1. College of Environmental Science and Engineering, Taiyuan University of Technology; Shanxi Key Laboratory of Earth Surface Processes and Resource Ecological Security in Fenhe River Basin; Shanxi Engineering Research Center of Low Carbon Remediation for Water and Soil Pollution in Yellow River Basin, Jinzhong 030600, China 2. Center for Environmental Remediation, Institute of Geographic Sciences and Natural Resources Research, Chinese Academy of Sciences, Beijing 100101, China 3. University of Chinese Academy of Sciences, Beijing 100049, China 4. Shanxi Transportation Holding Ecological Environment Co., Ltd., Shanxi 030000, China 5. Beijing Key Lab for Source Control Technology of Water Pollution, College of Environmental Science and Engineering, Beijing Forestry University, Beijing 100083, China
● WSC improves physicochemical properties of soil for plant growth.
● Water-soluble and acid-extractable Pb in soil increase with WSC dose.
● Amino and hydroxyl groups in WSC play important roles in mobilizing Pb in soil.
● WSC improves phytoremediation capacity of Pb-contaminated soil by H. spectabile .
Water-soluble chitosan (WSC) has been studied for its ability to mobilize soil Pb and promote the phytoremediation by Hylotelephium spectabile in Pb-contaminated fields. We aimed to clarify the internal mechanism by which WSC impacts phytoremediation by examining plant growth and Pb accumulation performance of H. spectabile as well as the Pb form, functional groups, and mineral phases of Pb-contaminated soil. WSC effectively decreased soil pH and activated Pb migration in rhizosphere soils, with a considerable increase in water-soluble and acid-extractable Pb by 29%–102% and 9%–65%, respectively, and a clear decreasing trend in reducible and oxidizable Pb. Fourier-transform infrared spectroscopy revealed a significant increase in amino and hydroxyl groups in the soil generated by WSC. The coordination of Pb with amino and hydroxyl groups may play an important role in the formation of Pb complexes and activation of Pb in soil. In field trials, the application of WSC significantly increased Pb accumulation in H. spectabile by 125.44%, reaching 92 g/hm2. Moreover, the organic matter and nitrogen in the soils were increased by WSC, which improved the growth conditions of H. spectabile. No obvious growth inhibition was observed in either the pot or field trials. Therefore, WSC is a promising chelating agent for mobilizing Pb in soil. Additionally, WSC can be potentially used to boost H. spectabil-mediated phytoremediation of Pb-contaminated farmland.
M Ahmad , K Manzoor , S Ikram . (2017). Versatile nature of hetero-chitosan based derivatives as biodegradable adsorbent for heavy metal ions: a review. International Journal of Biological Macromolecules, 105: 190–203 https://doi.org/10.1016/j.ijbiomac.2017.07.008
2
P O Boamah , Y Huang , M Hua , Q Zhang , Y Liu , J Onumah , W Wang , Y Song . (2015). Removal of cadmium from aqueous solution using low molecular weight chitosan derivative. Carbohydrate Polymers, 122: 255–264 https://doi.org/10.1016/j.carbpol.2015.01.004
3
O K Borggaard , P E Holm , B W Strobel . (2019). Potential of dissolved organic matter (DOM) to extract As, Cd, Co, Cr, Cu, Ni, Pb and Zn from polluted soils: a review. Geoderma, 343: 235–246 https://doi.org/10.1016/j.geoderma.2019.02.041
4
Y Cao , S Zhang , Q Zhong , G Wang , X Xu , T Li , L Wang , Y Jia , Y Li . (2018). Feasibility of nanoscale zero-valent iron to enhance the removal efficiencies of heavy metals from polluted soils by organic acids. Ecotoxicology and Environmental Safety, 162: 464–473 https://doi.org/10.1016/j.ecoenv.2018.07.036
5
A Chen , C Shang , J Shao , Y Lin , S Luo , J Zhang , H Huang , M Lei , Q Zeng . (2017). Carbon disulfide-modified magnetic ion-imprinted chitosan-Fe(III): a novel adsorbent for simultaneous removal of tetracycline and cadmium. Carbohydrate Polymers, 155: 19–27 https://doi.org/10.1016/j.carbpol.2016.08.038
6
L Chen , J Y Yang , D Wang . (2020). Phytoremediation of uranium and cadmium contaminated soils by sunflower (Helianthus annuus L.) enhanced with biodegradable chelating agents. Journal of Cleaner Production, 263: 121491 https://doi.org/10.1016/j.jclepro.2020.121491
7
C Ganglo , J Rui , Q Zhu , J Shan , Z Wang , F Su , D Liu , J Xu , M Guo , J Qian . (2020). Chromium (III) coordination capacity of chitosan. International Journal of Biological Macromolecules, 148: 785–792 https://doi.org/10.1016/j.ijbiomac.2020.01.203
8
S Goswami , S Das . (2015). A Study on cadmium phytoremediation potential of indian mustard, Brassica juncea. International Journal of Phytoremediation, 17(6): 583–588 https://doi.org/10.1080/15226514.2014.935289
9
J Guo , R Feng , Y Ding , R Wang . (2014). Applying carbon dioxide, plant growth-promoting rhizobacterium and EDTA can enhance the phytoremediation efficiency of ryegrass in a soil polluted with zinc, arsenic, cadmium and lead. Journal of Environmental Management, 141: 1–8 https://doi.org/10.1016/j.jenvman.2013.12.039
10
J Guo , Y Wei , J Yang , T Chen , G Zheng , T Qian , X Liu , X Meng , M He . (2023). Cultivars and oil extraction techniques affect Cd/Pb contents and health risks in oil of rapeseed grown on Cd/Pb-contaminated farmland. Frontiers of Environmental Science and Engineering, 17(7): 87 https://doi.org/10.1007/s11783-023-1687-z
11
J Guo , J Yang , J Yang , G Zheng , T Chen , J Huang , J Bian , X Meng . (2020). Water-soluble chitosan enhances phytoremediation efficiency of cadmium by Hylotelephium spectabile in contaminated soils. Carbohydrate Polymers, 246: 116559 https://doi.org/10.1016/j.carbpol.2020.116559
12
I Hamed , F Özogul , J M Regenstein . (2016). Industrial applications of crustacean by-products (chitin, chitosan, and chitooligosaccharides): a review. Trends in Food Science and Technology, 48: 40–50 https://doi.org/10.1016/j.tifs.2015.11.007
13
Y Han , L Zhang , J Gu , J Zhao , J Fu . (2018). Citric acid and EDTA on the growth, photosynthetic properties and heavy metal accumulation of Iris halophila Pall. cultivated in Pb mine tailings. International Biodeterioration and Biodegradation, 128: 15–21 https://doi.org/10.1016/j.ibiod.2016.05.011
14
C J Harbort , M Hashimoto , H Inoue , Y Niu , R Guan , A D Rombolà , S Kopriva , M J E E Voges , E S Sattely , R Garrido-Oter . et al.. (2020). Root-secreted coumarins and the microbiota interact to improve iron nutrition in Arabidopsis. Cell Host & Microbe, 28(6): 825–837.e6 https://doi.org/10.1016/j.chom.2020.09.006
15
S M Harmon . (2022). Biodegradable chelate-assisted phytoextraction of metals from soils and sediments. Current Opinion in Green and Sustainable Chemistry, 37: 100677 https://doi.org/10.1016/j.cogsc.2022.100677
16
W Hu , Y Niu , H Zhu , K Dong , D Wang , F Liu . (2021). Remediation of zinc-contaminated soils by using the two-step washing with citric acid and water-soluble chitosan. Chemosphere, 282: 131092 https://doi.org/10.1016/j.chemosphere.2021.131092
17
I Hui , E Pasquier , A Solberg , K Agrenius , J Håkansson , G Chinga-Carrasco . (2023). Biocomposites containing poly(lactic acid) and chitosan for 3D printing: assessment of mechanical, antibacterial and in vitro biodegradability properties. Journal of the Mechanical Behavior of Biomedical Materials, 147: 106136 https://doi.org/10.1016/j.jmbbm.2023.106136
18
H S Jung , M H Kim , J Y Shin , S R Park , J Y Jung , W H Park . (2018). Electrospinning and wound healing activity of β-chitin extracted from cuttlefish bone. Carbohydrate Polymers, 193: 205–211 https://doi.org/10.1016/j.carbpol.2018.03.100
19
A Khan , K A Alamry . (2021). Recent advances of emerging green chitosan-based biomaterials with potential biomedical applications: a review. Carbohydrate Research, 506: 108368 https://doi.org/10.1016/j.carres.2021.108368
20
M N V R Kumar , R A A Muzzarelli , C Muzzarelli , H Sashiwa , A J Domb . (2004). Chitosan chemistry and pharmaceutical perspectives. Chemical Reviews, 104(12): 6017–6084 https://doi.org/10.1021/cr030441b
21
J Lee , K Sung . (2014). Effects of chelates on soil microbial properties, plant growth and heavy metal accumulation in plants. Ecological Engineering, 73: 386–394 https://doi.org/10.1016/j.ecoleng.2014.09.053
22
J Li , M Chen , X Yang , L Zhang . (2024). Effect and mechanisms of soil functional groups in bacterial-enhanced cadmium contaminated soil phytoremediation. Environmental Technology and Innovation, 33: 103531 https://doi.org/10.1016/j.eti.2024.103531
23
Y Li , R Xu , C Ma , J Yu , S Lei , Q Han , H Wang . (2023). Potential functions of engineered nanomaterials in cadmium remediation in soil-plant system: a review. Environmental Pollution, 336: 122340 https://doi.org/10.1016/j.envpol.2023.122340
24
K Liu , X Guan , C Li , K Zhao , X Yang , R Fu , Y Li , F Yu . (2022). Global perspectives and future research directions for the phytoremediation of heavy metal-contaminated soil: a knowledge mapping analysis from 2001 to 2020. Frontiers of Environmental Science and Engineering, 16(6): 73 https://doi.org/10.1007/s11783-021-1507-2
25
C S Lwin , B H Seo , H U Kim , G Owens , K R Kim . (2018). Application of soil amendments to contaminated soils for heavy metal immobilization and improved soil quality: a critical review. Soil Science and Plant Nutrition, 64(2): 156–167 https://doi.org/10.1080/00380768.2018.1440938
26
S Mekahlia , B Bouzid . (2009). Chitosan-Copper (II) complex as antibacterial agent: synthesis, characterization and coordinating bond- activity correlation study. Physics Procedia, 2(3): 1045–1053 https://doi.org/10.1016/j.phpro.2009.11.061
27
of Environmental Protection Ministryof Land MinistryResources (2014). Reports on China’s Soil Contamination Survey. Beijing: Ministry of Environmental Protection and Ministry of Land and Resources (in Chinese)
28
D Moulick , D Ghosh , J Mandal , S Bhowmick , D Mondal , S Choudhury , S C Santra , M Vithanage , J K Biswas . (2023). A cumulative assessment of plant growth stages and selenium supplementation on arsenic and micronutrients accumulation in rice grains. Journal of Cleaner Production, 386: 135764 https://doi.org/10.1016/j.jclepro.2022.135764
29
A Mousavi , L Pourakbar , Moghaddam S Siavash , J Popović-Djordjević . (2021). The effect of the exogenous application of EDTA and maleic acid on tolerance, phenolic compounds, and cadmium phytoremediation by okra (Abelmoschus esculentus L.) exposed to Cd stress. Journal of Environmental Chemical Engineering, 9(4): 105456 https://doi.org/10.1016/j.jece.2021.105456
30
S Mwelwa , D Chungu , F Tailoka , D Beesigamukama , C Tanga . (2023). Biotransfer of heavy metals along the soil-plant-edible insect-human food chain in Africa. Science of the Total Environment, 881: 163150 https://doi.org/10.1016/j.scitotenv.2023.163150
31
U Najeeb , W Ahmad , M H Zia , M Zaffar , W Zhou . (2017). Enhancing the lead phytostabilization in wetland plant Juncus effusus L. through somaclonal manipulation and EDTA enrichment. Arabian Journal of Chemistry, 10: S3310–S3317 https://doi.org/10.1016/j.arabjc.2014.01.009
32
S Nayeri , Z Dehghanian , B Asgari Lajayer , A Thomson , T Astatkie , G W Price . (2023). CRISPR/Cas9-mediated genetically edited ornamental and aromatic plants: a promising technology in phytoremediation of heavy metals. Journal of Cleaner Production, 428: 139512 https://doi.org/10.1016/j.jclepro.2023.139512
33
A Oberlintner , M Bajić , G Kalčíková , B Likozar , U Novak . (2021). Biodegradability study of active chitosan biopolymer films enriched with Quercus polyphenol extract in different soil types. Environmental Technology & Innovation, 21: 101318 https://doi.org/10.1016/j.eti.2020.101318
34
I Orienti , T Cerchiara , B Luppi , F Bigucci , G Zuccari , V Zecchi . (2002). Influence of different chitosan salts on the release of sodium diclofenac in colon-specific delivery. International Journal of Pharmaceutics, 238(1–2): 51–59 https://doi.org/10.1016/S0378-5173(02)00060-1
35
A Padilla-Rodríguez , E E Codling . (2016). Potential of chitosan (chemically modified chitin) for extraction of lead-arsenate contaminated soils. Communications in Soil Science and Plant Analysis, 47(13–14): 1650–1663 https://doi.org/10.1080/00103624.2016.1206123
36
F S Pereira , S Lanfredi , E R P González , Silva Agostini D L Da , H M Gomes , Santos Medeiros R Dos . (2017). Thermal and morphological study of chitosan metal complexes. Journal of Thermal Analysis and Calorimetry, 129(1): 291–301 https://doi.org/10.1007/s10973-017-6146-2
37
R Rathika , P Srinivasan , J Alkahtani , L A Al-Humaid , M S Alwahibi , R Mythili , T Selvankumar . (2021). Influence of biochar and EDTA on enhanced phytoremediation of lead contaminated soil by Brassica juncea. Chemosphere, 271: 129513 https://doi.org/10.1016/j.chemosphere.2020.129513
38
M V Rechberger , F Zehetner , I N Candra , M H Gerzabek . (2020). Impact of soil development on Cu sorption along gradients of soil age and moisture on the Galápagos Islands. Catena, 189: 104507 https://doi.org/10.1016/j.catena.2020.104507
39
L A Schaider , D R Parker , D L Sedlak . (2006). Uptake of EDTA-complexed Pb, Cd and Fe by solution- and sand-cultured Brassica juncea. Plant and Soil, 286(1–2): 377–391 https://doi.org/10.1007/s11104-006-9049-8
40
C Shang , Y Chai , L Peng , J Shao , H Huang , A Chen . (2023). Remediation of Cr(VI) contaminated soil by chitosan stabilized FeS composite and the changes in microorganism community. Chemosphere, 327: 138517 https://doi.org/10.1016/j.chemosphere.2023.138517
41
J Shi , D Zhao , F Ren , L Huang . (2023). Spatiotemporal variation of soil heavy metals in China: the pollution status and risk assessment. Science of the Total Environment, 871: 161768 https://doi.org/10.1016/j.scitotenv.2023.161768
42
J Song , Y Chen , H Mi , R Xu , W Zhang , C Wang , C Rensing , Y Wang . (2024). Prevalence of antibiotic and metal resistance genes in phytoremediated cadmium and zinc contaminated soil assisted by chitosan and Trichoderma harzianum. Environment International, 183: 108394 https://doi.org/10.1016/j.envint.2023.108394
43
S Kumar , S Banerjee , S Ghosh , S Majumder , J Mandal , P K Roy , P Bhattacharyya . (2024). Appraisal of pollution and health risks associated with coal mine contaminated soil using multimodal statistical and Fuzzy-TOPSIS approaches. Frontiers of Environmental Science and Engineering, 18(5): 60 https://doi.org/10.1007/s11783-024-1820-7
44
V Suthar , K S Memon , M Mahmood-Ul-Hassan . (2014). EDTA-enhanced phytoremediation of contaminated calcareous soils: heavy metal bioavailability, extractability, and uptake by maize and sesbania. Environmental Monitoring and Assessment, 186(6): 3957–3968 https://doi.org/10.1007/s10661-014-3671-3
45
U Upadhyay , I Sreedhar , S A Singh , C M Patel , K L Anitha . (2021). Recent advances in heavy metal removal by chitosan based adsorbents. Carbohydrate Polymers, 251: 117000 https://doi.org/10.1016/j.carbpol.2020.117000
46
G WangS ZhangX XuQ ZhongC ZhangY JiaT LiO DengY (2016) Li. Heavy metal removal by GLDA washing: optimization, redistribution, recycling, and changes in soil fertility. Science of the Total Environment, 569–570: 557–568
47
J Wang , M Aghajani Delavar . (2023). Techno-economic analysis of phytoremediation: a strategic rethinking. Science of the Total Environment, 902: 165949 https://doi.org/10.1016/j.scitotenv.2023.165949
48
Y Xia , D Qiu , Z Lyv , J Zhang , N Singh , K Shih , Y Tang . (2023). Controlled sintering for cadmium stabilization by beneficially using the dredged river sediment. Frontiers of Environmental Science and Engineering, 17(5): 61 https://doi.org/10.1007/s11783-023-1661-9
49
J Yang , J Yang , J Huang . (2017). Role of co-planting and chitosan in phytoextraction of As and heavy metals by Pteris vittata and castor bean: a field case. Ecological Engineering, 109: 35–40 https://doi.org/10.1016/j.ecoleng.2017.09.001
50
J Yang , X Xin , X Zhang , X Zhong , W Yang , G Ren , A Zhu . (2023). Effects of soil physical and chemical properties on phosphorus adsorption-desorption in fluvo-aquic soil under conservation tillage. Soil and Tillage Research, 234: 105840 https://doi.org/10.1016/j.still.2023.105840
51
Z Yang , W Lu , Y Long , X Bao , Q Yang . (2011). Assessment of heavy metals contamination in urban topsoil from Changchun City, China. Journal of Geochemical Exploration, 108(1): 27–38 https://doi.org/10.1016/j.gexplo.2010.09.006
52
K Yu , J Ho , E Mccandlish , B Buckley , R Patel , Z Li , N C Shapley . (2013). Copper ion adsorption by chitosan nanoparticles and alginate microparticles for water purification applications. Colloids and Surfaces. A, Physicochemical and Engineering Aspects, 425: 31–41 https://doi.org/10.1016/j.colsurfa.2012.12.043
53
Q Yu , X Hu , J Ma , J Ye , W Sun , Q Wang , H Lin . (2020). Effects of long-term organic material applications on soil carbon and nitrogen fractions in paddy fields. Soil & Tillage Research, 196: 104483 https://doi.org/10.1016/j.still.2019.104483
54
H Zhang , Q Guo , J Yang , J Ma , G Chen , T Chen , G Zhu , J Wang , G Zhang , X Wang , C Shao . (2016). Comparison of chelates for enhancing Ricinus communis L. phytoremediation of Cd and Pb contaminated soil. Ecotoxicology and Environmental Safety, 133: 57–62 https://doi.org/10.1016/j.ecoenv.2016.05.036
