Please wait a minute...
Frontiers of Agricultural Science and Engineering

ISSN 2095-7505

ISSN 2095-977X(Online)

CN 10-1204/S

邮发代号 80-906

Frontiers of Agricultural Science and Engineering  2021, Vol. 8 Issue (4): 635-644   https://doi.org/10.15302/J-FASE-2020343
  本期目录
IN VITRO ACTIVITY OF EXTRACTS OF FIVE MEDICINAL PLANT SPECIES ON PLANT PATHOGENIC FUNGI
Shufeng YAO1,2, Jiali XU1,2, Haibo ZHANG1,2, Hong GAO1,2, Shibin SHANG1,2(), Dan WANG1,2
1. Institute of Chemical Industry of Forest Products, Chinese Academy of Forestry, Key Laboratory of Biomass Energy and Material, Jiangsu Province, Key Laboratory of Chemical Engineering of Forest Products, National Forestry and Grassland Administration, Nanjing 210042, China.
2. Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, Nanjing Forestry University, Nanjing 210037, China.
 全文: PDF(1739 KB)   HTML
Abstract

• Ethyl acetate and 95% ethanol extracts from T. chinensis rhizomes gave great inhibition on six plant pathogenic fungi

T. chinensis has potential to be a new natural fungicide resource

T. chinensis has potential to be used for the preservation of agricultural and forestry products such as fruits and vegetables

The antifungal effectiveness of extracts of five medicinal plant species was determined. The inhibitory activity of extracts of Eucalyptus tereticornis, Xanthium sibiricum, Artemisia argyi, Tupistra chinensis and Pyrola calliantha were evaluated against the mycelial growth of the plant pathogenic fungi Aspergillus niger, Botrytis cinerea, Penicillium digitatum, P. expansum, P. italicum and Rhizopus stolonifer. All plant extracts were prepared at 60°C using solvents (either water, 50% ethanol (v/v), 95% ethanol (v/v), ethyl acetate or petroleum ether). Fungicidal effects of all plants tested were confirmed. Different extracts from the same plant species gave different degrees of inhibition. All aqueous extracts had weak or no activity on all fungi tested. Ethyl acetate and 95% ethanol extracts from T. chinensis rhizomes gave greater inhibition and a broader spectrum inhibition than the other extracts. T. chinensis may have potential as a new natural fungicide and may be used for the preservation of agricultural and forestry products such as fruits and vegetables.

Key wordsBotrytis cinerea    food preservation    plant diseases    plant pathogenic fungi    Tupistra chinensis
收稿日期: 2019-07-31      出版日期: 2021-11-19
Corresponding Author(s): Shibin SHANG   
 引用本文:   
. [J]. Frontiers of Agricultural Science and Engineering, 2021, 8(4): 635-644.
Shufeng YAO, Jiali XU, Haibo ZHANG, Hong GAO, Shibin SHANG, Dan WANG. IN VITRO ACTIVITY OF EXTRACTS OF FIVE MEDICINAL PLANT SPECIES ON PLANT PATHOGENIC FUNGI. Front. Agr. Sci. Eng. , 2021, 8(4): 635-644.
 链接本文:  
https://academic.hep.com.cn/fase/CN/10.15302/J-FASE-2020343
https://academic.hep.com.cn/fase/CN/Y2021/V8/I4/635
Fig.1  
Fig.2  
Crude extract Day 1 Day 2 Day 3 Day 4 Day 5
Water 5.50±0.28 >8.4
50% ethanol 2.75±0.05 5.45±0.30 >8.4
95% ethanol 0.62±0.07 1.08±0.19 1.50±0.25 1.63±0.25 2.07±0.31
Ethyl acetate 0.88±0.18 2.03±0.21 3.70±0.50 4.32±0.41 5.88±0.54
Petroleum ether 1.48±0.28 3.25±0.24 >8.4
Water control 5.35±0.17 >8.4
Carbendazim 5.17±0.45 >8.4
Tab.1  
Fig.3  
Fig.4  
Fig.5  
1 M Quaglia, C Moretti, M Cerri, G Linoci, G Cappelletti, S Urbani, A Taticchi. Effect of extracts of wastewater from olive milling in postharvest treatments of pomegranate fruit decay caused by Penicillium adametzioides. Postharvest Biology and Technology, 2016, 118: 26–34
https://doi.org/10.1016/j.postharvbio.2016.03.012
2 Y G Xing, Q L Xu, X H Li, Z M Che, J Yun. Antifungal activities of clove oil against Rhizopus Nigricans, Aspergillus Flavus and Penicillium Citrinumin vitro and in wounded fruit test. Journal of Food Safety, 2012, 32(1): 84–93
https://doi.org/10.1111/j.1745-4565.2011.00347.x
3 F D C O Freire, M E B da Rocha. Impact of mycotoxins on human health. In: Mérillon J M, Ramawat K, eds. Fungal Metabolites.Switzerland: Springer International Publishing,2016, 239–261
4 M E Zain. Impact of mycotoxins on humans and animals. Journal of Saudi Chemical Society, 2011, 15(2): 129–144
https://doi.org/10.1016/j.jscs.2010.06.006
5 R J Verma. Aflatoxin cause DNA damage. International Journal of Human Genetics, 2004, 4(4): 231–236
https://doi.org/10.1080/09723757.2004.11885899
6 S K Chhonker, D Rawat, R A Naik, R K Koiri. An overview of mycotoxins in human health with emphasis on development and progression of liver cancer. Clinical Oncology, 2018, 3: 1408
7 G Wichmann, O Herbarth, I Lehmann. The mycotoxins citrinin, gliotoxin, and patulin affect interferon-gamma rather than interleukin-4 production in human blood cells. Environmental Toxicology, 2002, 17(3): 211–218
https://doi.org/10.1002/tox.10050
8 A M Bozdogan. Assessment of total risk on non-target organisms in fungicide application for agricultural sustainability. Sustainability, 2014, 6(2): 1046–1058
https://doi.org/10.3390/su6021046
9 E Evans. Systemic fungicides in practice. Pest Management Science, 1971, 2(5): 192–196
https://doi.org/10.1002/ps.2780020503
10 R Vilaplana, L Pazmiño, S Valencia-Chamorro. Control of anthracnose, caused by Colletotrichum musae, on postharvest organic banana by thyme oil. Postharvest Biology and Technology, 2018, 138: 56–63
https://doi.org/10.1016/j.postharvbio.2017.12.008
11 D Zhou, Z Wang, M Li, M Xing, T Xian, K Tu. Carvacrol and eugenol effectively inhibit Rhizopus stolonifer and control postharvest soft rot decay in peaches. Journal of Applied Microbiology, 2018, 124(1): 166–178
https://doi.org/10.1111/jam.13612
12 G Simonetti, E Brasili, F D D′Auria, S Corpolongo, F Ferrari, G Pasqua, A. Valletta Prenylated flavonoids and total extracts from Morus nigra L. root bark inhibit in vitro growth of plant pathogenic fungi. Plant Biosystems, 2017, 151(5): 783–787
https://doi.org/10.1080/11263504.2017.1320313
13 L X Xia, T B Ng. Actinchinin, a novel antifungal protein from the gold kiwi fruit. Peptides, 2004, 25(7): 1093–1098
https://doi.org/10.1016/j.peptides.2004.05.002
14 F M Porsche, D Molitor, M Beyer, S Charton, C Andre, A Kollar. Antifungal activity of saponins from the fruit pericarp of Sapindus mukorossi against Venturia inaequalis and Botrytis cinerea. Plant Disease, 2018, 102(5): 991–1000
https://doi.org/10.1094/PDIS-06-17-0906-RE
15 G X Wu, A Y Liu, W X Chen. Effects of extracts of Tupistra chinensis on mycelia growth of Peronophythora litchii and storage life of litchi fruit. Scientia Agricultura Sinica, 2006, 39(8): 1703–1708 (in Chinese)
16 G X Wu, X L Yang, A Y Liu, W X Chen. Antifungal activity of Tupistra chinensis extract against Colletotrichum musae of postharvest banana. Transactions of the Chinese Society of Agricultural Engineering, 2007, 23(7): 235–240 (in Chinese)
17 G G Hasi, Q J Ai, Y Q Wei, Z Y Wu, X Zhang. Antifungal activity of Chinese herbal medicine extracts against two postharvest pathogenic fungi from vegetables. Journal of Beijing University of Agriculture, 2009, 24(1): 20–23 (in Chinese)
18 M Vinatoru, T J Mason, I Calinescu. Ultrasonically assisted extraction (UAE) and microwave assisted extraction (MAE) of functional compounds from plant materials. TrAC Trends in Analytical Chemistry, 2017, 97: 159–178
https://doi.org/10.1016/j.trac.2017.09.002
19 A Patel Divyakant, V C Darji, H Bariya Aditi, R Patel Kaushik, N Sonpal Rakshit. Evaluation of antifungal activity of Neolamarckia cadamba (roxb.) Bosser leaf and bark extract. International research journal of pharmacy, 2011, 2(5): 192–193
20 P F Souza Filho, R B Nair, D Andersson, P R Lennartsson, M J Taherzadeh. Vegan-mycoprotein concentrate from pea-processing industry byproduct using edible filamentous fungi. Fungal Biology and Biotechnology, 2018, 5(5): 1–10
https://doi.org/10.1186/s40694-018-0050-9“
21 Y D More, R M Gade, A V Shitole. Evaluation of antifungal activities of extracts of Aegle marmelos, Syzygium cumini and Pongamia pinnata against Pythium debaryanum. Indian Journal of Pharmaceutical Sciences, 2017, 79(3): 377–384
https://doi.org/10.4172/pharmaceutical-sciences.1000240
22 M A Hossain, K A S Al-Raqmi, Z H Al-Mijizy, A M Weli, Q Al-Riyami. Study of total phenol, flavonoids contents and phytochemical screening of various leaves crude extracts of locally grown Thymus vulgaris. Asian Pacific Journal of Tropical Biomedicine, 2013, 3(9): 705–710
https://doi.org/10.1016/S2221-1691(13)60142-2
23 A J Afolayan, D S Grierson, L Kambizi, I Madamombe, P J Masika, A K Jäger. In vitro antifungal activity of some South African medicinal plants. South African Journal of Botany, 2002, 68(1): 72–76
https://doi.org/10.1016/S0254-6299(15)30444-0
24 K M Thembo, H F Vismer, N Z Nyazema, W C A Gelderblom, D R Katerere. Antifungal activity of four weedy plant extracts against selected mycotoxigenic fungi. Journal of Applied Microbiology, 2010, 109(4): 1479–1486
https://doi.org/10.1111/j.1365-2672.2010.04776.x
25 A Sen, A Batra. Evaluation of antimicrobial activity of different solvent extracts of medicinal plant: Melia azedarach L. International Journal of Current Pharmaceutical Research, 2012, 4(2): 67–73
26 J Bakht, M Tayyab, H Ali, A Islam, M Shafi. Effect of different solvent extracted sample of Allium sativum (Linn) on bacteria and fungi. African Journal of Biotechnology, 2011, 10(31): 5910–5915
27 A Ten Have, W Mulder, J Visser, J A Van Kan. The endopolygalacturonase gene Bcpg1 is required for full virulence of Botrytis cinerea. American Phytopathological Society, 1998, 11(10): 1009–1016
28 H J Rosslenbroich, D Stuebler. Botrytis cinerea history of chemical control and novel fungicides for its management. Crop Protection, 2000, 19(8–10): 557–561
https://doi.org/10.1016/S0261-2194(00)00072-7
29 B Williamson, B Tudzynski, P Tudzynski, J A Van Kan. Botrytis cinerea: the cause of grey mould disease. Molecular Plant Pathology, 2007, 8(5): 561–580
https://doi.org/10.1111/j.1364-3703.2007.00417.x
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed