Nano-hydroxyapatite formation via co-precipitation with chitosan-g-poly(N-isopropylacrylamide) in coil and globule states for tissue engineering application
Nano-hydroxyapatite formation via co-precipitation with chitosan-g-poly(N-isopropylacrylamide) in coil and globule states for tissue engineering application
Yang YU1, Hong ZHANG2(), Hong SUN3, Dandan XING1, Fanglian YAO1()
1. School of Chemical Engineering and Technology, Key Laboratory of Systems Bioengineering of Ministry of Education, Tianjin University, Tianjin 300072, China; 2. Consolidated Research Institute for Advanced Science and Medical Care, Waseda University (ASMeW), Shinjuku-ku, Tokyo 162-0041, Japan; 3. Basic Medical College, Hebei United University, Tangshan 063000, China
With the excellent biocompatibility and osteoconductivity, nano-hydroxyapatite (nHA) has shown significant prospect in the biomedical applications. Controlling the size, crystallinity and surface properties of nHA crystals is a critical challenge in the design of HA based biomaterials. With the graft copolymer of chitosan and poly(N-isopropylacrylamide) in coil and globule states as a template respectively, a novel composite from chitosan-g-poly(N-isopropylacrylamide) and nano-hydroxyapatite (CS-g-PNIPAM/nHA) was prepared via co-precipitation. Zeta potential analysis, thermogravimetric analysis and X-ray diffraction were used to identify the formation mechanism of the CS-g-PNIPAM/nHA composite and its morphology was observed by transmission electron microscopy. The results suggested that the physical aggregation states of the template polymer could induce or control the size, crystallinity and morphology of HA crystals in the CS-g-PNIPAM/nHA composite. The CS-g-PNIPAM/nHA composite was then introduced to chitosan-gelatin (CS-Gel) polyelectronic complex and the cytocompatibility of the resulting CS-Gel/composite hybrid film was evaluated. This hybrid film was proved to be favorable for the proliferation of MC 3T3-E1 cells. Therefore, the CS-g-PNIPAM/nHA composite is a potential biomaterial in bone tissue engineering.
Corresponding Author(s):
ZHANG Hong,Email:yaofanglian@tju.edu.cn; YAO Fanglian,Email:hzhang@aoni.waseda.jp
引用本文:
. Nano-hydroxyapatite formation via co-precipitation with chitosan-g-poly(N-isopropylacrylamide) in coil and globule states for tissue engineering application[J]. Frontiers of Chemical Science and Engineering, 2013, 7(4): 388-400.
Yang YU, Hong ZHANG, Hong SUN, Dandan XING, Fanglian YAO. Nano-hydroxyapatite formation via co-precipitation with chitosan-g-poly(N-isopropylacrylamide) in coil and globule states for tissue engineering application. Front Chem Sci Eng, 2013, 7(4): 388-400.
Zhou H, Lee J. Nanoscale hydroxyapatite particles for bone tissue engineering. Acta Biomaterialia , 2011, 7(7): 2769–2781 doi: 10.1016/j.actbio.2011.03.019
2
Thanigaiarul K, Elayaraja K, Magudapathy P, Kamachi Mudali U, Nair K G M, Sudarshan M, Krishna J B M, Chakraborty A, Narayana Kalkura S. Surface modification of nanocrystalline calcium phosphate bioceramic by low energy nitrogen ion implantation. Ceramics International , 2013, 39(3): 3027–3034 doi: 10.1016/j.ceramint.2012.09.081
3
Pang Y X, Bao X. Influence of temperature, ripening time and calcination on the morphology and crystallinity of hydroxyapatite nanoparticles. Journal of the European Ceramic Society , 2003, 23(10): 1697–1704 doi: 10.1016/S0955-2219(02)00413-2
4
Shen X Y, Chen L, Cai X A, Tong T, Tong H, Hu J M. A novel method for the fabrication of homogeneous hydroxyapatite/collagen nanocomposite and nanocomposite scaffold with hierarchical porosity. Journal of Materials Science. Materials in Medicine , 2011, 22(2): 299–305 doi: 10.1007/s10856-010-4199-x
5
Jin H H, Kim D H, Kim T W, Shin K K, Jung J S, Park H C, Yoon S Y. In vivo evaluation of porous hydroxyapatite/chitosan-alginate composite scaffolds for bone tissue engineering. International Journal of Biological Macromolecules , 2012, 51(5): 1079–1085 doi: 10.1016/j.ijbiomac.2012.08.027
6
Li J J, Zhu D W, Yin J W, Liu Y X, Yao F L, Yao K D. Formation of nano-hydroxyapatite crystal in situ in chitosan-pectin polyelectrolyte complex network. Materials Science & Engineering C-Materials for Biological Applications , 2010, 30(6): 795–803 doi: 10.1016/j.msec.2010.03.011
7
Dai Y F, Xu M, Wei J C, Zhang H B, Chen Y W. Surface modification of hydroxyapatite nanoparticles by poly(L-phenylalanine) via ROP of L-phenylalanine N-carboxyanhydride (Pha-NCA). Applied Surface Science , 2012, 258(7): 2850–2855 doi: 10.1016/j.apsusc.2011.10.147
8
Poursamar S A, Azami M, Mozafari M. Controllable synthesis and characterization of porous polyvinyl alcohol/hydroxyapatite nanocomposite scaffolds via an in situ colloidal technique. Colloids and Surfaces. B, Biointerfaces , 2011, 84(2): 310–316 doi: 10.1016/j.colsurfb.2011.01.015
9
Kailasanathan C, Selvakumar N. Comparative study of hydroxyapatite/gelatin composites reinforced with bio-inert ceramic particles. Ceramics International , 2012, 38(5): 3569–3582 doi: 10.1016/j.ceramint.2011.12.073
10
Chang M C, Ko C C, Douglas W H. Conformational change of hydroxyapatite/gelatin nanocomposite by glutaraldehyde. Biomaterials , 2003, 24(18): 3087–3094 doi: 10.1016/S0142-9612(03)00150-9
11
Chang M C, Ko C C, Douglas W H. Preparation of hydroxyapatite-gelatin nanocomposite. Biomaterials , 2003, 24(17): 2853–2862 doi: 10.1016/S0142-9612(03)00115-7
12
Liou S C, Chen S Y, Liu D M. Synthesis and characterization of needlelike apatitic nanocomposite with controlled aspect ratios. Biomaterials , 2003, 24(22): 3981–3988 doi: 10.1016/S0142-9612(03)00303-X
13
Spanos N, Deimede V, Koutsoukos P G. Functionalization of synthetic polymers for potential use as biomaterials: selective growth of hydroxyapatite on sulphonated polysulphone. Biomaterials , 2002, 23(3): 947–953 doi: 10.1016/S0142-9612(01)00207-1
14
Tien W B, Chen M T, Yao P C. Effects of pH and temperature on microstructure and morphology of hydroxyapatite/collagen composites synthesized in vitro. Materials Science & Engineering C-Materials for Biological Applications , 2012, 32(7): 2096–2102 doi: 10.1016/j.msec.2012.05.046
15
Sena L A, Caraballo M M, Rossi A M, Soares G A. Synthesis and characterization of biocomposites with different hydroxyapatite-collagen ratios. Journal of Materials Science. Materials in Medicine , 2009, 20(12): 2395–2400 doi: 10.1007/s10856-009-3813-2
16
Victor S P, Sharma C P. Development and evaluation of cyclodextrin complexed hydroxyapatite nanoparticles for preferential albumin adsorption. Colloids and Surfaces. B, Biointerfaces , 2011, 85(2): 221–228 doi: 10.1016/j.colsurfb.2011.02.032
17
Xiao X F, Liu R F, Qiu C F, Zhu D C, Liu F. Biomimetic synthesis of micrometer spherical hydroxyapatite with beta-cyclodextrin as template. Materials Science & Engineering C–Biomimetic and Supramolecular Systems , 2009, 29(3): 785–790 doi: 10.1016/j.msec.2008.07.015
18
Chen J D, Nan K H, Yin S H, Wang Y J, Wu T, Zhang Q Q. Characterization and biocompatibility of nanohybrid scaffold prepared via in situ crystallization of hydroxyapatite in chitosan matrix. Colloids and Surfaces. B, Biointerfaces , 2010, 81(2): 640–647 doi: 10.1016/j.colsurfb.2010.08.017
19
Rusu V M, Ng C H, Wilke M, Tiersch B, Fratzl P, Peter M G. Size-controlled hydroxyapatite nanoparticles as self-organized organic-in organic composite materials. Biomaterials , 2005, 26(26): 5414–5426 doi: 10.1016/j.biomaterials.2005.01.051
20
Wilson O C Jr, Hull J R. Surface modification of nanophase hydroxyapatite with chitosan. Materials Science & Engineering C-Biomimetic and Supramolecular Systems , 2008, 28(3): 434–437 doi: 10.1016/j.msec.2007.04.005
21
Kim B-S. Yong S C, Sin Y-W, Ryu K-H, Lee J, You H-K. Growth and osteogenic differentiation of alveolar human bone marrow-derived mesenchymal stem cells on chitosan/hydroxyapatite composite fabric. Journal of Biomedical Materials Research. Part A , 2013, 101A(6): 1550–1558 doi: 10.1002/jbm.a.34456
22
Davidenko N, Carrodeguas R G, Peniche C, Solis Y, Cameron R E. Chitosan/apatite composite beads prepared by in situ generation of apatite or Si-apatite nanocrystals. Acta Biomaterialia , 2010, 6(2): 466–476 doi: 10.1016/j.actbio.2009.07.029
23
He D, Xiao X F, Liu F, Liu R F. Chondroitin sulfate template-mediated biomimetic synthesis of nano-flake hydroxyapatite. Applied Surface Science , 2008, 255(2): 361–364 doi: 10.1016/j.apsusc.2008.06.151
24
Lai J T, Filla D, Shea R. Functional polymers from novel carboxyl-terminated trithiocarbonates as highly efficient RAFT agents. Macromolecules , 2002, 35(18): 6754–6756 doi: 10.1021/ma020362m
25
Jiang J, Pan X D, Cao J X, Jiang J L, Hua D B, Zhu X L. Synthesis and property of chitosan graft copolymer by RAFT polymerization with tosylic acid-chitosan complex. International Journal of Biological Macromolecules , 2012, 50(3): 586–590 doi: 10.1016/j.ijbiomac.2012.01.033
26
Li J J, Chen Y P, Yin Y J, Yao F L, Yao K D. Modulation of nano-hydroxyapatite size via formation on chitosan-gelatin network film in situ. Biomaterials , 2007, 28(5): 781–790 doi: 10.1016/j.biomaterials.2006.09.042
27
Ho K M, Li W Y, Wong C H, Li P. Amphiphilic polymeric particles with core-shell nanostructures: emulsion-based syntheses and potential applications. Colloid & Polymer Science , 2010, 288(16–17): 1503–1523 doi: 10.1007/s00396-010-2276-9
28
Thompson C J, Ding C, Qu X, Yang Z, Uchegbu I F, Tetley L, Cheng W P. Uchegbu ljeoma F, Tetley L, Cheng W P. The effect of polymer architecture on the nano self-assemblies based on novel comb-shaped amphiphilic poly(allylamine). Colloid & Polymer Science , 2008, 286(13): 1511–1526 doi: 10.1007/s00396-008-1925-8
29
Chen C, Liu M, Gao C, Lü S, Chen J, Yu X, Ding E, Yu C, Guo J, Cui G. A convenient way to synthesize comb-shaped chitosan-graft-poly (N-isopropylacrylamide) copolymer. Carbohydrate Polymers , 2013, 92(1): 621–628 doi: 10.1016/j.carbpol.2012.09.014
30
Yang L R, Guo C, Jia L W, Xie K, Shou Q H, Liu H Z. Fabrication of Biocompatible Temperature- and pH-Responsive Magnetic Nanoparticles and Their Reversible Agglomeration in Aqueous Milieu. Industrial & Engineering Chemistry Research , 2010, 49(18): 8518–8525 doi: 10.1021/ie100587e
31
Landi E, Tampieri A, Celotti G, Sprio S. Densification behaviour and mechanisms of synthetic hydroxyapatites. Journal of the European Ceramic Society , 2000, 20(14–15): 2377–2387 doi: 10.1016/S0955-2219(00)00154-0
32
Hartgerink J D, Beniash E, Stupp S I. Self-assembly and mineralization of peptide-amphiphile nanofibers. Science , 2001, 294(5547): 1684–1688 doi: 10.1126/science.1063187
33
Li D, Newton S M C, Klebba P E, Mao C B. Flagellar Display of Bone-Protein-Derived Peptides for Studying Peptide-Mediated Biomineralization. Langmuir , 2012, 28(47): 16338–16346 doi: 10.1021/la303237u
34
Kandori K, Oda S, Fukusumi M, Morisada Y. Synthesis of positively charged calcium hydroxyapatite nano-crystals and their adsorption behavior of proteins. Colloids and Surfaces. B, Biointerfaces , 2009, 73(1): 140–145 doi: 10.1016/j.colsurfb.2009.05.011
35
Li J, Dou Y, Yang J, Yin Y, Zhang H, Yao F, Wang H, Yao K. Surface characterization and biocompatibility of micro- and nano-hydroxyapatite / chitosan-gelatin network films. Materials Science & Engineering C-Biomimetic and Supramolecular Systems , 2009, 29(4): 1207–1215 doi: 10.1016/j.msec.2008.09.038
36
Liu H F, Yin Y J, Yao K D. Construction of chitosan-gelatin-hyaluronic acid artificial skin in vitro. Journal of Biomaterials Applications , 2007, 21(4): 413–430
37
Lieb E, Tessmar J, Hacker M, Fischbach C, Rose D, Blunk T, Mikos A G, G?pferich A, Schulz M B. Poly(D,L-lactic acid)-poly(ethylene glycol)-monomethyl ether diblock copolymers control adhesion and osteoblastic differentiation of marrow stromal cells. Tissue Engineering , 2003, 9(1): 71–84 doi: 10.1089/107632703762687555
38
Li J J, Sun H, Sun D, Yao Y L, Yao F L, Yao K D. Biomimetic multicomponent polysaccharide/nano-hydroxyapatite omposites for bone tissue engineering. Carbohydrate Polymers , 2011, 85(4): 885–894
