Please wait a minute...
Frontiers of Medicine

ISSN 2095-0217

ISSN 2095-0225(Online)

CN 11-5983/R

Postal Subscription Code 80-967

2018 Impact Factor: 1.847

Front. Med.    2010, Vol. 4 Issue (2) : 157-165     DOI: 10.1007/s11684-010-0034-5
Research articles |
Systems biomedical analysis of Schistosoma japonicum
Ze-Guang HAN MD,
Shanghai-MOST Key Laboratory for Disease and Health Genomics, Chinese National Human Genome Center at Shanghai, Shanghai 201203, China;Rui-Jin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China;
Download: PDF(169 KB)  
Export: BibTeX | EndNote | Reference Manager | ProCite | RefWorks
Abstract  Human schistosomiasis, caused mainly by three principal species including Schistosoma japonicum, S. mansoni, and S. hematobium, remains a major public health concern worldwide. S. japonicum is prevalent in southern China, being a major disease risk for 66 million people. The blood fluke has a complex life cycle for survival: as a free-living form in fresh water and as a parasite in the snail intermediate and vertebrate definitive hosts. Systems-based biomedical analyses, including genomic, transcriptomic, proteomic and metabonomic approaches, have been performed on the schistosome. These comprehensive investigations have not only characterized the genomic features but also chartered gene and protein expression profiles across genders and developmental stages. The integration of the huge information will lay a global and solid foundation for the molecular architecture of the biology, pathogenesis, and host-parasite interactions of the human blood fluke, which will facilitate the development of a new antischistosomal vaccine and drugs as well as diagnostic markers for the treatment and control of schistosomiasis.
Keywords Schistosoma japonicum      systems biomedical analysis      
Issue Date: 05 June 2010
URL:     OR
Engels D, Chitsulo L, Montresor A, Savioli L. The global epidemiological situation of schistosomiasisand new approaches to control and research. Acta Trop, 2002, 82(2): 139―146

doi: 10.1016/S0001-706X(02)00045-1
Gryseels B, Polman K, Clerinx J, Kestens L. Human schistosomiasis. Lancet, 2006, 368(9541): 1106―1118

doi: 10.1016/S0140-6736(06)69440-3
Haas W, Diekhoff D, Koch K, Schmalfuss G, Loy C. Schistosomamansoni cercariae: stimulation of acetabular gland secretion is adaptedto the chemical composition of mammalian skin. J Parasitol, 1997, 83(6): 1079―1085

doi: 10.2307/3284366
Haas W, Schmitt R. Characterizationof chemical stimuli for the penetration of Schistosoma mansoni cercariae.I. Effective substances, host specificity. Z Parasitenkd, 1982, 66(3): 293―307

doi: 10.1007/BF00925346
Abath F G C, Morais C N L, Montenegro C E L, Wynn T A, Montenegro S M L. Immunopathogenic mechanisms in schistosomiasis: What can be learntfrom human studies? Trends Parasitol, 2006, 22(2): 85―91

doi: 10.1016/
Amiri P, Locksley R M, Parslow T G, Sadick M, Rector E, Ritter D, McKerrow J H. Tumor necrosis factor alpha restores granulomas and inducesparasite egg-laying in schistosome-infected SCID mice. Nature, 1992, 356(6370): 604―607

doi: 10.1038/356604a0
Davies S J, Grogan J L, Blank R B, Lim K C, Locksley R M, McKerrow J H. Modulation of blood flukedevelopment in the liver by hepatic CD4+ lymphocytes. Science, 2001, 294(5545): 1358―1361

doi: 10.1126/science.1064462
de Mendonça R L, Escrivá H, Bouton D, Laudet V, Pierce R J. Hormones and nuclear receptors in schistosome development. Parasitol Today, 2000, 16(6): 233―240

doi: 10.1016/S0169-4758(00)01641-0
Ravindran B. Are inflammation and immunological hyperactivity neededfor filarial parasite development? TrendsParasitol, 2001, 17(2): 70―73

doi: 10.1016/S1471-4922(00)01835-3
Escobedo G, Roberts C W, Carrero J C, Morales-Montor J. Parasite regulation by host hormones:an old mechanism of host exploitation? Trends Parasitol, 2005, 21(12): 588―593

doi: 10.1016/
Schistosoma japonicumGenome Sequencing and Functional Analysis Consortium. The Schistosoma japonicum genome reveals features ofhost-parasite interplay. Nature, 2009, 460(7253): 345―351
Hu W, Yan Q, Shen D K, Liu F, Zhu Z D, Song H D, Xu X R, Wang Z J, Rong Y P, Zeng L C, Wu J, Zhang X, Wang J J, Xu X N, Wang S Y, Fu G, Zhang X L, Wang Z Q, Brindley P J, McManus D P, Xue C L, Feng Z, Chen Z, Han Z G. Evolutionary and biomedical implications of a Schistosoma japonicumcomplementary DNA resource. Nat Genet, 2003, 35(2): 139―147

doi: 10.1038/ng1236
Liu F, Lu J, Hu W, Wang S Y, Cui S J, Chi M, Yan Q, Wang X R, Song H D, Xu X N, Wang J J, Zhang X L, Zhang X, Wang Z Q, Xue C L, Brindley P J, McManus D P, Yang P Y, Feng Z, Chen Z, Han Z G. New perspectives on host-parasite interplay by comparative transcriptomicand proteomic analyses of Schistosoma japonicum. PLoS Pathog, 2006, 2(4): e29

doi: 10.1371/journal.ppat.0020029
Liu F, Hu W, Cui S J, Chi M, Fang C Y, Wang Z Q, Yang P Y, Han Z G. Insight into the host-parasite interplayby proteomic study of host proteins copurified with the human parasite,Schistosoma japonicum. Proteomics, 2007, 7(3): 450―462

doi: 10.1002/pmic.200600465
Liu F, Cui S J, Hu W, Feng Z, Wang Z Q, Han Z G. Excretory/secretory proteome of the adult developmental stage ofhuman blood fluke, Schistosoma japonicum. Mol Cell Proteomics, 2009, 8(6): 1236―1251

doi: 10.1074/mcp.M800538-MCP200
Huang J, Hao P, Chen H, Hu W, Yan Q, Liu F, Han Z G. Genome-wideidentification of Schistosoma japonicum microRNAs using a deep-sequencingapproach. PLoS One, 2009, 4(12): e8206

doi: 10.1371/journal.pone.0008206
Han Z G, Brindley P J, Wang S Y, Chen Z. Schistosoma genomics: new perspectives on schistosomebiology and host-parasite interaction. Annu Rev Genomics Hum Genet, 2009,10: 211―240

doi: 10.1146/annurev-genom-082908-150036
Brindley P J, Mitreva M, Ghedin E, Lustigman S. Helminth genomics: The implications for human health. PLoS Negl Trop Dis, 2009, 3(10): e538

doi: 10.1371/journal.pntd.0000538
Hu W, Brindley P J, McManus D P, Feng Z, Han Z G. Schistosometranscriptomes: new insights into the parasite and schistosomiasis. Trends Mol Med, 2004, 10(5): 217―225

doi: 10.1016/j.molmed.2004.03.002
Simpson A J, Sher A, McCutchan T F. The genome of Schistosomamansoni: isolation of DNA, its size, bases and repetitive sequences. Mol Biochem Parasitol, 1982, 6(2): 125―137

doi: 10.1016/0166-6851(82)90070-6
Johnston D A, Blaxter M L, Degrave W M, Foster J, Ivens A C, Melville S E. Genomics and the biologyof parasites. Bioessays, 1999, 21(2): 131―147

doi: 10.1002/(SICI)1521-1878(199902)21:2<131::AID-BIES7>3.0.CO;2-I
Franco G R, Valadão A F, Azevedo V, Rabelo E M. The Schistosoma gene discovery program: state of theart. Int J Parasitol, 2000, 30(4): 453―463

doi: 10.1016/S0020-7519(00)00020-5
Shrivastava J, Qian B Z, Mcvean G, Webster J P. An insight into the genetic variation of Schistosoma japonicum inmainland China using DNA microsatellite markers. Mol Ecol, 2005, 14(3): 839―849

doi: 10.1111/j.1365-294X.2005.02443.x
Hokke C H, Fitzpatrick J M, Hoffmann K F. Integrating transcriptome,proteome and glycome analyses of Schistosoma biology. Trends Parasitol, 2007, 23(4): 165―174

doi: 10.1016/
van Hellemond J J, van Balkom B W, Tielens A G. Schistosome biology and proteomics:progress and challenges. Exp Parasitol, 2007, 117(3): 267―274

doi: 10.1016/j.exppara.2007.05.004
Wilson R A, Ashton P D, Braschi S, Dillon G P, Berriman M, Ivens A. 'Oming in on schistosomes:prospects and limitations for post-genomics. Trends Parasitol, 2007, 23(1): 14―20

doi: 10.1016/
Cheng G F, Lin J J, Feng X G, Fu Z Q, Jin Y M, Yuan C X, Zhou Y C, Cai Y M. Proteomic analysis of differentiallyexpressed proteins between the male and female worm of Schistosomajaponicum after pairing. Proteomics, 2005, 5(2): 511―521

doi: 10.1002/pmic.200400953
McLaren D J, Hockley D J. Blood flukes have a double outer membrane. Nature, 1977, 269(5624): 147―149

doi: 10.1038/269147a0
Jenkins S J, Hewitson J P, Jenkins G R, Mountford A P. Modulation of the host's immune response by schistosomelarvae. Parasite Immunol, 2005, 27(10―11): 385―393
Lightowlers M W, Rickard M D. Excretory-secretory products of helminth parasites: effects on hostimmune responses. Parasitology, 1988, 96(Suppl): S123―166
Nicholson J K, Lindon J C. Systems biology – metabonomics. Nature, 2008, 455 (7216): 1054―1056

doi: 10.1038/4551054a
Wang Y, Holmes E, Nicholson J K, Cloarec O, Chollet J, Tanner M, Singer B H, Utzinger J. Metabonomicinvestigations in mice infected with Schistosoma mansoni: an approachfor biomarker identification. Proc NatlAcad Sci USA, 2004, 101(34): 12676―12681

doi: 10.1073/pnas.0404878101
Wang Y, Utzinger J, Xiao S H, Xue J, Nicholson J K, Tanner M, Singer B H, Holmes E. Systemlevel metabolic effects of a Schistosoma japonicum infection in theSyrian hamster. Mol Biochem Parasitol, 2006, 146(1): 1―9

doi: 10.1016/j.molbiopara.2005.10.010
Wu J F, Holmes E, Xue J, Xiao S H, Singer B H, Tang H R, Utzinger J, Wang Y L. Metabolicalterations in the hamster co-infected with Schistosoma japonicumand Necator americanus. Int J Parasitol, 2010, 40(6): 695―703

doi: 10.1016/j.ijpara.2009.11.003
Skelly P J, Kim J W, Cunningham J, Shoemaker C B. Cloning, characterization, and functional expressionof cDNAs encoding glucose transporter proteins from the human parasiteSchistosoma mansoni. J Biol Chem, 1994, 269(6): 4247―4253
Skelly P J, Shoemaker C B. Rapid appearance and asymmetric distribution of glucose transporterSGTP4 at the apical surface of intramammalian-stage Schistosoma mansoni. Proc Natl Acad Sci U S A, 1996, 93(8): 3642―3646

doi: 10.1073/pnas.93.8.3642
Cohen L M, Neimark H, Eveland L K. Schistosoma mansoni: responseof cercariae to a thermal gradient. J Parasitol, 1980, 66(2): 362―364

doi: 10.2307/3280843
Maizels R M, Bundy D A, Selkirk M E, Smith D F, Anderson R M. Immunological modulationand evasion by helminth parasites in human populations. Nature, 1993, 365(6449): 797―805

doi: 10.1038/365797a0
McKerrow J H. Cytokine induction and exploitation in schistosome infections. Parasitology, 1997, 115(Suppl): 107―112

doi: 10.1017/S0031182097001765
Dvorák J, Mashiyama S T, Braschi S, Sajid M, Knudsen G M, Hansell E, Lim K C, Hsieh I, Bahgat M, Mackenzie B, Medzihradszky K F, Babbitt P C, Caffrey C R, McKerrow J H. Differential use of proteasefamilies for invasion by schistosome cercariae. Biochimie, 2008, 90(2): 345―358

doi: 10.1016/j.biochi.2007.08.013
Koehler J W, Morales M E, Shelby B D, Brindley P J. Aspartic protease activities of schistosomes cleave mammalianhemoglobins in a host-specific manner. Mem Inst Oswaldo Cruz, 2007, 102(1): 83―85

doi: 10.1590/S0074-02762007000100014
Zhou X N, Wang L Y, Chen M G, Wu X H, Jiang Q W, Chen X Y, Zheng J, Utzinger J. The public health significance and controlof schistosomiasis in China- then and now. Acta Trop, 2005, 96(2―3): 97―105

doi: 10.1016/j.actatropica.2005.07.005
Utzinger J, Zhou X N, Chen M G, Bergquist R. Conqueringschistosomiasis in China: the long march. Acta Trop, 2005, 96(2―3): 69―96
Li Y S, Raso G, Zhao Z Y, He Y K, Ellis M K, McManus D P. Large water management projects and schistosomiasis control,Dongting Lake region, China. Emerg InfectDis, 2007, 13(7): 973―979
Bergquist R, Utzinger J, McManus D P. Trick or treat: the roleof vaccines in integrated schistosomiasis control. PLoS Negl Trop Dis, 2008, 2(6): e244

doi: 10.1371/journal.pntd.0000244
Ismail M, Botros S, Metwally A, William S, Farghally A, Tao L F, Day T A, Bennett J L. Resistance to praziquantel: direct evidence from Schistosoma mansoniisolated from Egyptian villagers. Am JTrop Med Hyg, 1999, 60(6): 932―935
Stelma F F, Talla I, Sow S, Kongs A, Niang M, Polman K, Deelder A M, Gryseels B. Efficacy and side effectsof praziquantel in an epidemic focus of Schistosoma mansoni. Am J Trop Med Hyg, 1995, 53(2): 167―170
Doenhoff M J, Cioli D, Utzinger J. Praziquantel: mechanisms of action, resistanceand new derivatives for schistosomiasis. Curr Opin Infect Dis, 2008, 21(6): 659―667

doi: 10.1097/QCO.0b013e328318978f
Gourlay L J, Angelucci F, Baiocco P, Boumis G, Brunori M, Bellelli A, Miele A E. The three-dimensional structure of two redox states ofcyclophilin A from Schistosoma mansoni. Evidence for redox regulationof peptidyl-prolyl cis-trans isomerase activity. J Biol Chem, 2007, 282(34): 24851―24857

doi: 10.1074/jbc.M702714200
Sayed A A, Simeonov A, Thomas C J, Inglese J, Austin C P, Williams D L. Identification of oxadiazolesas new drug leads for the control of schistosomiasis. Nat Med, 2008, 14(4): 407―412

doi: 10.1038/nm1737
Kuntz A N, Davioud-Charvet E, Sayed A A, Califf L L, Dessolin J, Arnér E S, Williams D L. Thioredoxin glutathione reductase from Schistosoma mansoni:an essential parasite enzyme and a key drug target. PLoS Med, 2007, 4(6): e206

doi: 10.1371/journal.pmed.0040206
Wu W, Niles E G, Hirai H, LoVerde P T. Evolution of a novel subfamily of nuclear receptors with membersthat each contain two DNA binding domains. BMC Evol Biol, 2007, 7: 27

doi: 10.1186/1471-2148-7-27
Freitas T C, Jung E, Pearce E J. TGF-beta signaling controls embryo developmentin the parasitic flatworm Schistosoma mansoni. PLoS Pathog, 2007, 3(4): e52

doi: 10.1371/journal.ppat.0030052
Osman A, Niles E G, Verjovski-Almeida S, LoVerde P T. Schistosoma mansoni TGF-beta receptor II: role in hostligand-induced regulation of a schistosome target gene. PLoS Pathog, 2006, 2(6): e54

doi: 10.1371/journal.ppat.0020054
Morales M E, Rinaldi G, Gobert G N, Kines K J, Tort J F, Brindley P J. RNA interference of Schistosomamansoni cathepsin D, the apical enzyme of the hemoglobin proteolysiscascade. Mol Biochem Parasitol, 2008, 157(2): 160―168

doi: 10.1016/j.molbiopara.2007.10.009
Correnti J M, Brindley P J, Pearce E J. Long-term suppression ofcathepsin B levels by RNA interference retards schistosome growth. Mol Biochem Parasitol, 2005, 143(2): 209―215

doi: 10.1016/j.molbiopara.2005.06.007
McManus D P, Loukas A. Thecurrent status of vaccines for schistosomiasis. Clin Microbiol Rev, 2008, 21(1): 225―242

doi: 10.1128/CMR.00046-07
Loukas A, Tran M, Pearson M S. Schistosome membrane proteins as vaccines. Int J Parasitol, 2007, 37(3―4): 257―263

doi: 10.1016/j.ijpara.2006.12.001
Tran M H, Pearson M S, Bethony J M, Smyth D J, Jones M K, Duke M, Don T A, McManus D P, Correa-Oliveira R, Loukas A. Tetraspaninson the surface of Schistosoma mansoni are protective antigens againstschistosomiasis. Nat Med, 2006, 12(7): 835―840

doi: 10.1038/nm1430
Hotez P J, Molyneux D H, Fenwick A, Kumaresan J, Sachs S E, Sachs J D, Savioli L. Controlof neglected tropical diseases. N EnglJ Med, 2007, 357(10): 1018―1027

doi: 10.1056/NEJMra064142
Wang L D, Chen H G, Guo J G, Zeng X J, Hong X L, Xiong J J, Wu X H, Wang X H, Wang L Y, Xia G, Hao Y, Chin D P, Zhou X N. A strategy to control transmission ofSchistosoma japonicum in China. N EnglJ Med, 2009, 360(2): 121―128

doi: 10.1056/NEJMoa0800135
Bergquist R, Johansen M V, Utzinger J. Diagnostic dilemmas in helminthology:what tools to use and when? Trends Parasitol, 2009, 25(4): 151―156

doi: 10.1016/
No related articles found!
Full text