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Quantitative Biology

ISSN 2095-4689

ISSN 2095-4697(Online)

CN 10-1028/TM

邮发代号 80-971

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Quantitative Biology  2014, Vol. 2 Issue (2): 59-70   https://doi.org/10.1007/s40484-014-0030-x
  REVIEW 本期目录
Target specificity of the CRISPR-Cas9 system
Xuebing Wu1,2,Andrea J. Kriz3,Phillip A. Sharp1,3,*()
1. David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
2. Computational and Systems Biology Graduate Program, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
3. Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
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Abstract：

The CRISPR-Cas9 system, naturally a defense mechanism in prokaryotes, has been repurposed as an RNA-guided DNA targeting platform. It has been widely used for genome editing and transcriptome modulation, and has shown great promise in correcting mutations in human genetic diseases. Off-target effects are a critical issue for all of these applications. Here we review the current status on the target specificity of the CRISPR-Cas9 system.
Key words： CRISPR    Cas9    target specificity    off-targets    genome engineering
收稿日期: 2014-06-30      出版日期: 2014-12-04
Corresponding Author(s): Phillip A. Sharp   
 引用本文:   
. [J]. Quantitative Biology, 2014, 2(2): 59-70.
Xuebing Wu, Andrea J. Kriz, Phillip A. Sharp. Target specificity of the CRISPR-Cas9 system. Quant. Biol., 2014, 2(2): 59-70.
 链接本文:  
https://academic.hep.com.cn/qb/CN/10.1007/s40484-014-0030-x
https://academic.hep.com.cn/qb/CN/Y2014/V2/I2/59
Fig.1  
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