Dynamics of foot-and-mouth disease virus replication in cells at different phases of the cell-division cycle

doi:10.15302/J-FASE-2014031

Frontiers of Agricultural Science and Engineering

2014, Vol. 1

Issue (3): 250-257 https://doi.org/10.15302/J-FASE-2014031

本期目录

Dynamics of foot-and-mouth disease virus replication in cells at different phases of the cell-division cycle

Claudia DOEL¹,Zhidong ZHANG^1,^2,^*(

),Lise MAZELET¹,Ryan WATERS¹,John BASHIRUDDIN¹

1. Pirbright Institute, Ash Road, Pirbright, Woking, Surrey, GU24 0NF, United Kingdom
2. State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Lanzhou730046, China

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Abstract：

Foot-and-mouth-disease virus (FMDV) replicates in epithelial cells. The restriction of FMDV RNA to the basal cell layer of epithelia suggests a possible link between FMDV replication in vivo and the cell status. This paper describes in vitro studies in which FMDV infection was investigated in cells that were held at various cell division phases using cell cycle inhibitors. The results suggest that when cells were arrested at the G₁ or G₁/S phase, high levels of viral RNA were detected by quantitative real-time reverse transcription PCR and viral protein synthesis was observed by specific labeling techniques. In contrast, when cells were arrested at the G₂/M phase, reduced or no viral RNA synthesis was detected.

Key words： foot-and-mouth disease virus cell cycle replication

收稿日期: 2014-11-13 出版日期: 2015-01-27

Corresponding Author(s): Zhidong ZHANG

引用本文:

. [J]. Frontiers of Agricultural Science and Engineering, 2014, 1(3): 250-257.
Claudia DOEL,Zhidong ZHANG,Lise MAZELET,Ryan WATERS,John BASHIRUDDIN. Dynamics of foot-and-mouth disease virus replication in cells at different phases of the cell-division cycle. Front. Agr. Sci. Eng. , 2014, 1(3): 250-257.

链接本文:

https://academic.hep.com.cn/fase/CN/10.15302/J-FASE-2014031
https://academic.hep.com.cn/fase/CN/Y2014/V1/I3/250

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