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Protein & Cell

ISSN 1674-800X

ISSN 1674-8018(Online)

CN 11-5886/Q

邮发代号 80-984

2019 Impact Factor: 10.164

Protein & Cell  2023, Vol. 14 Issue (4): 304-308   https://doi.org/10.1093/procel/pwac014
  本期目录
Enhancing prime editing efficiency and flexibility with tethered and split pegRNAs
Ying Feng1,2, Siyuan Liu2, Qiqin Mo2, Pengpeng Liu3, Xiao Xiao1(), Hanhui Ma2()
1. School of Biotechnology, East China University of Science and Technology, Shanghai 200237, China
2. Gene Editing Center, School of Life Science and Technology, ShanghaiTech University, Shanghai 200135, China; School of Biotechnology, East China University of Science and Technology, Shanghai 200237, China
3. Department of Molecular, Cell and Cancer Biology, University of Massachusetts Medical School, Worcester, MA 01655, USA
 全文: PDF(1136 KB)  
出版日期: 2023-04-28
Corresponding Author(s): Xiao Xiao,Hanhui Ma   
 引用本文:   
. [J]. Protein & Cell, 2023, 14(4): 304-308.
Ying Feng, Siyuan Liu, Qiqin Mo, Pengpeng Liu, Xiao Xiao, Hanhui Ma. Enhancing prime editing efficiency and flexibility with tethered and split pegRNAs. Protein Cell, 2023, 14(4): 304-308.
 链接本文:  
https://academic.hep.com.cn/pac/CN/10.1093/procel/pwac014
https://academic.hep.com.cn/pac/CN/Y2023/V14/I4/304
1 AV Anzalone, XD Gao, CJ Podracky et al. Programmable deletion, replacement, integration and inversion of large DNA sequences with twin prime editing. Nat Biotechnol 2022;40:731–740.
https://doi.org/10.1038/s41587-021-01133-w
2 AV Anzalone, PB Randolph, JR Davis et al. Search-and-replace genome editing without double-strand breaks or donor DNA. Nature 2019;576:149–157.
https://doi.org/10.1038/s41586-019-1711-4
3 S Bae, J Park, JS. Kim Cas-OFFinder: a fast and versatile algorithm that searches for potential off-target sites of Cas9 RNA-guided endonucleases. Bioinformatics 2014;30:1473–1475.
https://doi.org/10.1093/bioinformatics/btu048
4 PJ Chen, JA Hussmann, J Yan et al. Enhanced prime editing systems by manipulating cellular determinants of editing outcomes. Cell 2021;184:5635–5652.e29.
https://doi.org/10.1016/j.cell.2021.09.018
5 J Choi, W Chen, CC Suiter et al. Precise genomic deletions using paired prime editing. Nat Biotechnol 2022;40:218–226.
https://doi.org/10.1038/s41587-021-01025-z
6 MA Convery, S Rowsell, NJ Stonehouse et al. Crystal structure of an RNA aptamer-protein complex at 2.8 A resolution. Nat Struct Biol 1998;5:133–139.
https://doi.org/10.1038/nsb0298-133
7 M Kosicki, K Tomberg, A. Bradley Repair of double-strand breaks induced by CRISPR-Cas9 leads to large deletions and complex rearrangements. Nat Biotechnol 2018;36:765–771.
https://doi.org/10.1038/nbt.4192
8 MJ Landrum, JM Lee, M Benson et al. ClinVar: public archive of interpretations of clinically relevant variants. Nucleic Acids Res 2016;44:D862–D868.
https://doi.org/10.1093/nar/gkv1222
9 Q Lin, Y Zong, C Xue et al. Prime genome editing in rice and wheat. Nat Biotechnol 2020;38:582–585.
https://doi.org/10.1038/s41587-020-0455-x
10 JL Litke, SR. Jaffrey Highly efficient expression of circular RNA aptamers in cells using autocatalytic transcripts. Nat Biotechnol 2019;37:667–675.
https://doi.org/10.1038/s41587-019-0090-6
11 Y Liu, G Yang, S Huang et al. Enhancing prime editing by Csy4-mediated processing of pegRNA. Cell Res 2021;31:1134–1136.
https://doi.org/10.1038/s41422-021-00520-x
12 H Ma, LC Tu, A Naseri et al. CRISPR-Cas9 nuclear dynamics and target recognition in living cells. J Cell Biol 2016;214:529–537.
https://doi.org/10.1083/jcb.201604115
13 H Ma, LC Tu, A Naseri et al. CRISPR-Sirius: RNA scaffolds for signal amplification in genome imaging. Nat Methods 2018;15:928–931.
https://doi.org/10.1038/s41592-018-0174-0
14 JW Nelson, PB Randolph, SP Shen et al. Engineered pegRNAs improve prime editing efficiency. Nat Biotechnol 2022;40:402–410.
https://doi.org/10.1038/s41587-021-01039-7
15 BZ Stanton, EJ Chory, GR. Crabtree Chemically induced proximity in biology and medicine. Science 2018;359:6380.
https://doi.org/10.1126/science.aao5902
16 MO Urbanek, P Galka-Marciniak, M Olejniczak et al. RNA imaging in living cells—methods and applications. RNA Biol 2014;11:1083–1095.
https://doi.org/10.4161/rna.35506
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