A novel flavonol-based colorimetric and turn-on fluorescent probe for rapid determination of hydrazine in real water samples and its bioimaging in vivo andin vitro
Ahui Qin1, Yan Zhang1, Shuai Gong1, Mingxin Li1, Yu Gao1, Xu Xu1, Jie Song2, Zhonglong Wang1(), Shifa Wang1()
1. Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, International Innovation Center for Forest Chemicals and Materials, College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, China 2. Department of Natural Sciences, University of Michigan-Flint, Flint, MI 48502, USA
Hydrazine is extremely toxic and causes severe harm to human body. Herein, a novel fluorescent probe 4-oxo-2-styryl-4H-chromen-3-yl thiophene-2-carboxylate (FHT) was synthesized for detecting hydrazine by using natural cinnamaldehyde as starting material. This probe exhibited significantly enhanced fluorescence response towards hydrazine over various common metal ions, anions, and amine compounds. The detection limit of probe FHT for hydrazine was as low as 0.14 μmol·L–1, significantly lower than that of the threshold value of 0.312 μmol·L–1, imposed by the Environmental Protection Agency. Moreover, the proposed probe was able to detect hydrazine within wide pH (5–10) and linear detection ranges (0–110 μmol·L–1). This probe was employed for determining trace hydrazine in different environmental water samples. The probe FHT-loaded filter paper strips were able to conveniently detect hydrazine of low concentration through distinct naked-eye and fluorescent color changes. Importantly, the probe FHT with low cytotoxicity was successfully applied to visualize hydrazine in living Hela cells and zebrafish.
Corresponding Author(s):
Zhonglong Wang,Shifa Wang
作者简介:
Qingyong Zheng and Ya Gao contributed equally to this work.
引用本文:
. [J]. Frontiers of Chemical Science and Engineering, 2023, 17(1): 24-33.
Ahui Qin, Yan Zhang, Shuai Gong, Mingxin Li, Yu Gao, Xu Xu, Jie Song, Zhonglong Wang, Shifa Wang. A novel flavonol-based colorimetric and turn-on fluorescent probe for rapid determination of hydrazine in real water samples and its bioimaging in vivo andin vitro. Front. Chem. Sci. Eng., 2023, 17(1): 24-33.
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