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Frontiers of Medicine

ISSN 2095-0217

ISSN 2095-0225(Online)

CN 11-5983/R

邮发代号 80-967

2019 Impact Factor: 3.421

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Frontiers of Medicine  2019, Vol. 13 Issue (5): 602-609   https://doi.org/10.1007/s11684-019-0704-x
  本期目录
PET imaging on neurofunctional changes after optogenetic stimulation in a rat model of panic disorder
Xiao He1,2,3, Chentao Jin1,2,3, Mindi Ma1,2,3, Rui Zhou1,2,3, Shuang Wu1,2,3, Haoying Huang1,2,3, Yuting Li1,2,3, Qiaozhen Chen2,4, Mingrong Zhang5(), Hong Zhang1,2,3(), Mei Tian1,2,3()
1. Department of Nuclear Medicine and Medical PET Center, The Second Hospital of Zhejiang University School of Medicine, Hangzhou 310009, China
2. Key Laboratory of Medical Molecular Imaging of Zhejiang Province, Hangzhou 310009, China
3. Institute of Nuclear Medicine and Molecular Imaging, Zhejiang University, Hangzhou 310009, China
4. Department of Psychiatry, The Second Hospital of Zhejiang University School of Medicine, Hangzhou 310009, China
5. Department of Advanced Nuclear Medicine Sciences, National Institute of Radiological Sciences,?National Institutes for Quantum and Radiological Science and Technology,?Anagawa 4-9-1, Inage-ku, Chiba, 263-8555, Japan
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Abstract：

Panic disorder (PD) is an acute paroxysmal anxiety disorder with poorly understood pathophysiology. The dorsal periaqueductal gray (dPAG) is involved in the genesis of PD. However, the downstream neurofunctional changes of the dPAG during panic attacks have yet to be evaluated in vivo. In this study, optogenetic stimulation to the dPAG was performed to induce panic-like behaviors, and in vivo positron emission tomography (PET) imaging with 18F-flurodeoxyglucose (18F-FDG) was conducted to evaluate neurofunctional changes before and after the optogenetic stimulation. Compared with the baseline, post-optogenetic stimulation PET imaging demonstrated that the glucose metabolism significantly increased (P<0.001) in dPAG, the cuneiform nucleus, the cerebellar lobule, the cingulate cortex, the alveus of the hippocampus, the primary visual cortex, the septohypothalamic nucleus, and the retrosplenial granular cortex but significantly decreased (P<0.001) in the basal ganglia, the frontal cortex, the forceps minor corpus callosum, the primary somatosensory cortex, the primary motor cortex, the secondary visual cortex, and the dorsal lateral geniculate nucleus. Taken together, these data indicated that in vivo PET imaging can successfully detect downstream neurofunctional changes involved in the panic attacks after optogenetic stimulation to the dPAG.
Key words： panic disorder (PD)    positron emission tomography (PET)    optogenetics    dorsal periaqueductal gray (dPAG)
收稿日期: 2019-04-02      出版日期: 2019-10-14
Corresponding Author(s): Mingrong Zhang,Hong Zhang,Mei Tian   
 引用本文:   
. [J]. Frontiers of Medicine, 2019, 13(5): 602-609.
Xiao He, Chentao Jin, Mindi Ma, Rui Zhou, Shuang Wu, Haoying Huang, Yuting Li, Qiaozhen Chen, Mingrong Zhang, Hong Zhang, Mei Tian. PET imaging on neurofunctional changes after optogenetic stimulation in a rat model of panic disorder. Front. Med., 2019, 13(5): 602-609.
 链接本文:  
https://academic.hep.com.cn/fmd/CN/10.1007/s11684-019-0704-x
https://academic.hep.com.cn/fmd/CN/Y2019/V13/I5/602
Fig.1  
Brain region Coordinate (mm) Ke T value Z score Puncorrected
x y z
Increased
Dorsal periaqueductal gray 1 5 -7 3051 15.66 4.27 <0.001
Cuneiform nucleus -2 6 -8 7.83 3.46 <0.001
Cerebellar lobule 1 2 -13 1312 10.47 3.81 <0.001
Cingulate cortex 1 2 2 221 7.05 3.32 <0.001
Alveus of the hippocampus -6 4 -7 1305 8.44 3.55 <0.001
Primary visual cortex -4 2 -9 7.87 3.46 <0.001
Septohypothalamic nucleus -1 6 0 962 18.40 4.45 <0.001
Retrosplenial granular cortex 0 2 -3 158 7.47 3.40 <0.001
Decreased
Basal ganglia 3 5 2 1694 8.00 3.49 <0.001
Frontal cortex 4 4 4 8.04 3.49 <0.001
Forceps minor corpus callosum -3 3 4 7173 19.35 4.50 <0.001
Primary somatosensory cortex -5 5 1 16.93 4.36 <0.001
Primary motor cortex -3 2 2 10.17 3.78 <0.001
Secondary visual cortex -3 1 -5 890 14.61 4.20 <0.001
Dorsal lateral geniculate nucleus 3 4 -4 1073 11.95 3.97 <0.001
Tab.1  
Fig.2  
Fig.3  
Fig.4  
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