plant J, 2006, 45(4): 616―629
doi: 10.1111/j.1365-313X.2005.02617.x
|
|
Halpin C, Barakate A, Askari B M, Abbott J C, Ryan M D. Enabling technologies formanipulating multiple genes on complex pathways. Plant Mol Biol, 2001, 47(2): 295―310
doi: 10.1023/A:1010604120234
|
|
Halpin C, Boerjan W. Stacking transgenes in foresttrees. Trends Plant Sci, 2003, 8(8): 363―365
doi: 10.1016/S1360-1385(03)00155-9
|
|
Capell T, Christou P. Progress in plant metabolicengineering. Curr Opin Biotechnol, 2004, 15(2): 148―154
doi: 10.1016/j.copbio.2004.01.009
|
|
Tyo K E, Alper H S, Stephanopoulos G N. Expanding the metabolic engineering toolbox:more options to engineer cells. TrendsBiotechnol, 2007, 25(3): 132―137
doi: 10.1016/j.tibtech.2007.01.003
|
|
Viewed |
|
|
|
Full text
|
|
|
|
|
Abstract
|
|
|
|
|
Cited |
|
|
|
|
|
Shared |
|
|
|
|
|
Discussed |
|
|
|
|