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Frontiers of Chemical Science and Engineering

ISSN 2095-0179

ISSN 2095-0187(Online)

CN 11-5981/TQ

邮发代号 80-969

2019 Impact Factor: 3.552

Frontiers of Chemical Science and Engineering  2019, Vol. 13 Issue (1): 140-151   https://doi.org/10.1007/s11705-018-1714-y
  本期目录
Detoxification and concentration of corn stover hydrolysate and its fermentation for ethanol production
Qing Li1, Yingjie Qin1,2(), Yunfei Liu1, Jianjun Liu1, Qing Liu1, Pingli Li1, Liqiang Liu2
1. School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China
2. Chembrane Engineering & Technology, Inc., Tianjin 300308, China
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Abstract

Environmental and energy concerns have increased interest in renewable energy sources, particularly biofuels. Thus the fermentation of glucose from sulfuric acid-hydrolyzed corn stover for the production of bioethanol has been explored using a combined acid retardation and continuous-effect membrane distillation treatment process. This process resulted in the separation of the sugars and acids from the acid-catalyzed hydrolysate, the removal of most of the fermentation inhibitors from the hydrolysate and the concentration of the detoxified hydrolysate. The recovery rate of glucose from the sugar-acid mixture using acid retardation was greater than 99.12% and the sulfuric acid was completely recovered from the hydrolysate. When the treated corn stover hydrolysate, containing 100 g/L glucose, was used as a carbon source, 43.06 g/L of ethanol was produced with a productivity of 1.79 g/(L∙h) and a yield of 86.31%. In the control experiment, where glucose was used as the carbon source these values were 1.97 g/(L∙h) and 93.10% respectively. Thus the integration of acid retardation and a continuous-effect membrane distillation process are effective for the production of fuel ethanol from corn stover.

Key wordscorn stover    hydrolysate    acid retardation    continuous-effect membrane distillation    ethanol fermentation
收稿日期: 2017-11-17      出版日期: 2019-02-25
Corresponding Author(s): Yingjie Qin   
 引用本文:   
. [J]. Frontiers of Chemical Science and Engineering, 2019, 13(1): 140-151.
Qing Li, Yingjie Qin, Yunfei Liu, Jianjun Liu, Qing Liu, Pingli Li, Liqiang Liu. Detoxification and concentration of corn stover hydrolysate and its fermentation for ethanol production. Front. Chem. Sci. Eng., 2019, 13(1): 140-151.
 链接本文:  
https://academic.hep.com.cn/fcse/CN/10.1007/s11705-018-1714-y
https://academic.hep.com.cn/fcse/CN/Y2019/V13/I1/140
Fig.1  
Fig.2  
Feed Glucose /(g?L−1) Sulfuric acid /wt-% R Rs /% Ra /%
Feed Eluent Feed Eluent
CSH 38.48 20.96 20.00 4.98 1.56 99.12 98.92
GSS 38.48 21.93 20.00 5.16 1.58 99.16 99.01
Tab.1  
Fig.3  
Substance Non-detoxified CSH /(g?L−1) Sugar eluent /(g?L−1) Removal rate /% Concentrated sugar solution /(g?L−1)
Glucose 38.479 20.956 NAa) 103.215
Total organic acids 4.678 NDb) 100 ND
Formic acid 3.483 ND 100 ND
Acetic acid 0.310 ND 100 ND
Levulinic acid 0.885 ND 100 ND
Furfural 0.003 ND 100 ND
5-HMF 0.164 ND 100 ND
Phenols 0.097 0.004 92.495 0.019
Sugar yield (%) NA 99.124 NA 100
Tab.2  
Fig.4  
Fig.5  
Substance Acid eluent /(g?L−1) Concentrated acid solution /(g?L−1) Highly concentrated acid solution /(g?L−1)
Formic acid 0.867 0.966 0.210
Acetic acid 0.077 0.064 0.016
Levulinic acid 0.220 0.874 0.105
Furfural ND 0.003 ND
5-HMF 0.041 0.059 0.012
Phenols 0.022 0.187 0.015
Tab.3  
Fig.6  
Fig.7  
No. Temperature A Inoculum size B Agitation rate C pH D Ethanol yield /%
1 1 (25 °C) 1 (5%) 1 (125 r/min) 1 (4.5) 75.232
2 1 (25 °C) 2 (10%) 2 (150 r/min) 2 (5.0) 86.201
3 1 (25 °C) 3 (15%) 3 (175 r/min) 3 (5.5) 83.434
4 2 (30 °C) 1 (5%) 3 (175 r/min) 2 (5.0) 91.943
5 2 (30 °C) 2 (10%) 1 (125 r/min) 3 (5.5) 90.566
6 2 (30 °C) 3 (15%) 2 (150 r/min) 1 (4.5) 92.611
7 3 (35 °C) 1 (5%) 2 (150 r/min) 3 (5.5) 81.931
8 3 (35 °C) 2 (10%) 3 (175 r/min) 1 (4.5) 86.810
9 3 (35 °C) 3 (15%) 1 (125 r/min) 2 (5.0) 86.723
K1 244.867 249.107 252.521 254.654 NA
K2 275.121 263.577 260.743 264.868 NA
K3 255.465 262.768 262.188 255.931 NA
k1 81.622 83.036 84.174 84.885 NA
k2 91.707 87.859 86.914 88.289 NA
k3 85.155 87.590 87.399 85.310 NA
R 10.085 4.824 3.222 3.405 NA
Q A2 B2 C3 D2 NA
Tab.4  
Experiment Initial glucose /(g?L−1) Residual glucose /(g?L−1) Ethanol concentration/(g?L−1) Ethanol productivity /g?(L·h) −1 Ethanol yield /%
Control (glucose) 100.0 0.406 47.289 1.970 93.102
Detoxified and concentrated CSH 100.0 2.175 43.061 1.794 86.311
Tab.5  
Fig.8  
Strain Substrate Mode Sugar /(g?L −1) Ethanol yield /% Ref.
S. cerevisiae VTTB-08014 Spruce Batch 43.3 74.3 9
Birch 41.8 64.7
S. cerevisiae Corn stover SHFa) NMb) 67.4 35
S. cerevisiae Palm fronds SHF NM 83.5 36
Eucalyptus chips 71.4
S. cerevisiae CICC 1308 Corn stover Batch 100 86.3 This study
Tab.6  
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