Bioproduction of D -psicose using permeabilized cells of newly isolated Rhodobacter sphaeroides SK011
Bioproduction of D -psicose using permeabilized cells of newly isolated Rhodobacter sphaeroides SK011
Longtao ZHANG1,Bo JIANG2,Wanmeng MU2,Tao ZHANG2,
1.State Key Laboratory
of Food Science and Technology, Jiangnan University, Wuxi 214122,
China;Institute of Animal Husbandry
and Veterinary Medicine, Fujian Academy of Agricultural Sciences,
Fuzhou 350013, China; 2.State Key Laboratory
of Food Science and Technology, Jiangnan University, Wuxi 214122,
China;
Abstract:D-psicose exits in an extremely small amount in nature and is difficult to be chemically synthesized. Only three bacteria have been used in the biotransformation of D-psicose from D-fructose. In this paper, another bacterium which could convert D-fructose to D-psicose was isolated and identified as Rhodobacter sphaeroides. The process parameters of D-psicose production using permeabilized cells of Rhodobacter sphaeroides SK011 were optimized, including the permeabilization procedure: 0.1% (w/v) CTAB, 10min, and reaction conditions: cell concentration, 30g dry cell wt/L; concentration of substrate, 50g/L; 40°C, pH 9.0; reaction time, 8 h. Under the optimized conditions, the permeabilized cells produced approximately 6.5g/L D-psicose with a D-psicose productivity of 0.82g·L−1·h−1. This is the first report of bioproduction of D-psicose using permeabilized cells of Rhodobacter sphaeroides.
出版日期: 2009-12-05
引用本文:
. Bioproduction of D -psicose using permeabilized cells of newly isolated Rhodobacter sphaeroides SK011[J]. Front. Chem. Sci. Eng., 2009, 3(4): 393-398.
Longtao ZHANG, Bo JIANG, Wanmeng MU, Tao ZHANG, . Bioproduction of D -psicose using permeabilized cells of newly isolated Rhodobacter sphaeroides SK011. Front. Chem. Sci. Eng., 2009, 3(4): 393-398.
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