Profiling influences of gene overexpression on heterologous resveratrol production in Saccharomyces cerevisiae

doi:10.1007/s11705-016-1601-3

Frontiers of Chemical Science and Engineering

2017, Vol. 11

Issue (1): 117-125 https://doi.org/10.1007/s11705-016-1601-3

本期目录

Profiling influences of gene overexpression on heterologous resveratrol production in Saccharomyces cerevisiae

Duo Liu^1,²,Bingzhi Li^1,²,Hong Liu^1,²,Xuejiao Guo^1,²,Yingjin Yuan^1,²(

)

¹. Key Laboratory of Systems Bioengineering (Ministry of Education), School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China
². SynBio Research Platform, Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Tianjin University, Tianjin 300072, China

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Abstract：

Metabolic engineering of heterologous resveratrol production in Saccharomyces cerevisiae faces challenges as the precursor L-tyrosine is stringently regulated by a complex biosynthetic system. We overexpressed the main gene targets in the upstream pathways to investigate their influences on the downstream resveratrol production. Single-gene overexpression and DNA assembly-directed multigene overexpression affect the production of resveratrol as well as its precursor p-coumaric acid. Finally, the collaboration of selected gene targets leads to an optimal resveratrol production of 66.14±3.74 mg·L^–1, 2.27 times higher than the initial production in YPD medium (4% glucose). The newly discovered gene targets TRP1 expressing phosphoribosylanthranilate isomerase, ARO3 expressing 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase, and 4CL expressing 4-coumaryl-CoA ligase show notable positive impacts on resveratrol production in S. cerevisiae.

Key words： resveratrol aromatic amino acid DNA assembly metabolic engineering gene overexpression

收稿日期: 2016-06-25 出版日期: 2017-03-17

Corresponding Author(s): Yingjin Yuan

引用本文:

. [J]. Frontiers of Chemical Science and Engineering, 2017, 11(1): 117-125.
Duo Liu,Bingzhi Li,Hong Liu,Xuejiao Guo,Yingjin Yuan. Profiling influences of gene overexpression on heterologous resveratrol production in Saccharomyces cerevisiae. Front. Chem. Sci. Eng., 2017, 11(1): 117-125.

链接本文:

https://academic.hep.com.cn/fcse/CN/10.1007/s11705-016-1601-3
https://academic.hep.com.cn/fcse/CN/Y2017/V11/I1/117

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