Uptake and accumulation of multiwalled carbon nanotubes change the morphometric and biochemical characteristics of Onobrychis arenaria seedlings
Uptake and accumulation of multiwalled carbon nanotubes change the morphometric and biochemical characteristics of Onobrychis arenaria seedlings
Elena SMIRNOVA1(), Alexander GUSEV2, Olga ZAYTSEVA2, Olga SHEINA2, Alexey TKACHEV3, Elena KUZNETSOVA4, Elena LAZAREVA1, Galina ONISHCHENKO1, Alexey FEOFANOV1,5, Mikhail KIRPICHNIKOV1,5
1. Biology Faculty, Lomonosov Moscow State University, Moscow 119991, Russia; 2. Derzhavin Tambov State University, Tambov 392000, Russia; 3. Siberian Institute of Plants Physiology and Biochemistry, Siberian Branch, Russian Academy of Sciences, Irkutsk 664033, Russia; 4. NanoTechCenter Ltd., Tambov 392000, Russia; 5. Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow 117997, Russia
We have studied the effect of the engineered nanomaterial Taunit, containing multiwalled carbon nanotubes (MWCNTs), on the growth of Onobrychis arenaria seedlings and investigated whether affected plants uptake and accumulate MWCNTs. We found that 100 μg/mL and 1000 μg/mL of Taunit stimulated the growth of roots and stems, and enhanced the peroxidase activity in these parts of plants. Microscopy studies showed the presence of MWCNTs in the root and leaf tissues of seedlings exposed to Taunit, suggesting that MWCNTs have a capacity to penetrate the cell walls, accumulate in roots and translocate to the leaves. Thus the stimulating effect of MWCNTs on seedlings of O. arenaria may be associated with the primary uptake and accumulation of MWCNTs by plant roots followed by translocation to the other plant tissues.
. Uptake and accumulation of multiwalled carbon nanotubes change the morphometric and biochemical characteristics of Onobrychis arenaria seedlings[J]. Frontiers of Chemical Science and Engineering, 2012, 6(2): 132-138.
Elena SMIRNOVA, Alexander GUSEV, Olga ZAYTSEVA, Olga SHEINA, Alexey TKACHEV, Elena KUZNETSOVA, Elena LAZAREVA, Galina ONISHCHENKO, Alexey FEOFANOV, Mikhail KIRPICHNIKOV. Uptake and accumulation of multiwalled carbon nanotubes change the morphometric and biochemical characteristics of Onobrychis arenaria seedlings. Front Chem Sci Eng, 2012, 6(2): 132-138.
Peroxidase activity /(arbitrary units of activity/gram of tissue fresh weight/second)
1000 mg/L
114
102
25.4±1.28
32±0.97
0.185±0.019
100 mg/L
114
107
25.7±1.01
28.7±0.83
0.313±0.012
ControL
100
100
13.92±1.28
18.4±1.28
0.115±0.012
Tab.1
Concentrations
Stems /mm
Roots /mm
10 mg/L
17.3±1.21
31.3±0.9
1 mg/L
19.6±0.90
29.5±1.2
0.1 mg/L
19.0±0.81
25.6±1.21
Control
13.2±0.71
25.4±1.4
Tab.2
Fig.1
Fig.2
Fig.3
Fig.4
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