Soil amendment strategies determining microbial community composition and their assembly processes in a continuously cropped soil
Hongkai Liao1, Chunli Zheng2, Juan Li1, Jian Long1, Yaying Li3,4()
1. Guizhou Provincial Key Laboratory of Mountain Environment, Guizhou Normal University, Guiyang 550001, China 2. The Key Laboratory of Environmental Pollution Monitoring and Disease Control, Ministry of Education, Department of Toxicology, School of Public Health, Guizhou Medical University, Guiyang 550025, China 3. Key Laboratory of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, China 4. Key Laboratory of Urban Environmental Processes and Pollution Control, Ningbo (Beilun) Zhongke Haixi Industry Technology Innovation Center, Ningbo 315800, China
● Phyla Proteobacteria and Bacteroidetes dominated in the rhizosphere of tomatoes under RSD amendment.
● Stochastic processes dominated in bacterial community assembly for biochar and CF amendments.
● It is important to monitor and manage soil conditions before planting after RSD amendment.
Reductive soil disinfestation (RSD) is an important tool for sustainable agricultural productivity. However, the differences in soil bacterial communities and their community assembly processes among RSD and other treatment strategies (e.g., biochar and chemical fumigation) are still subject to open questions. In this study, soils subjected to various treatments–un-treated control (CK), chemical soil fumigation with CaCN2 (CF), 1% biochar (1%B), 3% biochar (3%B), and reductive soil disinfestation (RSD) are investigated. Soil samples were collected, incubated, and then used for growth of tomato plants. The Sloan neutral community model indicates that stochastic processes dominate in bacterial community assembly for both biochar and CF amendments. In contrast, this work shows that RSD treatment can have a strong impact on soil bacterial community composition. The relative abundance of Firmicutes increased during unplanted soil incubation, whereas Proteobacteria and Bacteroidetes dominated in the rhizosphere after planting of tomatoes. Normalized stochasticity ratio reveals that deterministic selection played an important role in the bacterial assembly under RSD amendment. We found that RSD amendment yielded lower biomass than that for other treatments after 28 days of tomato growth. Our results suggest that although RSD treatment has great potential to rebuild soil bacterial ecology by shaping bacterial communities and their assembly processes, it is important to monitor and manage soil conditions (e.g., soil nutrients or physical properties) before planting to ensure plant productivity.
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