Effect of calcium on porcine ICSI embryos expressing EGFP is related to activation of ooplasmic DNase I

doi:10.15302/J-FASE-2015046

Frontiers of Agricultural Science and Engineering

2015, Vol. 2

Issue (1): 84-89 https://doi.org/10.15302/J-FASE-2015046

本期目录

Effect of calcium on porcine ICSI embryos expressing EGFP is related to activation of ooplasmic DNase I

Shuaishuai WU,Heng CHEN,Yingzheng WANG,Hui GAO,Shenming ZENG(

)

Laboratory of Animal Embryonic Biotechnology, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China

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Abstract：

Several reliable methods to produce transgenic animals use the male genome. After penetration into oocytes, sperm DNA undergoes dramatic conformational changes that might represent an opportunity for exogenous DNA to integrate into the zygote genome. A nuclease, DNase I, with Ca²⁺/Mg²⁺ dependent activity and Zn²⁺ inhibition, is one of the enzymes responsible for sperm DNA remodeling. To date, the effect of different calcium concentrations in manipulation media on porcine intracytoplasmic sperm injection has not been fully investigated. The present study was conducted to examine the effect of calcium in the surrounding media, and we found that the number of embryos expressing green fluorescent protein (EGFP) was increased in media containing Ca²⁺. However, the number did not change over Ca²⁺ concentrations from 2 to 8 mmol·L^–1 (P>0.05). Moreover, free Ca²⁺ concentrations in the media were found to affect the efficiency which is ICSI embryos expressing EGFP protein, which was related to the activation of ooplasmic DNase I. These findings reveal a mechanism and pathway involving EGFP expression in ICSI embryos.

Key words： ICSI calcium DNase I GFP porcine

收稿日期: 2015-01-08 出版日期: 2015-05-22

Corresponding Author(s): Shenming ZENG

引用本文:

. [J]. Frontiers of Agricultural Science and Engineering, 2015, 2(1): 84-89.
Shuaishuai WU,Heng CHEN,Yingzheng WANG,Hui GAO,Shenming ZENG. Effect of calcium on porcine ICSI embryos expressing EGFP is related to activation of ooplasmic DNase I. Front. Agr. Sci. Eng. , 2015, 2(1): 84-89.

链接本文:

https://academic.hep.com.cn/fase/CN/10.15302/J-FASE-2015046
https://academic.hep.com.cn/fase/CN/Y2015/V2/I1/84

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