Analysis of genetic diversity of maize hybrids
in the regional tests in Sichuan and Southwest China

doi:10.1007/s11703-008-0022-6

Front. Agric. China

2008, Vol. 2

Issue (2) : 162-171 https://doi.org/10.1007/s11703-008-0022-6

Analysis of genetic diversity of maize hybrids in the regional tests in Sichuan and Southwest China

CHEN Fabo¹, YANG Kecheng², RONG Tingzhao², PAN Guangtang²

1.Chongqing Fuling District Agricultural Science Institute; 2.Key Laboratory of Crop Genetic Resources and Improvement, Maize Research Institute, Sichuan Agricultural University

Download: PDF(1063 KB) HTML
Export: BibTeX | EndNote | Reference Manager | ProCite | RefWorks

Abstract In this study, analyses of phenotypic characters, SSR molecular markers and pedigrees were done to study the genetic diversity in 186 maize hybrids that were tested in regional trials in Sichuan and Southwest China. The results showed that there were differences in the variation coefficients of different characteristics, but all of the variation coefficients changed within a narrow range. Sixty pairs of simple sequence repeat (SSR) primer distributed on the ten chromosomes of maize produced stable amplified bands and 608 alleles were detected among the hybrids. The average number of alleles per locus was 10.1 ranging from 3 to 23. The values of polymorphism information content (PIC) for each SSR locus varied from 0.5179 to 0.9256 with an average of 0.7826. The genetic similarities of SSR marker pattern among the 186 hybrids ranged from 0.6067 to 0.9162, with an average of 0.7722. There were 16499 pairs of genetic similarity, in which 96.9% were 0.70000 to 0.9256. The cluster analysis showed that the hybrids could be classified into ten clusters, with 88.2% of the hybrids included in Cluster 4, Cluster 8 and Cluster 10. The analysis of pedigree sources of 51 hybrids showed that 36 hybrids had close genetic relationships with the hybrids developed by the Pioneer Company in the late 1980s and early 1990s in the United States, such as Y78599, Y7865 and Y78698, accounting for 70.58%. Meanwhile, 13 hybrids had close genetic relationships with Y78599, accounting for 8.66%. The genetic similarities of SSR marker pattern among the 51 hybrids ranged from 0.66192 to 0.8799, with an average of 0.7686. There were 1196 pairs of genetic similarity ranged between 0.7000 to 0.8796, accounting for 93.80% of all the genetic similarity pairs. The cluster analysis showed that 88.2% of the 51 hybrids were in Cluster 4, Cluster 8 and Cluster 10, which indicated that similarity was high and genetic diversity narrow among the 186 hybrids. This showed that it is necessary to broaden the genetic basis of breeding germplasm in maize.

Issue Date: 05 June 2008

Cite this article:

CHEN Fabo,YANG Kecheng,RONG Tingzhao, et al. Analysis of genetic diversity of maize hybrids in the regional tests in Sichuan and Southwest China[J]. Front. Agric. China, 2008, 2(2): 162-171.

URL:

https://academic.hep.com.cn/fag/EN/10.1007/s11703-008-0022-6
https://academic.hep.com.cn/fag/EN/Y2008/V2/I2/162

1	Fan X M Tan J Li M S Yang J Y Chen H M 2004 Genetic diversity of Chinesetemperate and exotic tropical, subtropical quality protein maize inbredlines by SSR markersAgric Sci China3(2)94100
2	Fan X M Tan J Zhang S H Li M S Hang Y X Yang J Y Peng Z B Li X H 2003 Heterotic grouping for 25 tropical maizeinbred lines and 4 temperate maize inbred lines by SSR markersActa Agron Sin29(6)835840 (in Chinese)
3	Forrest A T 2004 Background of U.S. hybrid corn II: breeding, climate, andfoodCrop Sci44(2)370380
4	Huang Y Q Li J S 2001 Classificationof heterotic groups with RFLPs among 45 maize inbred linesSci Agric Sin34(3)244250 (in Chinese)
5	James G G Joanne A L Kendall R L Margaret E S Stephen K 2002 SSR variation in importantU.S. maize inbred linesCrop Sci42951957
6	Li X H Fu J H Zhang S H Yuan L X Li M S 2000 Genetic variation of inbredlines of maize detected by SSR markersSciAgric Sin33(2)19 (in Chinese)
7	Li X H Yuan L X Li X H Zhang S H Li M H Li W H 2003 Heterotic grouping of 70 maize inbred lines by SSR markersSci Agri Sin36(6)622627 (in Chinese)
8	Liu K J Goodman M Muse S Smith J S Buckler E John D 2003 Genetic structure and diversity among maize inbred linesas inferred from DNA microsatellitesGenetics165(9)21172128
9	Liu X H Liu W X Zhang Y R Liu Z X Zhang S K Dai J R 2005 Genetic relationship among several maize inbreds revealedby SSR markersMol Plant Breed3(2)17987 (in Chinese)
10	Nei M Li W H 1979 Mathematicalmodel for studying genetic variation in terms of restriction endonucleasesProc Nat Acad Sci7652565273
11	Nie Y X Zhang L Pan G T Rong T Z 2005 Genetic diversity of dominant maize inbred lines revealed by SSRin Sichuan ProvinceMol Plant Breed3(1)4351 (in Chinese)
12	Senior M L Murphy J P Goodman M M Stuber C W 1998 Utility of SSRs for determining genetic similarities and relationshipsin maize using an agarose gel systemCropSci3810881098
13	Wang Y B Zhang Q J Zhu L H 1986 A synthetic analysis and evaluation forthe germplasm base of maize hybrids in Henan ProvinceActa Agric Unive Henanensis20(1)6272 (in Chinese)
14	Wu J F 1983 Review germplasm basis for major maize hybrids in ChinaSci Agric Sin(2)182 (in Chinese)
15	Xia X C Reif J C Melchinger A E Frish M Hoising D A Beck D Pixley K Warburton M L 2005 Genetic diversity among CIMMYT maize inbredlines investigated with SSR marker: II. suptropical, tropical midaltitude,and highland maize inbred lines and their relationships with eliteU.S. and European maizeCrop Sci45(6)25732582. doi:10.2135/cropsci2005.0246
16	Xia X C Reif J C Melchinger A E Frish M Hoising D A Beck D Pixley K Warburton M L 2004 Genetic diversity among CIMMYT maize inbredlines investigated with SSR marker: I. Lowland tropical maizeCrop Sci44(6)22302237 (in Chinese)
17	Xin J S 2004 DNA Finger Frint of MaizeHybridsBeijingChina Agricultural Sci&techPress194196 (in Chinese)
18	Yang X P Ma Q D 1994 Study on geneticdiversity of maize hybrids in ChinaJ MaizeSci2(3)2528 (in Chinese)
19	Yuan L X Fu J H Warbu M Li X H Zhang S H Liu X Z Peng Z B 2000 Comparisonof genetic diversity among maize inbred lines based on RFLPs, SSRs,AFLPs and RAPDsActa Genet Sin27(8)725733 (in Chinese)
20	Zeng S S 1990 The maize germplasm basis of hybrids in ChinaSci Agric Sin23(4)193 (in Chinese)

Viewed

Full text

Abstract

Cited

Shared

Discussed