Transcriptome resources and genome-wide marker development for Japanese larch (Larix kaempferi)

doi:10.15302/J-FASE-2014010

Frontiers of Agricultural Science and Engineering

2014, Vol. 1

Issue (1): 77-84 https://doi.org/10.15302/J-FASE-2014010

本期目录

Transcriptome resources and genome-wide marker development for Japanese larch (Larix kaempferi)

Wanfeng LI¹,Suying HAN²,Liwang QI¹,Shougong ZHANG^1,^*(

)

1. State Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China
2. State Key Laboratory of Tree Genetics and Breeding, Research Institute of Forest Ecology, Environment and Protection, Chinese Academy of Forestry, Beijing 100091, China

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Abstract：

While the differential responses of trees to changes in climatic and environmental conditions have been demonstrated as they age, the underlying mechanisms and age control of tree growth and development are complex and poorly understood particularly at a molecular level. In this paper, we present a transcriptome analysis of Larix kaempferi, a deciduous conifer that is widely-grown in the northern hemisphere and of significant ecological and economic value. Using high-throughput RNA sequencing, we obtained about 26 million reads from the stems of 1-, 2-, 5-, 10-, 25- and 50-year-old L. kaempferi trees. Combining these with the published Roche 454 sequencing reads and the expressed sequence tags (both mainly from Larix embryogenic cell cultures), we assembled 26670549 reads into 146786 transcripts, of which we annotated 79182 to support investigations of the molecular basis of tree aging and adaption, somatic embryogenesis and wood formation. Using these sequences we also identified many single-nucleotide polymorphisms, simple sequence repeats, and insertion and deletion markers to assist breeding and genetic diversity studies of Larix.

Key words： Larix transcriptome age wood formation somatic embryogenesis molecular marker

收稿日期: 2014-04-10 出版日期: 2014-05-22

Corresponding Author(s): Shougong ZHANG

引用本文:

. [J]. Frontiers of Agricultural Science and Engineering, 2014, 1(1): 77-84.
Wanfeng LI,Suying HAN,Liwang QI,Shougong ZHANG. Transcriptome resources and genome-wide marker development for Japanese larch (Larix kaempferi). Front. Agr. Sci. Eng. , 2014, 1(1): 77-84.

链接本文:

https://academic.hep.com.cn/fase/CN/10.15302/J-FASE-2014010
https://academic.hep.com.cn/fase/CN/Y2014/V1/I1/77

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