Transcriptomic basis of neutrophil ratio variation induced by poly I:C stimulation in porcine peripheral blood

doi:10.15302/J-FASE-2017162

Frontiers of Agricultural Science and Engineering

2017, Vol. 4

Issue (3): 342-352 https://doi.org/10.15302/J-FASE-2017162

本期目录

Transcriptomic basis of neutrophil ratio variation induced by poly I:C stimulation in porcine peripheral blood

Haiyan WANG^1,^2,³, Qiaoxia ZHANG^1,², Lilin YIN^1,², Xiangdong LIU^1,², Shuhong ZHAO^1,², Mengjin ZHU^1,², Changchun LI^1,²(

)

¹. Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education & Key Laboratory of Swine Genetics and Breeding, Ministry of Agriculture, Huazhong Agricultural University, Wuhan 430070, China
². The Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural University, Wuhan 430070, China
³. College of Informatics, Huazhong Agricultural University, Wuhan 430070, China

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Abstract：

Neutrophils are vital components of defense mechanisms against invading pathogens and are closely linked with the individual antiviral capacity of pigs and other mammals. Neutrophilia is a well-known clinical characteristic of viral and bacterial infections. Using Affymetrix porcine genome microarrays, we investigated the gene expression profiles associated with neutrophil variation in porcine peripheral blood before and after polyriboinosinic-polyribocytidylic acid stimulation. Transcriptomic analysis showed 796 differentially expressed genes (DEGs) in extreme response (ER) pigs and 192 DEGs in moderate response (MR) pigs. Most DEGs were related to immune responses, included MXD1, CXCR4, CREG1, MyD88, CD14, TLR2, TLR4, IRF3 and IRF7. Gene ontology analysis indicated that the DEGs of both ER and MR pigs were involved in common biological processes, such as cell proliferation, growth regulation, immune response, inflammatory response and cell activation. The ER and MR groups also showed differences in DEGs involved in biological processes. DEGs involved in cell division and cell cycle were specifically found in the ER pigs, whereas DEGs involved in cell migration were specifically found in the MR pigs. The study provides a basic understanding of the molecular basis for the antiviral capacity of pigs and other mammals.

Key words： neutrophil peripheral blood pig poly I:C transcriptome

收稿日期: 2017-01-21 出版日期: 2017-09-12

Corresponding Author(s): Changchun LI

引用本文:

. [J]. Frontiers of Agricultural Science and Engineering, 2017, 4(3): 342-352.
Haiyan WANG, Qiaoxia ZHANG, Lilin YIN, Xiangdong LIU, Shuhong ZHAO, Mengjin ZHU, Changchun LI. Transcriptomic basis of neutrophil ratio variation induced by poly I:C stimulation in porcine peripheral blood. Front. Agr. Sci. Eng. , 2017, 4(3): 342-352.

链接本文:

https://academic.hep.com.cn/fase/CN/10.15302/J-FASE-2017162
https://academic.hep.com.cn/fase/CN/Y2017/V4/I3/342

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