Distinct mononuclear diploid cardiac subpopulation with minimal cell–cell communications persists in embryonic and adult mammalian heart

doi:10.1007/s11684-023-0987-9

Frontiers of Medicine

2023, Vol. 17

Issue (5): 939-956 https://doi.org/10.1007/s11684-023-0987-9

本期目录

Distinct mononuclear diploid cardiac subpopulation with minimal cell–cell communications persists in embryonic and adult mammalian heart

Miaomiao Zhu^1,^2,³, Huamin Liang^1,^2,³, Zhe Zhang¹, Hao Jiang^1,^2,³, Jingwen Pu^1,^2,³, Xiaoyi Hang^1,^2,³, Qian Zhou^1,^2,³, Jiacheng Xiang^1,^2,³, Ximiao He^1,^2,³(

)

¹. Department of Physiology, School of Basic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China
². Center for Genomics and Proteomics Research, School of Basic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China
³. Hubei Key Laboratory of Drug Target Research and Pharmacodynamic Evaluation, Huazhong University of Science and Technology, Wuhan 430030, China

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Abstract：

A small proportion of mononuclear diploid cardiomyocytes (MNDCMs), with regeneration potential, could persist in adult mammalian heart. However, the heterogeneity of MNDCMs and changes during development remains to be illuminated. To this end, 12 645 cardiac cells were generated from embryonic day 17.5 and postnatal days 2 and 8 mice by single-cell RNA sequencing. Three cardiac developmental paths were identified: two switching to cardiomyocytes (CM) maturation with close CM–fibroblast (FB) communications and one maintaining MNDCM status with least CM–FB communications. Proliferative MNDCMs having interactions with macrophages and non-proliferative MNDCMs (non-pMNDCMs) with minimal cell–cell communications were identified in the third path. The non-pMNDCMs possessed distinct properties: the lowest mitochondrial metabolisms, the highest glycolysis, and high expression of Myl4 and Tnni1. Single-nucleus RNA sequencing and immunohistochemical staining further proved that the Myl4⁺Tnni1⁺ MNDCMs persisted in embryonic and adult hearts. These MNDCMs were mapped to the heart by integrating the spatial and single-cell transcriptomic data. In conclusion, a novel non-pMNDCM subpopulation with minimal cell–cell communications was unveiled, highlighting the importance of microenvironment contribution to CM fate during maturation. These findings could improve the understanding of MNDCM heterogeneity and cardiac development, thus providing new clues for approaches to effective cardiac regeneration.

Key words： mononuclear diploid cardiomyocytes cell–cell communication cardiac fibroblast single-cell RNA sequencing cardiac regeneration

收稿日期: 2022-07-31 出版日期: 2023-12-07

Corresponding Author(s): Ximiao He

引用本文:

. [J]. Frontiers of Medicine, 2023, 17(5): 939-956.
Miaomiao Zhu, Huamin Liang, Zhe Zhang, Hao Jiang, Jingwen Pu, Xiaoyi Hang, Qian Zhou, Jiacheng Xiang, Ximiao He. Distinct mononuclear diploid cardiac subpopulation with minimal cell–cell communications persists in embryonic and adult mammalian heart. Front. Med., 2023, 17(5): 939-956.

链接本文:

https://academic.hep.com.cn/fmd/CN/10.1007/s11684-023-0987-9
https://academic.hep.com.cn/fmd/CN/Y2023/V17/I5/939

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