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Frontiers of Agricultural Science and Engineering

ISSN 2095-7505

ISSN 2095-977X(Online)

CN 10-1204/S

邮发代号 80-906

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Frontiers of Agricultural Science and Engineering  2015, Vol. 2 Issue (1): 90-99   https://doi.org/10.15302/J-FASE-2015045
  本期目录
Metabonomic study of the biochemical profiles of heterozygous myostatin knockout swine
Jianxiang XU1,Dengke PAN2,Jie ZHAO1,Jianwu WANG1,Xiaohong HE2,Yuehui MA2,Ning LI1,*()
1. State Key Laboratory for Agrobiotechnology, China Agricultural University, Beijing 100193, China
2. Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China
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Abstract：

Myostatin is a transforming growth factor-β family member that normally acts to limit skeletal muscle growth. Myostatin gene (MSTN) knockout (KO) mice show possible effects for the prevention or treatment of metabolic disorders such as obesity and type 2 diabetes. We applied chromatography and mass spectrometry based metabonomics to assess system-wide metabolic response of heterozygous MSTN KO (MSTN+/-) swine. Most of the metabolic data for MSTN+/- swine were similar to the data for wild type (WT) control swine. There were, however, metabolic changes related to fatty acid metabolism, glucose utilization, lipid metabolism, as well as BCAA catabolism caused by monoallelic MSTN depletion.The statistical analyses suggested that: (1) most metabolic changes were not significant in MSTN+/- swine compared to WT swine; (2) only a few metabolic properties were significantly different between KO and WT swine, especially for lipid metabolism. Significantly, these minor changes were most evident in female KO swine and suggested differences in gender sensitivity to myostatin.
Key words： myostatin    transforming growth factor-β family    skeletal muscle    metabolic disorders    chromatography    mass spectrometry    metabonomics
收稿日期: 2015-02-04      出版日期: 2015-05-22
Corresponding Author(s): Ning LI   
 引用本文:   
. [J]. Frontiers of Agricultural Science and Engineering, 2015, 2(1): 90-99.
Jianxiang XU,Dengke PAN,Jie ZHAO,Jianwu WANG,Xiaohong HE,Yuehui MA,Ning LI. Metabonomic study of the biochemical profiles of heterozygous myostatin knockout swine. Front. Agr. Sci. Eng. , 2015, 2(1): 90-99.
 链接本文:  
https://academic.hep.com.cn/fase/CN/10.15302/J-FASE-2015045
https://academic.hep.com.cn/fase/CN/Y2015/V2/I1/90
Group Sample Bodyweight (mean±SD)/kg Generation Description
WT Male (n = 11) 114.36±4.02 F1 Wild type adult swine (MSTN +/+)
Female (n = 3) 113.92±5.40
KO Male (n = 7) 113.74±5.35 F1 Heterozygous MSTN KO adult swine (MSTN+/-)
Female (n = 5) 114.43±5.24
Tab.1  
Fig.1  
Item KO (MSTN+/-) WT (MSTN +/+) P
Male Female Male Female Male Female
WBC/( × 109·L-1) 20.8 (4.8) 23.5 (1.0) 26.1 (6.8) 25.4 (7.4) 0.0923 0.6984
RBC/( × 1012·L-1) 6.3 (0.8) 6.9 (0.5) 6.9 (0.5) 6.0 (0.7) 0.1446 0.1387
HGB/(g·L-1) 106 (12.8) 120.2 (7.8) 117.8 (3.8) 107 0.0 (15.9) 0.0742 0.2838
HCT/% 34.7 (4.4) 39.1 (2.9) 38.1 (2.0) 34.3 (4.1) 0.1221 0.1666
MCV/fL 54.9 (1.2) 56.4 (2.5) 55.2 (2.4) 56.7 (1.0) 0.7692 0.8638
MCH/pg 16.8 (1.0) 17.4 (1.1) 17.1 (1.2) 17.6 (0.7) 0.5846 0.7050
MCHC/(g·L-1) 307 (22.9) 308 (10.3) 310.3 (15.2) 312.0 (15.0) 0.7272 0.7094
PLT/( × 109·L-1) 228 (119.7) 321 (103.7) 329.1 (103.2) 351.7 (92.4) 0.1933 0.6870
TBIL/(mmol·L-1) 3.57 (2.44) 4.80 (3.11) 3.10 (1.91) 1.00 (0) 0.6773 0.0525
DBIL/(mmol·L-1) 12.14 (1.46) 12.80 (2.17) 12.60 (1.17) 11.33 (0.58) 0.5067 0.2135
ALT/(U·L-1) 56.43 (11.31)a 69.60 (9.91) 71.70 (9.15)a 58.00 (7.21) 0.0128 0.1091
AST/(U·L-1) 113.00 (83.66) 83.20 (7.76) 121.40 (63.12) 71.33 (34.02) 0.8265 0.6091
TP/(g·L-1) 63.03 (8.08) 69.82 (2.19) 73.24 (5.75) 71.23 (9.38) 0.0164 0.8200
ALB/GLO 1.51 (0.32) 1.60 (0.19) 1.43 (0.17) 1.27 (0.24) 0.5586 0.1297
GGT/(U·L-1) 37.14 (9.42) 110.80 (40.54) 40.90 (16.35) 40.33 (19.09) 0.5587 0.0164
ALP/(U·L-1) 19.43 (17.92) 10.00 (7.91) 11.40 (7.03) 5.33 (3.21) 0.2956 0.2895
BUN/(mmol·L-1) 4.06 (1.45) 4.61 (0.45) 4.55 (0.90) 4.85 (1.25) 0.4515 0.7780
CRE/(mmol·L-1) 156 (17.72) 152 (10.92) 145 (19.46) 152 (28.36) 0.2551 0.9972
GLU/(mmol·L-1) 4.27 (1.81) 3.43 (0.67) 4.62 (1.32) 3.99 (0.43) 0.6700 0.2018
TG/(mmol·L-1) 0.56 (0.24) 0.52 (0.13) 0.40 (0.09) 0.36 (0.12) 0.1367 0.1372
CHO/(mmol·L-1) 1.94 (0.33) 2.33 (0.15) 2.08 (0.19) 2.10 (0.15) 0.3294 0.0899
CK/(U·L-1) 2215 (750.5) 2014.00 (1121.3) 1726 (769.7) 758 (321.9) 0.2122 0.0659
LDH/(U·L-1) 731 (221.3)a 894 (78.3)b 1170 (531.3)a 475 (42.0)b 0.0362 0.0001
AMY/(U·L-1) 2442 (962.5) 3160 (1126.1) 2179 (1028.3) 1857 (976.0) 0.5989 0.1463
Tab.2  
Biochemicals KO/WT KO-M/WT-M KO-F/WT-F
Total (P≤0.05) 43 10 35
Fold of change≥1.00 29 2 25
Fold of change<1.00 14 8 10
Tab.3  
Fig.2  
Fig.3  
Fig.4  
Fig.5  
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