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Frontiers of Agricultural Science and Engineering

ISSN 2095-7505

ISSN 2095-977X(Online)

CN 10-1204/S

邮发代号 80-906

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Frontiers of Agricultural Science and Engineering  2015, Vol. 2 Issue (1): 60-65   https://doi.org/10.15302/J-FASE-2015048
  本期目录
Novel polymorphic EST-based microsatellite marker isolation and characterization from Poncirus trifoliata (Rutaceae)
Manosh Kumar BISWAS1,Peng CHEN1,Mohamed Hamdy AMAR1,2,Xiuxin DENG1,*()
1. Key Laboratory of Horticultural Plant Biology (MOE), National Center of Citrus Breeding, Huazhong Agricultural University, Wuhan 430070, China
2. Desert Research Center, Egyptian Deserts Gene Bank, North Sinai, Egypt
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Abstract：

Novel Poncirus trifoliata simple sequence repeat (SSR) markers were developed to evaluate their utility for genetic diversity and breeding studies of P. trifoliata and related species. A total of 108 primer pairs were characterized by PCR amplification experiments. Among these, 61 were polymorphic and transferable to other citrus species. The number of alleles per locus ranged from 2 to 6, with an average of 2.37 alleles per locus. The expected heterozygosity and observed heterozygosity ranged from 0 to 0.83 and 0 to 1.00, respectively. These novel polymorphic SSR markers will be useful for citrus cultivar identification and evaluation as well as breeding studies.
Key words： microsatellite    polymorphism    Poncirus trifoliata
收稿日期: 2015-02-11      出版日期: 2015-05-22
Corresponding Author(s): Xiuxin DENG   
 引用本文:   
. [J]. Frontiers of Agricultural Science and Engineering, 2015, 2(1): 60-65.
Manosh Kumar BISWAS,Peng CHEN,Mohamed Hamdy AMAR,Xiuxin DENG. Novel polymorphic EST-based microsatellite marker isolation and characterization from Poncirus trifoliata (Rutaceae). Front. Agr. Sci. Eng. , 2015, 2(1): 60-65.
 链接本文:  
https://academic.hep.com.cn/fase/CN/10.15302/J-FASE-2015048
https://academic.hep.com.cn/fase/CN/Y2015/V2/I1/60
Fig.1  
Fig.2  
Locus ID P. trifoliata (8) Fortunella (8) C. grandis (8)
AN He Ho PIC AN He Ho PIC AN He Ho PIC
PteSR046 2 0.48 0.40 0.36 1 0.00 0.00 0.00 1 0.00 0.00 0.00
PteSR666 4 0.64 0.80 0.58 2 0.50 1.00 0.38 4 0.69 1.00 0.63
PteSR168 2 0.50 1.00 0.38 2 0.38 0.50 0.30 2 0.38 0.00 0.30
PteSR581 2 0.50 1.00 0.38 2 0.50 1.00 0.38 2 0.22 0.25 0.19
PteSR484 2 0.48 0.40 0.36 2 0.50 1.00 0.38 1 0.00 0.00 0.00
PteSR661 2 0.50 0.60 0.38 1 0.00 0.00 0.00 2 0.38 0.50 0.30
PteSR563 3 0.46 0.60 0.41 2 0.50 1.00 0.38 2 0.22 0.25 0.19
PteSR755 2 0.28 0.33 0.24 2 0.50 1.00 0.38 3 0.66 0.75 0.58
PteSR299 2 0.48 0.40 0.36 1 0.00 0.00 0.00 3 0.66 0.75 0.58
PteSR550 3 0.63 0.50 0.55 1 0.00 0.00 0.00 2 0.50 1.00 0.38
PteSR493 4 0.58 0.60 0.54 2 0.50 1.00 0.38 2 0.50 1.00 0.38
PteSR366 4 0.48 0.60 0.45 2 0.50 1.00 0.38 2 0.38 0.00 0.30
Mean 2.667 0.501 0.603 0.416 1.667 0.323 0.625 0.247 2.167 0.383 0.458 0.319
Locus ID C. sinensis (5) C. reticulata (8) Lemone-Lime (5)
AN He Ho PIC AN He Ho PIC AN He Ho PIC
PteSR046 2 0.18 0.20 0.16 3 0.56 0.00 0.50 1 0.00 0.00 0.00
PteSR666 2 0.50 1.00 0.38 4 0.74 1.00 0.69 6 0.83 1.00 0.81
PteSR168 3 0.58 0.20 0.49 4 0.58 0.60 0.54 3 0.67 0.67 0.59
PteSR581 2 0.42 0.60 0.33 2 0.50 0.20 0.38 2 0.50 1.00 0.38
PteSR484 3 0.56 0.40 0.50 1 0.00 0.00 0.00 1 0.00 0.00 0.00
PteSR661 2 0.42 0.60 0.33 2 0.42 0.60 0.33 2 0.50 1.00 0.38
PteSR563 2 0.50 0.20 0.38 2 0.18 0.20 0.16 3 0.61 1.00 0.54
PteSR755 1 0.00 0.00 0.00 4 0.58 0.80 0.54 4 0.75 1.00 0.70
PteSR299 3 0.56 0.00 0.50 2 0.32 0.00 0.27 1 0.00 0.00 0.00
PteSR550 4 0.70 0.80 0.65 4 0.72 1.00 0.67 3 0.61 0.33 0.54
PteSR493 2 0.50 1.00 0.38 3 0.66 0.80 0.59 3 0.61 1.00 0.54
PteSR366 3 0.34 0.20 0.31 2 0.18 0.20 0.16 2 0.50 1.00 0.38
Mean 2.417 0.438 0.433 0.368 2.750 0.453 0.450 0.403 2.583 0.465 0.667 0.405
Tab.1  
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