Dual enzyme activated fluorescein based fluorescent probe
Maria L. Odyniec1, Jordan E. Gardiner1, Adam C. Sedgwick1,2, Xiao-Peng He3, Steven D. Bull1(), Tony D. James1()
1. Department of Chemistry, University of Bath, Bath, BA2 7AY, UK 2. Department of Chemistry, University of Texas at Austin, Austin, TX 78712-1224, USA 3. Key Laboratory for Advanced Materials & Feringa Nobel Prize Scientist Joint Research Center, East China University of Science and Technology, Shanghai 200237, China
A simple dual analyte fluorescein-based probe (PF3-Glc) was synthesised containing β-glucosidase (β-glc) and hydrogen peroxide (H2O2) trigger units. The presence of β-glc, resulted in fragmentation of the parent molecule releasing glucose and the slightly fluorescent mono-boronate fluorescein (PF3). Subsequently, in the presence of glucose oxidase (GOx), the released glucose was catalytically converted to D-glucono-δ-lactone, which produced H2O2 as a by-product. The GOx-produced H2O2, resulted in classic H2O2-mediated boronate oxidation and the release of the highly emissive fluorophore, fluorescein. This unique cascade reaction lead to an 80-fold increase in fluorescence intensity.
Corresponding Author(s):
Steven D. Bull,Tony D. James
引用本文:
. [J]. Frontiers of Chemical Science and Engineering, 2020, 14(1): 117-121.
Maria L. Odyniec, Jordan E. Gardiner, Adam C. Sedgwick, Xiao-Peng He, Steven D. Bull, Tony D. James. Dual enzyme activated fluorescein based fluorescent probe. Front. Chem. Sci. Eng., 2020, 14(1): 117-121.
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