1. College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China 2. Beijing National Laboratory for Molecular Sciences, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China
Graphene oxide (GO) has been increasingly utilized in the fields of food, biomedicine, environment and other fields because of its benign biocompatible. We encapsulated two kinds of GO with different sizes on yeast cells with the assistance of polyelectrolytes poly (styrene sulfonic acid) sodium salt (PSS) and polyglutamic acid (PGA) (termed as Y@GO). The result does not show a significant difference between the properties of the two types of Y@GO (namely Y@GO1 and Y@GO2). The encapsulation layers are optimized as Yeast/PGA/PSS/PGA/GO/PGA/PSS based on the morphology, dispersity, colony-forming unit, and zeta potential. The encapsulation of GO increases the roughness of the yeast. It is proved that the Y@GO increases the survival time and enhance the activity of yeast cells. The GO shell improves the resistance of yeast cells against pH and salt stresses and extends the storage time of yeast cells.
J H Park, K Kim, J Lee, J Y Choi, D Hong, S H Yang, F Caruso, Y Lee, I S Choi. A cytoprotective and degradable metal-polyphenol nanoshell for single-cell encapsulation. Angewandte Chemie, 2014, 126(46): 12628–12633 https://doi.org/10.1002/ange.201405905
2
J K Lin, X Y Wang, R K Tang. Regulations of organism by materials: a new understanding of biological inorganic chemistry. Journal of Biological Inorganic Chemistry, 2019, 24(4): 467–481 https://doi.org/10.1007/s00775-019-01673-2
3
T S Sreeprasad, P Nguyen, A Alshogeathri, L Hibbeler, F Martinez, N McNeil, V Berry. Graphene quantum dots interfaced with single bacterial spore for bio-electromechanical devices: a graphene cytobot. Scientific Reports, 2015, 5(1): 9138 https://doi.org/10.1038/srep09138
4
H Lee, D Hong, J Y Choi, J Y Kim, S H Lee, H M Kim, S H Yang, I S Choi. Layer-by-layer-based silica encapsulation of individual yeast with thickness control. Chemistry, an Asian Journal, 2015, 10(1): 129–132 https://doi.org/10.1002/asia.201402993
5
B Wang, P Liu, W Jiang, H Pan, X Xu, R Tang. Yeast cells with an artificial mineral shell: protection and modification of living cells by biomimetic mineralization. Angewandte Chemie, 2008, 120(19): 3616–3620 https://doi.org/10.1002/ange.200704718
6
Q X Wu, Y X Guan, S J Yao. Sodium cellulose sulfate: a promising biomaterial used for microcarriers’ designing. Frontiers of Chemical Science and Engineering, 2019, 13(1): 46–58 https://doi.org/10.1007/s11705-018-1723-x
7
I Benucci, M Cerreti, D Maresca, G Mauriello, M Esti. Yeast cells in double layer calcium alginate-chitosan microcapsules for sparkling wine production. Food Chemistry, 2019, 300: 1–10 https://doi.org/10.1016/j.foodchem.2019.125174
8
P K Soma, P D Williams, Y M Lo. Advancements in non-starch polysaccharides researchfor frozen foods and microencapsulation of probiotics. Frontiers of Chemical Engineering in China, 2009, 3(4): 413–426 https://doi.org/10.1007/s11705-009-0254-x
9
M R Dzamukova, E A Naumenko, N I Lannik, R F Fakhrullin. Surface-modified magnetic human cells for scaffold-free tissue engineering. Biomaterials Science, 2013, 1(8): 810–813 https://doi.org/10.1039/c3bm60054h
10
J H Park, D Hong, J Lee, I S Choi. Cell-in-shell hybrids: chemical nanoencapsulation of individual cells. Accounts of Chemical Research, 2016, 49(5): 792–800 https://doi.org/10.1021/acs.accounts.6b00087
11
C C Chen, H J Lin, W J Lu, J J Wu, C H Chew, C H Wong, C Y Yang, H T V Lin. Enhanced repeated-batch bioethanol fermentation of red seaweeds hydrolysates using microtube array membrane-encapsulated yeast. Journal of Biobased Materials and Bioenergy, 2020, 14(1): 138–145 https://doi.org/10.1166/jbmb.2020.1932
12
I Drachuk, O Shchepelina, S Harbaugh, N Kelley-Loughnane, M Stone, V V Tsukruk. Cell surface engineering with edible protein nanoshells. Small, 2013, 9(18): 3128–3137 https://doi.org/10.1002/smll.201202992
13
S A Konnova, I R Sharipova, T A Demina, Y N Osin, D R Yarullina, O N Ilinskaya, Y M Lvov, R F Fakhrullin. Biomimetic cell-mediated three-dimensional assembly of halloysite nanotubes. Chemical Communications, 2013, 49(39): 4208–4210 https://doi.org/10.1039/c2cc38254g
14
J Lee, S H Yang, S P Hong, D Hong, H Lee, H Y Lee, Y G Kim, I S Choi. Chemical control of yeast cell division by cross-linked shells of catechol-grafted polyelectrolyte multilayers. Macromolecular Rapid Communications, 2013, 34(17): 1351–1356 https://doi.org/10.1002/marc.201300444
15
B J Kim, T Park, S Y Park, S W Han, H S Lee, Y G Kim, I S Choi. Control of microbial growth in alginate/polydopamine core/shell microbeads. Chemistry, an Asian Journal, 2015, 10(10): 2130–2133 https://doi.org/10.1002/asia.201500360
16
E I Rabea, E T Badawy, C V Stevens, G Smagghe, W Steurbaut. Chitosan as antimicrobial agent: applications and mode of action. Biomacromolecules, 2003, 4(6): 1457–1465 https://doi.org/10.1021/bm034130m
17
I Drachuk, M K Gupta, V V Tsukruk. Biomimetic coatings to control cellular function through cell surface engineering. Advanced Functional Materials, 2013, 23(36): 4437–4453 https://doi.org/10.1002/adfm.201300038
18
S A Konnova, Y M Lvov, R F Fakhrullin. Magnetic halloysite nanotubes for yeast cell surface engineering. Clay Minerals, 2018, 51(3): 429–433 https://doi.org/10.1180/claymin.2016.051.3.07
19
S K Kiran, S Shukla, A Struck, S Saxena. Surface engineering of graphene oxide shells using lamellar LDH nanostructures. ACS Applied Materials & Interfaces, 2019, 11(22): 20232–20240 https://doi.org/10.1021/acsami.8b21265
20
Y Dong, Y Chang, H Gao, V A León Anchustegui, Q Yu, H Wang, J H Liu, S Wang. Characteristic synergistic cytotoxic effects toward cells in graphene oxide dressing with cadmium and copper ions. Toxicology Research, 2019, 8(6): 908–917 https://doi.org/10.1039/c9tx00146h
21
H Gao, J H Liu, V A L Anchustegui, Y Chang, J Zhang, Y Dong. The protective effects of graphene oxide against the stress from organic solvent by covering Hela cells. Current Nanoscience, 2019, 15(4): 412–419 https://doi.org/10.2174/1573413714666180821112731
22
R F Fakhrullin, A I Zamaleeva, M V Morozov, D I Tazetdinova, F K Alimova, A K Hilmutdinov, R I Zhdanov, M Kahraman, M Culha. Living fungi cells encapsulated in polyelectrolyte shells doped with metal nanoparticles. Langmuir, 2009, 25(8): 4628–4634 https://doi.org/10.1021/la803871z
23
C Doonan, R Ricco, K Liang, D Bradshaw, P Falcaro. Metal-organic frameworks at the biointerface: synthetic strategies and applications. Accounts of Chemical Research, 2017, 50(6): 1423–1432 https://doi.org/10.1021/acs.accounts.7b00090
24
A Rezaei, M Fathi, S M Jafari. Nanoencapsulation of hydrophobic and low-soluble food bioactive compounds within different nanocarriers. Food Hydrocolloids, 2019, 88: 146–162 https://doi.org/10.1016/j.foodhyd.2018.10.003
25
O Schlesinger, L Alfonta. Encapsulation of microorganisms, enzymes, and redox mediators in graphene oxide and reduced graphene oxide. Methods in Enzymology, 2018, 609: 197–219 https://doi.org/10.1016/bs.mie.2018.05.008
M H Wahid, E Eroglu, S M LaVars, K Newton, C T Gibson, U H Stroeher, X J Chen, R A Boulos, C L Raston, S L Harmer. Microencapsulation of bacterial strains in graphene oxide nano-sheets using vortex fluidics. RSC Advances, 2015, 5(47): 37424–37430 https://doi.org/10.1039/C5RA04415D
