SRAP marker reveals genetic diversity in tartary
buckwheat in China

doi:10.1007/s11703-009-0071-5

Front. Agric. China

2009, Vol. 3

Issue (4) : 383-387 https://doi.org/10.1007/s11703-009-0071-5

Research articles

SRAP marker reveals genetic diversity in tartary buckwheat in China

Yanqin LI¹,Xiaoling FAN¹,Tonglin SHI¹,Quanbin ZHANG¹,Zongwen ZHANG²,

1.Key Laboratory for Chemical Biology and Molecular Engineering of Education, Institute of Biotechnology, Shanxi University, Taiyuan 030006, China; 2.The Chinese Academy of Agricultural Sciences, Beijing 100081, China;

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Abstract Sequence-related amplified polymorphism (SRAP) marker was employed to analyze genetic diversity of 10 accessions of tartary buckwheat selected from a wide geographical area in China. Of the total 30 primer combinations investigated, 26 could amplify clearly and consistently. They produced a total of 285 fragments, of which 235 (82.5%) were polymorphic bands. Among the 26 primer combinations, five could discriminate all the genotypes used in this study. Based on the molecular data, the genetic similarity coefficients varied from 0.61 to 0.78 and calculated using the NTSYSpc published by Nei and Li (1979). The cluster analysis revealed that the 10 accessions were better to be grouped into two major clusters at a similarity level of 0.69. Moreover, the accessions collected from the same province turned out to be grouped in the same cluster, which indicated some geographical relationships. It also proved that the SRAP marker system was useful in identification and genetic diversity analysis of tartary buckwheat.

Keywords genetic diversity NTSYS-pc SRAP marker tartary buckwheat

Issue Date: 05 December 2009

Cite this article:

Zongwen ZHANG,Yanqin LI,Tonglin SHI, et al. SRAP marker reveals genetic diversity in tartary buckwheat in China[J]. Front. Agric. China, 2009, 3(4): 383-387.

URL:

https://academic.hep.com.cn/fag/EN/10.1007/s11703-009-0071-5
https://academic.hep.com.cn/fag/EN/Y2009/V3/I4/383

	Budak H, Shearman R C, Parmaksiz I, Dweikat I(2004). Comparative analysis of seeded andvegetative biotype buffalograsses based on phylogenetic relationshipusing ISSRs, SSRs, RAPDs and SRAPs. TheorAppl Genet, 109: 280–288 doi: 10.1007/s00122-004-1630-z
	Fabjan N, Rode J, Kosir I J, Wang Z, Zhang Z, Kreft I(2003). Tartary buckwheat (Fagopyrum tataricum Gaertn) as a source ofdietary rutin and quercitrin. J Hered, 42: 85–89
	Ferriol M, Pico B, Nuez F(2003). Genetic diversityof a germplasm collection of Cucurbita pepousing SRAP and AFLP markers. Theor ApplGenet, 107: 271–282 doi: 10.1007/s00122-003-1242-z
	Guardia T, Rotelli A E, Juarez A O, Pelzer L E(2001). Anti-inflammatory properties of rutin,quercetin and hesperidin on adjuvant arthritis in rat. Farmaco, 56(9): 683–687 doi: 10.1016/S0014-827X(01)01111-9
	Guo L D, Luo Z R(2006). Genetic relationships of some PCNA persimmons (Diospyros kaki Thunb) from China and Japan revealed bySRAP analysis. Genet Resour Crop Ev, 53: 1597–1603 doi: 10.1007/s10722-005-8717-5
	He J, Klag M J, Whelton P K, Mo J P, Chen J Y, Qian M G, Mo P S, He G Q(1995). Oatsand buckwheat intake and cardiovascular disease risk factors in anethnic minority of China. Am J Clin Nutr, 61: 366–372
	Jiang J, Xing J(1992). Dalianshan region in Sichuan province one of the habitats of tatarybuckwheat. In: Proceedings of the 5th InternationalSymposium on Buckwheat, August 20―26, 1992, Taiyuan, China. Taiwan: AgriculturePublishing House, 17–18
	Li G, Gao M, Yang B, Quiros C F(2003). Gene for gene alignment between the Brassica and Arabidopsis genomes by direct transcriptome mapping. Theor Appl Genet, 107: 168–180
	Li G, Quiros C F(2001). Sequence-related amplified polymorphism (SRAP), a new marker systembased on a simple PCR reaction: its application to mapping and genetagging in Brassica. Theor Appl Genet, 103: 455–461 doi: 10.1007/s001220100570
	Li Y Q, Shi T L, Zhang Z W(2007). Development of microsatellitemarkers from tartary buckwheat. BiotechnolLett, 29: 823–827 doi: 10.1007/s10529-006-9293-2
	Lin Z X, Zhang X L, Nie Y C, He D H, Wu M Q(2003). Constructionof a genetic linkage map for cotton based on SRAP. Chin Sci Bull, 48: 2063–2068 doi: 10.1360/03wc0193
	Nagasawa T, Tabata N, Ito Y, Aiba Y, Nishizawa N, Kitts D D(2003). Dietary G-rutin suppresses glycationin tissue proteins of streptozoticin-induced diabetic rats. Mol Cell Biochem, 252: 141–147 doi: 10.1023/A:1025563519088
	Nei M, Li W(1979). Mathematical model for studying genetic variation in terms of restrictionendonucleases. Proc Nat Acad Sci USA, 76: 5269–5273 doi: 10.1073/pnas.76.10.5269
	NTSYS-pc: Numerical Taxonomyand Multivariate Analysis System. Version2.1 (2000). New York: Exeter Publications
	Oomah B D, Mazza G (1996). Flavonoids and antioxidative activities in buckwheat. J Agric Food Chem, 44: 1746–1750 doi: 10.1021/jf9508357
	Park B J, Park C H(2004). Cytotoxic activities of tartary buckwheat against human cancer cells. Proc 9th Int Symp on Buckwheat at Prague, 665–668
	Qiao L X, Liu H Y, Guo B T, Weng M L, Dai J X, Duan D L, Wang B(2007). Molercular identification of 16 Porphyralines using sequence-related amplified polymorphism markers. Aquat Bot, 87: 203–208 doi: 10.1016/j.aquabot.2007.06.006
	Riaz A, Potter D, Stephen M(2004). Genotyping of peachand nectarine cultivars with SSR and SRAP molecular markers. J Am Soc Hort Sci, 129: 204–211
	Wang J, Liu Z, Fu X, Run M(1992). A clinical observation on the hypoglycemic effect ofXinjiang buckwheat. In: Proceedings ofthe 5th International Symposium on Buckwheat. Taiyuan: China Agricultural Science& Technology Press, 465–467
	Wang Y J, Clayton , Campbell(2007). Tatary buckwheat breeding (Fagopyrum tataricum (L.) Gaertn) throughhybridization with its Rice-Tatary type. Euphytica, 156: 399–405 doi: 10.1007/s10681-007-9389-3

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