In vivo bone regeneration with injectable chitosan/hydroxyapatite/collagen composites and mesenchymal stem cells
In vivo bone regeneration with injectable chitosan/hydroxyapatite/collagen composites and mesenchymal stem cells
Zhi HUANG1, Yan CHEN2, Qing-Ling FENG1(), Wei ZHAO3, Bo YU4(), Jing TIAN4, Song-Jian LI4, Bo-Miao LIN5
1. Department of Materials Science and Engineering, Tsinghua University, Beijing 100084, China; 2. Department of Ultrasonic Diagnosis, Zhujiang Hospital of Southern Medical University, Guangzhou 510282, China; 3. Institute of High Energy Physics, Chinese Academy of Sciences, Beijing 100049, China; 4. Department of Orthopedics, Zhujiang Hospital of Southern Medical University, Guangzhou 510282, China; 5. Department of Radiology, Zhujiang Hospital of Southern Medical University, Guangzhou 510282, China
For reconstruction of irregular bone defects, injectable biomaterials are more appropriate than the preformed biomaterials. We herein develop a biomimetic in situ-forming composite consisting of chitosan (CS) and mineralized collagen fibrils (nHAC), which has a complex hierarchical structure similar to natural bone. The CS/nHAC composites with or without mesenchymal stem cells (MSCs) are injected into cancellous bone defects at the distal end of rabbit femurs. Defects are assessed by radiographic, histological diagnosis and Raman microscopy until 12 weeks. The results show that MSCs improve the biocompatibility of CS/nHAC composites and enhance new bone formation in vivo at 12 weeks. It can be concluded that the injectable CS/nHAC composites combined with MSCs may be a novel method for reconstruction of irregular bone defects.
. In vivo bone regeneration with injectable chitosan/hydroxyapatite/collagen composites and mesenchymal stem cells[J]. Frontiers of Materials Science, 2011, 5(3): 301-310.
Zhi HUANG, Yan CHEN, Qing-Ling FENG, Wei ZHAO, Bo YU, Jing TIAN, Song-Jian LI, Bo-Miao LIN. In vivo bone regeneration with injectable chitosan/hydroxyapatite/collagen composites and mesenchymal stem cells. Front Mater Sci, 2011, 5(3): 301-310.
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