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Frontiers of Agriculture in China

ISSN 1673-7334

ISSN 1673-744X(Online)

CN 11-5729/S

Frontiers of Agriculture in China  2010, Vol. 4 Issue (4): 507-512   https://doi.org/10.1007/s11703-010-1048-0
  RESEARCH ARTICLE 本期目录
Effect of various dietary vitamin A levels on growth performance and immune response of tilapia (Oreochromis niloticus)
Effect of various dietary vitamin A levels on growth performance and immune response of tilapia (Oreochromis niloticus)
Ran GUO1,2(), Chhorn LIM2, Hui XIA1,2, Mediha Yildirim AKSOR2, Menghe LI3
1. Ocean College, Agricultural University of Hebei, Qinhuangdao 066000, China; 2. Aquatic Animal Health Research Laboratories, Agricultural Research Service, USDA, 990 Wire Road, Auburn, AL, 36830, USA; 3. Thad Cochran National Warmwater Aquaculture Center, Mississippi State University, Stoneville, MS 38776 USA
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Abstract

Six purified vitamin-free casein-based diets were formulated to contain six levels vitamin A at 0, 500, 1000, 2000, 4000, and 8000 IU·kg-1, respectively. Tilapias (initial mean weight: 7.73±0.03 g) were fed the diets in quadruplicate aquaria to apparent satiation twice daily for 10 weeks. No differences in mortality, weight gain, or feed efficiency ratio (FER) were observed among the groups. Liver vitamin A levels reflected dietary vitamin A levels. Immune parameters, such as hemoglobin levels, total cell count, red blood cell count, total serum protein, and serum lysozyme activity, did not vary with the dietary vitamin A levels. White blood cell counts of fish in 2000 IU·kg-1 diet groups were significantly higher than that in other diets groups. Serum complement activities of fish in 2000 and 4000 IU·kg-1 vitamin A diet groups were also higher than those in other diet groups. After the 14-d challenge test, the mortality and antibody titer were similar among the treatments. The results indicated that dietary vitamin A inclusions did not affect the immune response of Oreochromis niloticus.

Key wordsvitamin A    health    immune response    tilapia (Oreochromis niloticus)
收稿日期: 2010-06-25      出版日期: 2010-12-05
Corresponding Author(s): GUO Ran,Email:toguoran@163.com   
 引用本文:   
. Effect of various dietary vitamin A levels on growth performance and immune response of tilapia (Oreochromis niloticus)[J]. Frontiers of Agriculture in China, 2010, 4(4): 507-512.
Ran GUO, Chhorn LIM, Hui XIA, Mediha Yildirim AKSOR, Menghe LI. Effect of various dietary vitamin A levels on growth performance and immune response of tilapia (Oreochromis niloticus). Front Agric Chin, 2010, 4(4): 507-512.
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https://academic.hep.com.cn/fag/CN/10.1007/s11703-010-1048-0
https://academic.hep.com.cn/fag/CN/Y2010/V4/I4/507
ingredientspercent in diet
casein vitamin-free32
gelatin8
cornstarch33
cod liver oil3
soybean oil3
mineral premix14
vitamin premix2 (vitamin A free)1
CMC (carboxymethyl cellulose sodium)3
cellulose313
analyzed nutrients
crude protein/%35.6
crude fat/%5.6
estimated digestible energy/kcal·g-13.2
Tab.1  
vitamin A added/ (IU·kg-1)weight gain/%dry matter feed intake/(g·fish-1)FER3survival/%
0594.17±96.1548.27±5.340.96±0.0698.57±1.65
500590.70±83.0847.73±2.910.97±0.0897.88±1.42
1000593.15±54.7546.80±2.291.00±0.05100.00±2.33
2000575.45±21.8645.60±1.160.98±0.0399.33±3.52
4000622.89±62.7647.56±3.491.01±0.0394.29±2.33
8000640.49±59.9047.84±2.961.03±0.0399.29±1.43
Tab.2  
vitamin A added/(IU·kg-1)serum protein/(mg·mL-1)lysozyme/(mg·mL-1)alternative complement AH50/(unit·mL-1)antibody titer
035.26±1.7157.18±17.6539.70±25.59a1.72±0.52
50035.29±2.4859.12±13.0155.56±30.67ab1.47±0.33
100035.42±1.0767.32±13.2175.37±47.02b1.50±0.32
200036.01±3.5153.99±20.5574.92±67.80b1.47±0.65
400034.85±0.8271.28±6.9648.38±28.41ab1.57±0.31
800032.93±3.2457.25±18.1354.13±32.36ab2.09±0.62
Tab.3  
vitamin A added/ (IU·kg-1)hematocrit/%total cell count/(106·μL-1)red blood cell/(106·μL-1)white blood cell/(105·μL-1)hemoglobin/(g·dL-1)
030.6±1.672.26±0.131.99±0.092.73±0.52ab8.21±0.33
50031.98±3.132.26±0.062.01±0.092.45±0.44ab8.45±0.40
100030.58±2.242.41±0.382.12±0.402.93±0.30b8.30±0.79
200031.03±3.092.13±0.341.88±0.332.49±0.23ab8.55±0.43
400030.43±3.102.33±0.172.08±0.152.53±0.59ab8.36±0.28
800030.78±2.702.11±0.221.89±0.192.22±0.35a8.60±0.29
Tab.4  
vitamin A added/(IU·kg-1)cumulative mortality/%
087.00±6.83
50083.88±4.48
100088.00±8.64
200084.00±5.66
400081.00±17.40
800080.00±8.64
Tab.5  
vitamin A added/(IU·g-1)vitamin A content in diet/(IU·g-1)vitamin A content in liver/(IU·g-1)
024,31065,312a
50024,71582,021a
100025,916128,603b
200039,626132,602b
400040,979140,064b
800045,985187,965c
Tab.6  
Fig.1  
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