Microbial-inspired self-healing of concrete cracks by sodium silicate-coated recycled concrete aggregates served as bacterial carrier
Jing XU1, Xianzhi WANG1, Wu YAO1, Anna A. KULMINSKAYA2, Surendra P. SHAH3
1. Key Laboratory of Advanced Civil Engineering Materials (Ministry of Education), School of Materials Science and Engineering, Tongji University, Shanghai 201804, China 2. Petersburg Nuclear Physics Institute Named by B.P. Konstantinov of National Research Centre, Kurchatov Institute, Gatchina 188300, Russia 3. Center for Advanced Construction Materials, University of Texas at Arlington, Arlington, TX 76019, USA
Microbially induced carbonate precipitation (MICP) is a promising technique for the autonomous healing of concrete cracks. In this study, the effect of pH on MICP was investigated. The results indicate that the MICP process was inhibited when the pH was higher than 11. Both vaterite and calcite were produced when the pH was < 8, whereas only calcite was produced when the pH was > 8. Recycled concrete aggregates (RCA) coated with sodium silicate have been proposed as protective carriers for microbial healing agents. Although the presence of the coated RCA resulted in a loss of the splitting tension strength of the concrete, the loaded healing agents were highly efficient in self-healing cracks. Concrete incorporated with 20% RCA loaded with healing agents exhibited the best self-healing performance. When the initial crack widths were between 0.3 and 0.4 mm, the 7-d mean healing rate was approximately 90%. At 28 d, the crack area filling ratio was 86.4%, while its water tightness recovery ratio was 74.4% and 29.8%, respectively, for rapid and slow absorption. This study suggests that RCA coated with sodium silicate is an effective method for packaging microbial healing agents and has great potential for developing cost-effective self-healing concrete.
. [J]. Frontiers of Structural and Civil Engineering, 2024, 18(1): 14-29.
Jing XU, Xianzhi WANG, Wu YAO, Anna A. KULMINSKAYA, Surendra P. SHAH. Microbial-inspired self-healing of concrete cracks by sodium silicate-coated recycled concrete aggregates served as bacterial carrier. Front. Struct. Civ. Eng., 2024, 18(1): 14-29.
Y Zhang, S Q Zhang, X Jiang, Q Chen, Z W Jiang, J W Ju, M Bauchy. Insights into the thermal effect on the fracture toughness of calcium silicate hydrate grains: A reactive molecular dynamics study. Cement and Concrete Composites, 2022, 134: 104824 https://doi.org/10.1016/j.cemconcomp.2022.104824
2
Y Zhang, J W Ju, H H Zhu, Z G Yan. A novel multi-scale model for predicting the thermal damage of hybrid fiber-reinforced concrete. International Journal of Damage Mechanics, 2020, 29(1): 19–44 https://doi.org/10.1177/1056789519831554
3
H H Zhu, Q Chen, Z G Yan, J W Ju, S Zhou. Micromechanical models for saturated concrete repaired by the electrochemical deposition method. Materials and Structures, 2014, 47(6): 1067–1082 https://doi.org/10.1617/s11527-013-0115-4
4
Z G Yan, Q Chen, H H Zhu, J W Ju, S Zhou, Z W Jiang. A multi-phase micromechanical model for unsaturated concrete repaired using the electrochemical deposition method. International Journal of Solids and Structures, 2013, 50(24): 3875–3885 https://doi.org/10.1016/j.ijsolstr.2013.07.020
5
H H Zhu, S Zhou, Z G Yan, J W Ju, Q Chen. A two-dimensional micromechanical damage-healing model on microcrack-induced damage for microcapsule-enabled self-healing cementitious composites under tensile loading. International Journal of Damage Mechanics, 2015, 24(1): 95–115 https://doi.org/10.1177/1056789514522503
6
S Zhou, H H Zhu, J W Ju, Z G Yan, Q Chen. Modeling microcapsule-enabled self-healing cementitious composite materials using discrete element method. International Journal of Damage Mechanics, 2017, 26(2): 340–357 https://doi.org/10.1177/1056789516688835
7
J Xu, W Yao. Multiscale mechanical quantification of self-healing concrete incorporating non-ureolytic bacteria-based healing agent. Cement and Concrete Research, 2014, 64(1): 1–10 https://doi.org/10.1016/j.cemconres.2014.06.003
8
J Xu, Y Tang, X Wang, Z Wang, W Yao. Application of ureolysis-based microbial CaCO3 precipitation in self-healing of concrete and inhibition of reinforcement corrosion. Construction & Building Materials, 2020, 265: 120364 https://doi.org/10.1016/j.conbuildmat.2020.120364
9
J Xu, X Wang, B Wang. Biochemical process of ureolysis-based microbial CaCO3 precipitation and its application in self-healing concrete. Applied Microbiology and Biotechnology, 2018, 102(7): 3121–3132 https://doi.org/10.1007/s00253-018-8779-x
10
X Y Zhuang, S Zhou. The prediction of self-healing capacity of bacteria-based concrete using machine learning approaches. Computers, Materials & Continua, 2019, 59(1): 57–77 https://doi.org/10.32604/cmc.2019.04589
11
Q Chen, L J Xie, A L Huang, B Li, Y X Sun, Z W Jiang, W T Li, H H Zhu. Healing of concrete cracks by in-situ synthesis of ettringite induced by electric field. Construction & Building Materials, 2022, 352: 128685 https://doi.org/10.1016/j.conbuildmat.2022.128685
12
E Boquet, A Boronat, A Ramos-Cormenzana. Production of Calcite (Calcium Carbonate) Crystals by Soil Bacteria is a General Phenomenon. Nature, 1973, 246(5434): 527–529 https://doi.org/10.1038/246527a0
13
F Hammes, W Verstraete. Key roles of pH and calcium metabolism in microbial carbonate precipitation. Reviews in Environmental Science and Biotechnology, 2002, 1(1): 3–7 https://doi.org/10.1023/A:1015135629155
14
M J Castro-Alonso, L E Montanez-Hernandez, M A Sanchez-Munoz, M R M Franco, R Narayanasamy, N Balagurusamy. Microbially induced calcium carbonate precipitation (MICP) and its potential in bioconcrete: Microbiological and molecular concepts. Frontiers in Materials, 2019, 6: 126 https://doi.org/10.3389/fmats.2019.00126
15
B Lothenbach, G Le Saout, E Gallucci, K Scrivener. Influence of limestone on the hydration of Portland cements. Cement and Concrete Research, 2008, 38(6): 848–860 https://doi.org/10.1016/j.cemconres.2008.01.002
16
L Basheer, J Kropp, D J Cleland. Assessment of the durability of concrete from its permeation properties: A review. Construction & Building Materials, 2001, 15(2−3): 93–103 https://doi.org/10.1016/S0950-0618(00)00058-1
17
L Jiang, G Jia, Y Wang, Z Li. Optimization of Sporulation and Germination Conditions of Functional Bacteria for Concrete Crack-Healing and Evaluation of their Repair Capacity. ACS Applied Materials & Interfaces, 2020, 12(9): 10938–10948 https://doi.org/10.1021/acsami.9b21465
18
Y C Ersan, E Hernandez-Sanabria, N Boon, N de Belie. Enhanced crack closure performance of microbial mortar through nitrate reduction. Cement and Concrete Composites, 2016, 70: 159–170 https://doi.org/10.1016/j.cemconcomp.2016.04.001
19
T Zheng, Y Su, X Zhang, H Zhou, C Qian. Effect and mechanism of encapsulation-based spores on self-healing concrete at different curing ages. ACS Applied Materials & Interfaces, 2020, 12(47): 52415–52432 https://doi.org/10.1021/acsami.0c16343
20
J Xu, X Wang. Self-healing of concrete cracks by use of bacteria-containing low alkali cementitious material. Construction & Building Materials, 2018, 167: 1–14 https://doi.org/10.1016/j.conbuildmat.2018.02.020
21
A J Phillips, R Gerlach, E Lauchnor, A C Mitchell, A B Cunningham, L Spangler. Engineered applications of ureolytic biomineralization: A review. Biofouling, 2013, 29(6): 715–733 https://doi.org/10.1080/08927014.2013.796550
22
J Wang, A Mignon, G Trenson, S Van Vlierberghe, N Boon, N De Belie. A chitosan based pH-responsive hydrogel for encapsulation of bacteria for self-sealing concrete. Cement and Concrete Composites, 2018, 93: 309–322 https://doi.org/10.1016/j.cemconcomp.2018.08.007
23
H M Jonkers, A Thijssen, G Muyzer, O Copuroglu, E Schlangen. Application of bacteria as self-healing agent for the development of sustainable concrete. Ecological Engineering, 2010, 36(2): 230–235 https://doi.org/10.1016/j.ecoleng.2008.12.036
24
Y H Tang, J Xu. Application of microbial precipitation in self-healing concrete: A review on the protection strategies for bacteria. Construction & Building Materials, 2021, 306: 124950 https://doi.org/10.1016/j.conbuildmat.2021.124950
25
S Han, I Jang, E K Choi, W Park, C Yi, N Chung. Bacterial self-healing performance of coated expanded clay in concrete. Journal of Environmental Engineering, 2020, 146(7): 04020072 https://doi.org/10.1061/(ASCE)EE.1943-7870.0001713
26
H Yuan, Q Zhang, X Hu, M Wu, Y Zhao, Y Feng, D Shen. Application of zeolite as a bacterial carrier in the self-healing of cement mortar cracks. Construction & Building Materials, 2022, 331: 127324 https://doi.org/10.1016/j.conbuildmat.2022.127324
27
L Tan, B Reeksting, V Ferrandiz-Mas, A Heath, S Gebhard, K Paine. Effect of carbonation on bacteria-based self-healing of cementitious composites. Construction & Building Materials, 2020, 257: 119501 https://doi.org/10.1016/j.conbuildmat.2020.119501
28
M Alazhari, T Sharma, A Heath, R Cooper, K Paine. Application of expanded perlite encapsulated bacteria and growth media for self-healing concrete. Construction & Building Materials, 2018, 160: 610–619 https://doi.org/10.1016/j.conbuildmat.2017.11.086
29
L Jiang, G Jia, C Jiang, Z Li. Sugar-coated expanded perlite as a bacterial carrier for crack-healing concrete applications. Construction & Building Materials, 2020, 232: 117222 https://doi.org/10.1016/j.conbuildmat.2019.117222
30
J Zhang, C Zhao, A Zhou, C Yang, L Zhao, Z Li. Aragonite formation induced by open cultures of microbial consortia to heal cracks in concrete: Insights into healing mechanisms and crystal polymorphs. Construction & Building Materials, 2019, 224: 815–822 https://doi.org/10.1016/j.conbuildmat.2019.07.129
31
Y Su, F Li, Z He, C Qian. Artificial aggregates could be a potential way to realize microbial self-healing concrete: An example based on modified ceramsite. Journal of Building Engineering, 2021, 35: 102082 https://doi.org/10.1016/j.jobe.2020.102082
32
X Wang, J Xu, Z Wang, W Yao. Use of recycled concrete aggregates as carriers for self-healing of concrete cracks by bacteria with high urease activity. Construction & Building Materials, 2022, 337: 127581 https://doi.org/10.1016/j.conbuildmat.2022.127581
33
Y Zhang, S Zhang, W Zhao, X Jiang, Y Chen, J Hou, Y Wang, Z Yan, H Zhu. Influence of multi-scale fiber on residual compressive properties of a novel rubberized concrete subjected to elevated temperatures. Journal of Building Engineering, 2023, 65: 105750 https://doi.org/10.1016/j.jobe.2022.105750
34
C S Poon, Z H Shui, L Lam. Effect of microstructure of ITZ on compressive strength of concrete prepared with recycled aggregates. Construction & Building Materials, 2004, 18(6): 461–468 https://doi.org/10.1016/j.conbuildmat.2004.03.005
35
F Huang, S Zhou. A Review of lightweight self-healing concrete. Materials (Basel), 2022, 15(21): 7572 https://doi.org/10.3390/ma15217572
36
X Jiang, R Xiao, Y Bai, B S Huang, Y T Ma. Influence of waste glass powder as a supplementary cementitious material (SCM) on physical and mechanical properties of cement paste under high temperatures. Journal of Cleaner Production, 2022, 340: 130778 https://doi.org/10.1016/j.jclepro.2022.130778
37
X Jiang, Y Zhang, Y Zhang, J Ma, R Xiao, F Guo, Y Bai, B Huang. Influence of size effect on the properties of slag and waste glass-based geopolymer paste. Journal of Cleaner Production, 2023, 383: 135428 https://doi.org/10.1016/j.jclepro.2022.135428
38
X Jiang, R Xiao, M M Zhang, W Hu, Y Bai, B S Huang. A laboratory investigation of steel to fly ash-based geopolymer paste bonding behavior after exposure to elevated temperatures. Construction & Building Materials, 2020, 254: 119267 https://doi.org/10.1016/j.conbuildmat.2020.119267
39
R A Khushnood, Z A Qureshi, N Shaheen, S Ali. Bio-mineralized self-healing recycled aggregate concrete for sustainable infrastructure. Science of the Total Environment, 2020, 703: 135007 https://doi.org/10.1016/j.scitotenv.2019.135007
40
H Mefteh, O Kebaïli, H Oucief, L Berredjem, N Arabi. Influence of moisture conditioning of recycled aggregates on the properties of fresh and hardened concrete. Journal of Cleaner Production, 2013, 54: 282–288 https://doi.org/10.1016/j.jclepro.2013.05.009
41
C Shi, Y Li, J Zhang, W Li, L Chong, Z Xie. Performance enhancement of recycled concrete aggregate—A review. Journal of Cleaner Production, 2016, 112: 466–472 https://doi.org/10.1016/j.jclepro.2015.08.057
42
A OktariV SupriatinM KamalH Syafrullah. The bacterial endospore stain on schaeffer fulton using variation of methylene blue solution. In: 3rd International Seminar on Mathematics, Science, and Computer Science Education (MSCEIS). Bandung: IOP Publishing Ltd, 2016
43
S K Ramachandran, V Ramachandran, S S Bang. Remediation of concrete using microorganisms. ACI Materials Journal, 2001, 98(1): 3–9
44
Whiffin V S. Microbial CaCO3 precipitation for the production of biocement. Dissertation for the Doctoral Degree. Murdoch: Murdoch University, 2004
W de Muynck, D Debrouwer, N de Belie, W Verstraete. Bacterial carbonate precipitation improves the durability of cementitious materials. Cement and Concrete Research, 2008, 38(7): 1005–1014 https://doi.org/10.1016/j.cemconres.2008.03.005
Y C Ersan, H Verbruggen, I de Graeve, W Verstraete, N de Belie, N Boon. Nitrate reducing CaCO3 precipitating bacteria survive in mortar and inhibit steel corrosion. Cement and Concrete Research, 2016, 83: 19–30 https://doi.org/10.1016/j.cemconres.2016.01.009
49
J Xu, Y Du, Z Jiang, A She. Effects of calcium source on biochemical properties of microbial CaCO3 precipitation. Frontiers in Microbiology, 2015, 6: 1366 https://doi.org/10.3389/fmicb.2015.01366
50
W de Muynck, N de Belie, W Verstraete. Microbial carbonate precipitation in construction materials: A review. Ecological Engineering, 2010, 36(2): 118–136 https://doi.org/10.1016/j.ecoleng.2009.02.006
51
A C Mitchell, A Phillips, L Schultz, S Parks, L Spangler, A B Cunningham, R Gerlach. Microbial CaCO3 mineral formation and stability in an experimentally simulated high pressure saline aquifer with supercritical CO2. International Journal of Greenhouse Gas Control, 2013, 15: 86–96 https://doi.org/10.1016/j.ijggc.2013.02.001
52
E G Lauchnor, D M Topp, A E Parker, R Gerlach. Whole cell kinetics of ureolysis by Sporosarcina pasteurii. Journal of Applied Microbiology, 2015, 118(6): 1321–1332 https://doi.org/10.1111/jam.12804
53
J Tourney, B T Ngwenya. Bacterial extracellular polymeric substances (EPS) mediate CaCO3 morphology and polymorphism. Chemical Geology, 2009, 262(3−4): 138–146 https://doi.org/10.1016/j.chemgeo.2009.01.006
54
F C Meldrum, H Cölfen. Controlling mineral morphologies and structures in biological and synthetic systems. Chemical Reviews, 2008, 108(11): 4332–4432 https://doi.org/10.1021/cr8002856
55
N Spanos, P G Koutsoukos. The transformation of vaterite to calcite: effect of the conditions of the solutions in contact with the mineral phase. Journal of Crystal Growth, 1998, 191(4): 783–790 https://doi.org/10.1016/S0022-0248(98)00385-6
56
G Nehrke, P Van Cappellen. Framboidal vaterite aggregates and their transformation into calcite: A morphological study. Journal of Crystal Growth, 2006, 287(2): 528–530 https://doi.org/10.1016/j.jcrysgro.2005.11.080
57
C Rodriguez-Navarro, M Rodriguez-Gallego, Chekroun K Ben, Maria T Gonzalez-Muñoz. Conservation of ornamental stone by myxococcus xanthus—Induced carbonate biomineralization. Applied and Environmental Microbiology, 2003, 69(4): 2182–2193 https://doi.org/10.1128/AEM.69.4.2182-2193.2003
58
H Kawaguchi, H Hirai, K Sakai, S Sera, T Nakajima, Y Ebisawa, K Koyama. Crystallization of inorganic compounds in polymer solutions. Part I: Control of shape and form of calcium carbonate. Colloid & Polymer Science, 1992, 270(12): 1176–1181 https://doi.org/10.1007/BF01095057
59
C Lv, C S Tang, C Zhu, W Q Li, T Y Chen, L Zhao, X H Pan. Environmental dependence of microbially induced calcium carbonate crystal precipitations: experimental evidence and insights. Journal of Geotechnical and Geoenvironmental Engineering, 2022, 148(7): 04022050 https://doi.org/10.1061/(ASCE)GT.1943-5606.0002827
60
J Xu, Y Tang, X Wang. A correlation study on optimum conditions of microbial precipitation and prerequisites for self-healing concrete. Process Biochemistry (Barking, London, England), 2020, 94: 266–272 https://doi.org/10.1016/j.procbio.2020.04.028
61
T Ozbakkaloglu, A Gholampour, T Xie. Mechanical and durability properties of recycled aggregate concrete: Effect of recycled aggregate properties and content. Journal of Materials in Civil Engineering, 2018, 30(2): 04017275 https://doi.org/10.1061/(ASCE)MT.1943-5533.0002142
62
N de Belie, E Gruyaert, A Al-Tabbaa, P Antonaci, C Baera, D Bajare, A Darquennes, R Davies, L Ferrara, T Jefferson, C Litina, B Miljevic, A Otlewska, J Ranogajec, M Roig-Flores, K Paine, P Lukowski, P Serna, J M Tulliani, S Vucetic, J Wang, H M Jonkers. A Review of self-healing concrete for damage management of structures. Advanced Materials Interfaces, 2018, 5(17): 1800074 https://doi.org/10.1002/admi.201800074
63
M Mirshahmohammad, H Rahmani, M Maleki-Kakelar, A Bahari. Effect of sustained service loads on the self-healing and corrosion of bacterial concretes. Construction & Building Materials, 2022, 322: 126423 https://doi.org/10.1016/j.conbuildmat.2022.126423
64
X H Li, R T Liu, S C Li, C Y Zhang, J Yan, Y K Liu, X B Sun, P S Su. Properties and mechanism of self-healing cement paste containing microcapsule under different curing conditions. Construction & Building Materials, 2022, 357: 129410 https://doi.org/10.1016/j.conbuildmat.2022.129410
65
L Hanžič, L Kosec, I Anžel. Capillary absorption in concrete and the Lucas–Washburn equation. Cement and Concrete Composites, 2010, 32(1): 84–91 https://doi.org/10.1016/j.cemconcomp.2009.10.005
D Benavente, P Lock, García Del Cura M Ángeles, S Ordóñez. Predicting the capillary imbibition of porous rocks from microstructure. Transport in porous media, 2002, 49(1): 59–76
68
N M Alderete, Zaccardi Y A Villagrán, Belie N De. Physical evidence of swelling as the cause of anomalous capillary water uptake by cementitious materials. Cement and Concrete Research, 2019, 120: 256–266 https://doi.org/10.1016/j.cemconres.2019.04.001
69
T Zheng, Y Su, C Qian, H Zhou. Low alkali sulpho-aluminate cement encapsulated microbial spores for self-healing cement-based materials. Biochemical Engineering Journal, 2020, 163: 107756 https://doi.org/10.1016/j.bej.2020.107756