Optimization of denitrifying phosphorus removal in a pre-denitrification anaerobic/anoxic/post-aeration+ nitrification sequence batch reactor (pre-A2NSBR) system: Nitrate recycling, carbon/nitrogen ratio and carbon source type
1. National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology, Engineering Research Center of Beijing, Beijing University of Technology, Beijing 100124, China 2. School of Marine Science and Technology, Sino-Europe Membrane Technology Research Institute, Harbin Institute of Technology, Weihai 264209, China
A novel two sludge pre-A2NSBR system was developed.
Advanced N and P removal was optimized to treat real domestic wastewater.
Nitrifiers and PAOs were enriched with 19.41% and 26.48%, respectively.
Acetate was demonstrated as the high-quality carbon source type.
Because the efficiency of biological nutrient removal is always limited by the deficient carbon source for the low carbon/nitrogen (C/N) ratio in real domestic sewage, the denitrifying phosphorus removal (DNPR) was developed as a simple and efficient method to remove nitrogen and phosphorous. In addition, this method has the advantage of saving aeration energy while reducing the sludge production. In this context, a pre-denitrification anaerobic/anoxic/post-aeration+ nitrification sequence batch reactor (pre-A2NSBR) system, which could also reduce high ammonia effluent concentration in the traditional two-sludge DNPR process, is proposed in this work. The pre-A2NSBR process was mainly composed of a DNPR SBR and a nitrifying SBR, operating as alternating anaerobic/anoxic/post-aeration+ nitrification sequence. Herein, the long-term performance of different nitrate recycling ratios (0–300%) and C/N ratios (2.5–8.8), carbon source type, and functional microbial community were studied. The results showed that the removal efficiency of total inorganic nitrogen (TIN, including NH4+-N, NO2− -N, and NO3− -N) gradually increased with the nitrate recycling ratios, and the system reached the highest DNPR efficiency of 94.45% at the nitrate recycling ratio of 300%. The optimum C/N ratio was around 3.9–7.3 with a nitrogen and phosphorus removal efficiency of 80.15% and 93.57%, respectively. The acetate was proved to be a high-quality carbon source for DNPR process. The results of fluorescence in situ hybridization (FISH) analysis indicated that nitrifiers and phosphorus accumulating organisms (PAOs) were accumulated with a proportion of 19.41% and 26.48%, respectively.
Ahn J, Daidou T, Tsuneda S, Hirata A ( 2002 ). Characterization of denitrifying phosphate-accumulating organisms cultivated under different electron acceptor conditions using polymerase chain reaction-denaturing gradient gel electrophoresis assay. Water Research, 36(2): 403–412 https://doi.org/10.1016/S0043-1354(01)00222-6
pmid: 11827346
2
Boiran B, Couton Y, Germon J C (1996). Nitrification and denitrification of liquid lagoon piggery waste in a biofilm infiltration-percolation aerated system (BIPAS) reactor. Bioresource Technology, 55(1): 63–77 https://doi.org/10.1016/0960-8524(95)00142-5
3
Bortone G, Saltarelli R, Alonso V, Sorm R, Wanner J, Tilche A ( 1996 ). Biological anoxic phosphorus removal—The dephanox process. Water Science and Technology, 34(1–2): 119–128 https://doi.org/10.2166/wst.1996.0363
4
Carucci A, Ramadori R, Rossetti S, Tomei M C ( 1996 ). Kinetics of denitrification reactions in single sludge systems. Water Research, 30(1): 51–56 https://doi.org/10.1016/0043-1354(95)00127-7
5
Chen Y, Peng C, Wang J, Ye L, Zhang L, Peng Y ( 2011 ). Effect of nitrate recycling ratio on simultaneous biological nutrient removal in a novel anaerobic/anoxic/oxic (A2/O)-biological aerated filter (BAF) system. Bioresource Technology, 102(10): 5722–5727 https://doi.org/10.1016/j.biortech.2011.02.114
pmid: 21459571
6
Chen Y, Randall A A, McCue T ( 2004 ). The efficiency of enhanced biological phosphorus removal from real wastewater affected by different ratios of acetic to propionic acid. Water Research, 38(1): 27–36 https://doi.org/10.1016/j.watres.2003.08.025
pmid: 14630100
7
Chen Y Z, Li B K, Ye L, Peng Y Z ( 2015 ). The combined effects of COD/N ratio and nitrate recycling ratio on nitrogen and phosphorus removal in anaerobic/anoxic/aerobic (A2/O)-biological aerated filter (BAF) systems. Biochemical Engineering Journal, 93(10): 235–242 https://doi.org/10.1016/j.bej.2014.10.005
8
Crocetti G R, Hugenholtz P, Bond P L, Schuler A, Keller J, Jenkins D, Blackall L L ( 2000 ). Identification of polyphosphate-accumulating organisms and design of 16S rRNA-directed probes for their detection and quantitation. Applied and Environmental Microbiology, 66(3): 1175–1182 https://doi.org/10.1128/AEM.66.3.1175-1182.2000
pmid: 10698788
9
Du D, Zhang C, Zhao K, Sun G, Zou S, Yuan L, He S ( 2018 ). Effect of different carbon sources on performance of an A2N-MBR process and its microbial community structure. Frontiers of Environmental Science & Engineering, 12(2): 4 https://doi.org/10.1007/s11783-017-0981-z
10
Elefsiniotis P, Li D ( 2006 ). The effect of temperature and carbon source on denitrification using volatile fatty acids. Biochemical Engineering Journal, 28(2): 148–155 https://doi.org/10.1016/j.bej.2005.10.004
11
Ge S, Zhu Y, Lu C, Wang S, Peng Y ( 2012 ). Full-scale demonstration of step feed concept for improving an anaerobic/anoxic/aerobic nutrient removal process. Bioresource Technology, 120(3): 305–313 https://doi.org/10.1016/j.biortech.2012.06.030
pmid: 22796144
12
Gong Y K, Peng Y Z, Wang S Y, Wang S ( 2014 ). Production of N2O in two biologic nitrogen removal processes: A comparison between conventional and short-cut nitrogen removal processes. Frontiers of Environmental Science & Engineering, 8(4): 589–597 https://doi.org/10.1007/s11783-013-0571-7
13
Kuba T, VanLoosdrecht M C M, Heijnen J J ( 1996 ). Phosphorus and nitrogen removal with minimal cod requirement by integration of denitrifying dephosphatation and nitrification in a two-sludge system. Water Research, 30(7): 1702–1710 https://doi.org/10.1016/0043-1354(96)00050-4
14
Lee H S, Park S J, Yoon T I ( 2002 ). Wastewater treatment in a hybrid biological reactor using powdered minerals: Effects of organic loading rates on COD removal and nitrification. Process Biochemistry, 38(1): 81–88 https://doi.org/10.1016/S0032-9592(02)00044-4
15
Lee N M, Welander T ( 1996 ). The effect of different carbon sources on respiratory denitrification in biological wastewater treatment. Journal of Fermentation and Bioengineering, 82(3): 277–285 https://doi.org/10.1016/0922-338X(96)88820-9
16
Liu X, Wang H, Yang Q, Li J, Zhang Y, Peng Y ( 2017 ). Online control of biofilm and reducing carbon dosage in denitrifying biofilter: Pilot and full-scale application. Frontiers of Environmental Science & Engineering, 11(1): 4 https://doi.org/10.1007/s11783-017-0895-9
17
Ma B, Wang S Y, Zhu G B, Ge S J, Wang J M, Ren N Q, Peng Y Z ( 2013 ). Denitrification and phosphorus uptake by DPAOs using nitrite as an electron acceptor by step-feed strategies. Frontiers of Environmental Science & Engineering, 7(2): 267–272 https://doi.org/10.1007/s11783-012-0439-2
18
Ma Y, Peng Y Z, Wang X L ( 2009 ). Improving nutrient removal of the AAO process by an influent bypass flow by denitrifying phosphorus removal. Desalination, 246(1–3): 534–544 https://doi.org/10.1016/j.desal.2008.04.061
19
Paungfoo C, Prasertsan P, Burrell P C, Intrasungkha N, Blackall L L ( 2007 ). Nitrifying bacterial communities in an aquaculture wastewater treatment system using fluorescence in situ hybridization (FISH), 16S rRNA gene cloning, and phylogenetic analysis. Biotechnology and Bioengineering, 97(4): 985–990 https://doi.org/10.1002/bit.21270
pmid: 17115448
20
Pijuan M, Casas C, Baeza J A ( 2009 ). Polyhydroxyalkanoate synthesis using different carbon sources by two enhanced biological phosphorus removal microbial communities. Process Biochemistry, 44(1): 97–105 https://doi.org/10.1016/j.procbio.2008.09.017
21
Sun S P, Nàcher C P i, Merkey B, Zhou Q, Xia S Q, Yang D H, Sun J H, Smets B F (2010). Effective biological nitrogen removal treatment processes for domestic wastewaters with low C/N ratios: A review. Environmental Engineering Science, 27(2): 111–126
22
Thomas M, Wright P, Blackall L, Urbain V, Keller J ( 2003 ). Optimisation of Noosa BNR plant to improve performance and reduce operating costs. Water Science and Technology, 47(12): 141–148 https://doi.org/10.2166/wst.2003.0639
pmid: 12926681
23
van Loosdrecht M C M, Brandse F A, de Vries A C ( 1998 ). Upgrading of waste water treatment processes for integrated nutrient removal- The BCFS (R) process. Water Science and Technology, 37(9): 209–217 https://doi.org/10.2166/wst.1998.0359
24
Walters E, Hille A, He M, Ochmann C, Horn H ( 2009 ). Simultaneous nitrification/denitrification in a biofilm airlift suspension (BAS) reactor with biodegradable carrier material. Water Research, 43(18): 4461–4468 https://doi.org/10.1016/j.watres.2009.07.005
pmid: 19640560
25
Wang X, Wang S, Xue T, Li B, Dai X, Peng Y ( 2015 ). Treating low carbon/nitrogen (C/N) wastewater in simultaneous nitrification-endogenous denitrification and phosphorous removal (SNDPR) systems by strengthening anaerobic intracellular carbon storage. Water Research, 77: 191–200 https://doi.org/10.1016/j.watres.2015.03.019
pmid: 25875928
26
Wang Y, Geng J, Ren Z, Guo G, Wang C, Wang H ( 2013 ). Effect of COD/N and COD/P ratios on the PHA transformation and dynamics of microbial community structure in a denitrifying phosphorus removal process. Journal of Chemical Technology and Biotechnology (Oxford, Oxfordshire), 88(7): 1228–1236 https://doi.org/10.1002/jctb.3962
27
Wanner J, Cech J S, Kos M ( 1992 ). New process design for biological nutrient removal. Water Science and Technology, 25(4–5): 445–448 https://doi.org/10.2166/wst.1992.0532
28
Yagci N, Cokgor E U, Artan N, Randall C, Orhon D ( 2007 ). The effect of substrate on the composition of polyhydroxyalkanoates in enhanced biological phosphorus removal. Journal of Chemical Technology and Biotechnology (Oxford, Oxfordshire), 82(3): 295–303 https://doi.org/10.1002/jctb.1672
29
Yang Y, Zhang L, Shao H, Zhang S, Gu P, Peng Y ( 2017 ). Enhanced nutrients removal from municipal wastewater through biological phosphorus removal followed by partial nitritation/anammox. Frontiers of Environmental Science & Engineering, 11(2): 8 https://doi.org/10.1007/s11783-017-0911-0
30
Yu J, Si Y ( 2004 ). Metabolic carbon fluxes and biosynthesis of polyhydroxyalkanoates in Ralstonia eutropha on short chain fatty acids. Biotechnology Progress, 20(4): 1015–1024 https://doi.org/10.1021/bp034380e
pmid: 15296425
31
Zeng R J, Lemaire R, Yuan Z, Keller J ( 2003 ). Simultaneous nitrification, denitrification, and phosphorus removal in a lab-scale sequencing batch reactor. Biotechnology and Bioengineering, 84(2): 170–178 https://doi.org/10.1002/bit.10744
pmid: 12966573
32
Zeng W, Li B, Yang Y, Wang X, Li L, Peng Y ( 2014 ). Impact of nitrite on aerobic phosphorus uptake by poly-phosphate accumulating organisms in enhanced biological phosphorus removal sludges. Bioprocess and Biosystems Engineering, 37(2): 277–287 https://doi.org/10.1007/s00449-013-0993-4
pmid: 23771179
33
Zeng W, Peng Y Z, Wang S Y (2004). A two-stage SBR process for removal of organic substrate and nitrogen via nitrite-type nitrification-denitrification. Journal of Environmental Science and Health Part A—Toxic/Hazardous Substances & Environmental Engineering, 39(8): 2229–2239
34
Zhang M, Peng Y, Wang C, Wang C, Zhao W, Zeng W ( 2016a ). Optimization denitrifying phosphorus removal at different hydraulic retention times in a novel anaerobic anoxic oxic-biological contact oxidation process. Biochemical Engineering Journal, 106: 26–36 https://doi.org/10.1016/j.bej.2015.10.027
35
Zhang M, Wang C, Peng Y, Wang S, Jia F, Zeng W ( 2016b ). Organic substrate transformation and sludge characteristics in the integrated anaerobic anoxic oxic–biological contact oxidation (A2/O–BCO) system treating wastewater with low carbon/nitrogen ratio. Chemical Engineering Journal, 283: 47–57 https://doi.org/10.1016/j.cej.2015.04.111
36
Zhang M, Yang Q, Zhang J, Wang C, Wang S, Peng Y ( 2016c ). Enhancement of denitrifying phosphorus removal and microbial community of long-term operation in an anaerobic anoxic oxic-biological contact oxidation system. Journal of Bioscience and Bioengineering, 122(4): 456–466 https://doi.org/10.1016/j.jbiosc.2016.03.019
pmid: 27133708
37
Zhang W, Hou F, Peng Y Z, Liu Q S, Wang S Y ( 2014 ). Optimizing aeration rate in an external nitrification-denitrifying phosphorus removal (ENDPR) system for domestic wastewater treatment. Chemical Engineering Journal, 245: 342–347 https://doi.org/10.1016/j.cej.2014.01.045
38
Zhang W, Peng Y, Ren N, Liu Q, Chen Y ( 2013 ). Improvement of nutrient removal by optimizing the volume ratio of anoxic to aerobic zone in AAO-BAF system. Chemosphere, 93(11): 2859–2863 https://doi.org/10.1016/j.chemosphere.2013.08.047
pmid: 24035691
39
Zhao W, Huang Y, Wang M, Pan C, Li X, Peng Y, Li B ( 2018 ). Post-endogenous denitrification and phosphorus removal in an alternating anaerobic/oxic/anoxic (AOA) system treating low carbon/nitrogen (C/N) domestic wastewater. Chemical Engineering Journal, 339: 450–458 https://doi.org/10.1016/j.cej.2018.01.096
40
Zhao W, Zhang Y, Lv D, Wang M, Peng Y, Li B ( 2016 ). Advanced nitrogen and phosphorus removal in the pre-denitrification anaerobic/anoxic/aerobic nitrification sequence batch reactor (pre-A2NSBR) treating low carbon/nitrogen (C/N) wastewater. Chemical Engineering Journal, 302: 296–304 https://doi.org/10.1016/j.cej.2016.05.012
