Effects of regulators on the respiratory enzyme of pear branches during dormant period
Effects of regulators on the respiratory enzyme of pear branches during dormant period
Lei BI1, Yuxing ZHANG1(), Bharat Kumar POUDYAL2, Xiaolei WU1, Jie LIU1
1. College of Horticulture, Agricultural University of Hebei, Baoding 071001, China; 2. Department of Agriculture, Fruit Development Directorate, Kirtipur, Kathmandu, Nepal
The effects of regulators on the respiratory enzymes of Qiyuesu’s pear branches during dormant period were studied. The results showed that enzyme activities of SDH, G-6-PDH and 6-PGDH were increased by 0.02 mmol/L SA and 120 mg/L GA3. The enzyme activities of phosphohexoisomerase were reduced by 0.02 mmol/L SA and 120 mg/L GA3. Compared with the control, the PGI activity was reduced by 20.5% and 13.6% using 0.02 mmol/L SA and 120 mg/L GA3. Similarly, the SDH activity was increased by 6.1% and 29.2%, respectively. Likewise, the activity of G-6-PDH and 6-PGDH was increased by 93.9% and 24.8%. Changes of respiratory enzymes were consistent with respiratory pathway of regulators, which indicated that SA and GA3 were helpful to break the dormancy by enhancing activities of SDH, G-6-PDH and 6-PGDH.
. Effects of regulators on the respiratory enzyme of pear branches during dormant period[J]. Frontiers of Agriculture in China, 2011, 5(4): 538-542.
Lei BI, Yuxing ZHANG, Bharat Kumar POUDYAL, Xiaolei WU, Jie LIU. Effects of regulators on the respiratory enzyme of pear branches during dormant period. Front Agric Chin, 2011, 5(4): 538-542.
Bewley J D, Black M (1994). Seeds: Physiology of Development and Germination. 2nd ed. New York: Plenum, 462
2
Bogatek R, Rychter A (1984). Respiratory activity of apple seeds during dormancy removal and germination. Physiol Veg , 22: 181–191
3
Brown A P, Wary J L (1968). Correlated change of some enzyme activities and cofactor and substrate contents of pea cotyledon tissue during germination. Biochem J , 108: 437–444
4
Erez A (1971). Improved methods for breaking rest in peach and other deciduous fruit species. Jour Am Soc Hort Sci , 96: 519–522
5
Faust M, Erez A, Rowland L J (1997). Bud dormancy in perennial fruit trees; physiological basis for dormancy induction maintenance and release. HortScience , 32: 623–629
6
Fiehn O, Kopka J, Dormann P, Altmann T, Trethewey R N, Willmitzer L (2000). Metabolite profiling for plant functional genomics. Nat Biotechnol , 18(11): 1157–1161 doi: 10.1038/81137
7
Finch-Savage W E, Leubner-Metzger G (2006). Seed dormancy and the control of germination. New Phytol , 171(3): 501–523 doi: 10.1111/j.1469-8137.2006.01787.x
8
Fuchigami L H, Wisniewski M (1997). Quantifying bud dormancy: physiological approaches. HortScience , 32: 618–632
9
Hiatt A J (1961). Preparation and some properties of soluble succinic dehydroge- nase from higher plants. Plant Physiol , 36(5): 552–557 doi: 10.1104/pp.36.5.552
10
Hilhorst H W M (1995). A critical update on seed dormancy. I. Primary dormancy. Seed Sci Res , 5(02): 61–73 doi: 10.1017/S0960258500002634
Karssen C M, Zagorski Z, Kepczynski J, Groot S P C (1989). Key role for gibbere11ins in the control of seed germination. Ann Bot (Lond) , 63: 71–80
13
Kong F X, Hu W, Chao S Y, Sang W L, Wang L S (1999). Physiological responses of lichen Xanthoparmelia Mexicana to oxidative stress of SO2. Environ Exp Bot , 42(3): 201–209 doi: 10.1016/S0098-8472(99)00034-9
14
Kuroda H, Sagisaka S, Asada M, Chiba K (1991). Seasonal variation in the activities of NADH-cytochrome c reductase and cytochrome c oxidase in plastids, mitochon-dria and microsomes in apple trees. J Japan Soc Hort Sci , 60(2): 457–466 doi: 10.2503/jjshs.60.457
15
Lang G A (1994). Dormancy-The missing links: Molecular studies and integration of regulatory plant and environmental interactions. HortScience , 29: 1255–1263
16
Leslie C A, Romani R J (1986). Salicylic acid: a new inhibitor of ethylene biosynthesis. Plant Cell Rep , 5(5): 144–146 doi: 10.1007/BF00269255
17
Li B, Foley M E (1995). Cloning and characterization of differentially expressed genes in imbibed dormant and after ripened. Avena fatua embryos. Plant Mol Biol , 29(4): 823–831 doi: 10.1007/BF00041171
18
Li Q, Wu Y Y (2004). Biochemistry. Beijing: China Agriculture Press, 143–165 (in Chinese)
19
Li X (2004). Control of respiratory metabolism on sweat cherry and studies on technical of dormancy release. The Master's Dissertation . Tai’an: Shandong Agricultural University (in Chinese)
20
Li X L, Yuan Z Y, Gao D S (2001). Factors that influence bud dormancy in deciduous fruit trees. J Shandong Univ Nat Sci , 32(3): 386–392 (in Chinese)
21
Neales T F, Incoll I D (1968). The control of leaf photosynthesis rate by the level of assimilate concentration in the leaf: a review of the hypothesis. Bot Rev , 34(2): 107–125 doi: 10.1007/BF02872604
22
Nir G, Lavee S (1993). Metabolic changes during cyanimide induced dormancy release in grapevines. Acta Hortic , 329: 271–274
23
Wang S Y, Jiao J, Faust M (1991). Changes in metabolic enzyme activities during thidiazuron-induced lateral bud break of apple. HortScience , 26: 171–173