Evolutionary engineering of Phaffia rhodozyma for astaxanthin-overproducing strain
Evolutionary engineering of Phaffia rhodozyma for astaxanthin-overproducing strain
Jixian GONG1,2(), Nan DUAN2, Xueming ZHAO2
1. School of Textiles, Tianjin Polytechnic University, Tianjin 300160, China; 2. School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China
Evolutionary engineering is a novel whole-genome wide engineering strategy inspired by natural evolution for strain improvement. Astaxanthin has been widely used in cosmetics, pharmaceutical and health care food due to its capability of quenching active oxygen. Strain improvement of Phaffia rhodozyma, one of the main sources for natural astaxanthin, is of commercial interest for astaxanthin production. In this study a selection procedure was developed for adaptive evolution of P. rhodozyma strains under endogenetic selective pressure induced by additive in environmental niches. Six agents, which can induce active oxygen in cells, were added to the culture medium respectively to produce selective pressure in process of evolution. The initial strain, P. rhodozyma AS2-1557, was mutagenized to acquire the initial strain population, which was then cultivated for 550 h at selective pressure and the culture was transferred every 48h. Finally, six evolved strains were selected after 150 generations of evolution. The evolved strains produced up to 48.2% more astaxanthin than the initial strain. Our procedure may provide a promising alternative for improvement of high-production strain.
Corresponding Author(s):
GONG Jixian,Email:gongjixian@126.com
引用本文:
. Evolutionary engineering of Phaffia rhodozyma for astaxanthin-overproducing strain[J]. Frontiers of Chemical Science and Engineering, 2012, 6(2): 174-178.
Jixian GONG, Nan DUAN, Xueming ZHAO. Evolutionary engineering of Phaffia rhodozyma for astaxanthin-overproducing strain. Front Chem Sci Eng, 2012, 6(2): 174-178.
Selifonova O, Valle F, Schellenberger V. Rapid evolution of novel traits in microorganisms. Applied and Environmental Microbiology , 2001, 67(8): 3645-3649 doi: 10.1128/AEM.67.8.3645-3649.2001
2
Sonderegger M, Sauer U. Evolutionary engineering of Saccharomyces cerevisiae for anaerobic growth on xylose. Applied and Environmental Microbiology , 2003, 69(4): 1990-1998 doi: 10.1128/AEM.69.4.1990-1998.2003
3
Steiner P, Sauer U. Long-term continuous evolution of acetate resistant Acetobacter aceti. Biotechnology and Bioengineering , 2003, 84(1): 40-44 doi: 10.1002/bit.10741
4
van Maris A J A, Geertman J M A, Vermeulen A, Groothuizen M K, Winkler A A, Piper M D W, van Dijken J P, Pronk J T. Directed evolution of pyruvate decarboxylase-negative Saccharomyces cerevisiae, yielding a C-2-independent, glucose-tolerant, and pyruvate-hyperproducing yeast. Applied and Environmental Microbiology , 2004, 70(1): 159-166 doi: 10.1128/AEM.70.1.159-166.2004
5
Cakar Z P, Seker U O S, Tamerler C, Sonderegger M, Sauer U. Evolutionary engineering of multiple-stress resistant Saccharomyces cerevisiae. FEMS Yeast Research , 2005, 5(6-7): 569-578 doi: 10.1016/j.femsyr.2004.10.010
6
Kuyper M, Toirkens M, Diderich J, Winkler A, Vandijken J, Pronk J. Evolutionary engineering of mixed-sugar utilization by a xylose-fermenting Saccharomyces cerevisiae strain. FEMS Yeast Research , 2005, 5: 925-934 doi: 10.1016/j.femsyr.2005.04.004
7
Wisselink H W, Toirkens M J, del Rosario Franco Berriel M, Winkler A A, van Dijken J P, Pronk J T, van Maris A J A. Engineering of Saccharomyces cerevisiae for efficient anaerobic alcoholic fermentation of L-arabinose. Applied and Environmental Microbiology , 2007, 73(15): 4881-4891 doi: 10.1128/AEM.00177-07
8
Guimaraes P M R, Francois J, Parrou J L, Teixeira J A, Domingues L. Adaptive evolution of a lactose-consuming Saccharomyces cerevisiae recombinant. Applied and Environmental Microbiology , 2008, 74(6): 1748-1756 doi: 10.1128/AEM.00186-08
9
Jantama K, Haupt M J, Svoronos S A, Zhang X, Moore J C, Shanmugam K T, Ingram L O. Combining metabolic engineering and metabolic evolution to develop nonrecombinant strains of Escherichia coli C that produce succinate and malate. Biotechnology and Bioengineering , 2008, 99(5): 1140-1153 doi: 10.1002/bit.21694
10
Meijnen J P, de Winde J H, Ruijssenaars H J. Engineering pseudomonas putida S12 for efficient utilization of D-xylose and L-arabinose. Applied and Environmental Microbiology , 2008, 74(16): 5031-5037 doi: 10.1128/AEM.00924-08
11
Cakar Z P, Alk?m C, Turanl? B, Tokman N, Akman S, Sar?kaya M, Tamerler C, Benbadis L, Fran?ois J M. Isolation of cobalt hyper-resistant mutants of Saccharomyces cerevisiae by in vivo evolutionary engineering approach. Journal of Biotechnology , 2009, 143(2): 130-138 doi: 10.1016/j.jbiotec.2009.06.024
12
Gilbert A, Sangurdekar D P, Srienc F. Rapid strain improvement through optimized evolution in the cytostat. Biotechnology and Bioengineering , 2009, 103(3): 500-512 doi: 10.1002/bit.22272
13
Wisselink H W, Toirkens M J, Wu Q, Pronk J T, Maris A JA. Novel evolutionary engineering approach for accelerated utilization of glucose, xylose, and arabinose mixtures by engineered Saccharomyces cerevisiae strains. Applied and Environmental Microbiology , 2009, 75(4): 907-914 doi: 10.1128/AEM.02268-08
