Recent advances and application in whole-genome multiple displacement amplification

doi:10.1007/s40484-020-0217-2

Quantitative Biology

2020, Vol. 8

Issue (4): 279-294 https://doi.org/10.1007/s40484-020-0217-2

本期目录

Recent advances and application in whole-genome multiple displacement amplification

Naiyun Long, Yi Qiao, Zheyun Xu, Jing Tu(

), Zuhong Lu(

)

State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China

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Abstract：

Background: The extremely small amount of DNA in a cell makes it difficult to study the whole genome of single cells, so whole-genome amplification (WGA) is necessary to increase the DNA amount and enable downstream analyses. Multiple displacement amplification (MDA) is the most widely used WGA technique.

Results: Compared with amplification methods based on PCR and other methods, MDA renders high-quality DNA products and better genome coverage by using phi29 DNA polymerase. Moreover, recently developed advanced MDA technologies such as microreactor MDA, emulsion MDA, and micro-channel MDA have improved amplification uniformity. Additionally, the development of other novel methods such as TruePrime WGA allows for amplification without primers.

Conclusion: Here, we reviewed a selection of recently developed MDA methods, their advantages over other WGA methods, and improved MDA-based technologies, followed by a discussion of future perspectives. With the continuous development of MDA and the successive update of detection technologies, MDA will be applied in increasingly more fields and provide a solid foundation for scientific research.

Key words： whole genome amplification multiple displacement amplification improved MDA-based approaches

收稿日期: 2020-03-26 出版日期: 2020-12-24

Corresponding Author(s): Jing Tu,Zuhong Lu

引用本文:

. [J]. Quantitative Biology, 2020, 8(4): 279-294.
Naiyun Long, Yi Qiao, Zheyun Xu, Jing Tu, Zuhong Lu. Recent advances and application in whole-genome multiple displacement amplification. Quant. Biol., 2020, 8(4): 279-294.

链接本文:

https://academic.hep.com.cn/qb/CN/10.1007/s40484-020-0217-2
https://academic.hep.com.cn/qb/CN/Y2020/V8/I4/279

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