Abstract：The biomimetic apatite coating process was adopted to modify poly(L-lactide) (PLLA) surfaces with osteoblasts-like cell compatibility. The apatite coating was formed on the pre-hydrolyzed PLLA film and scaffold surfaces by incubating in simulated body fluid (SBF). Scanning electron microscopy and energy dispersive X-ray analyzer were utilized to characterize the composition and the structure of the apatite coating. The cytocompatibility of the modified PLLA films was investigated by testing osteoblast-like attachment, proliferation, alkaline phosphatase (ALP) activity, and cell cycle. Subsequently, the modified PLLA scaffolds were co-cultured with the osteoblasts-like in vitro and subcutaneously implanted into nude mice. The experimental results showed that the formed apatite had a nano-sized particle and matrix configuration. The surface modification of PLLA with apatite coating significantly promoted osteoblast-like compatibility. After a four-week culture in vivo, no significant inflammatory signs were observed in the implanted regions and osteoblast-like congeries with bone-like structure began to form in the scaffolds. The positive results of this study suggest a good way to produce desirable PLLA biomaterials for bone tissue engineering.
出版日期: 2007-06-05
引用本文:
. Formation of bone-like apatite on poly(L-lactide) to improve osteoblast-like compatibility in vitro and in vivo[J]. Frontiers of Materials Science in China, 2007, 1(2): 140-146.
JIAO Yanpeng, LIU Zonghua, ZHOU Changren, CUI Fuzhai. Formation of bone-like apatite on poly(L-lactide) to improve osteoblast-like compatibility in vitro and in vivo. Front. Mater. Sci., 2007, 1(2): 140-146.
