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Frontiers of Agriculture in China

ISSN 1673-7334

ISSN 1673-744X(Online)

CN 11-5729/S

Front. Agric. China    2009, Vol. 3 Issue (4) : 397-401     DOI: 10.1007/s11703-009-0065-3
Research articles |
Cytological effects of different mutagens in soybean [ Glycine max (L.) Merrill]
Mudasir Hafiz KHAN,Sunil Dutt TYAGI,
Ph, D Scholar-cum-Research Associate, Department of Plant Breeding and Genetics, Kisan (P. G.) College, Simbhaoli, Ghaziabad, (U. P.), 245201, India;
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Abstract  The effect of gamma rays, ethyl methane sulphonate, and their three combinations on various cytological parameters in M1 generation was studied in two soybean cultivars, Pusa-16 and PK-1042. Combined treatments exhibited high meiotic abnormalities in Pusa-16 as compared with the individual treatments of ethyl methane sulphonate and gamma rays. In PK-1042 the highest frequency of meiotic abnormalities was exhibited by gamma rays, while in Pusa-16 the frequencies of univalents were high as compared with other meiotic abnormalities. Fragments/bridges, laggards, ring and rod bivalents were other types of meiotic abnormalities that were noticed in both the cultivars. The frequencies of irregular cells in the two cultivars were high in combined treatments followed by ethyl methane sulphonate and gamma rays. The highest percentage of pollen sterility was exhibited by the combined treatments and the lowest was exhibited by gamma rays. In Pusa-16, the increase in doses of gamma rays and ethyl methane sulphonate increased the pollen sterility percentage, while in the combined treatments, the increase was noticed up to the intermediate dose and then gradually decreased at higher doses. On the other hand, in PK-1042 the increase in ethyl methane sulphonate dose increased sterility percentage, while no definite trend was noticed in gamma rays and combined treatments.
Keywords Glycine max      mutagen      cytological abnormality      
Issue Date: 05 December 2009
URL:     OR
Arslan O, Bal S, Mirici S, Yenice N(2001). Meiotic studies in the M2 generationof Helianthus annuus L. varietyEkiz 1 after gamma irradiation. Helia, 24(35): 33―38
Bione N C P, Pagliarini M S, de Almeida L A(2002b). A newand distinctive male-sterile, female-fertile desynaptic mutant insoybean (Glycine max L.). Hereditas, 136(2): 97―103

doi: 10.1034/j.1601-5223.2002.1360202.x
Bione N C P, Pagliarini M S, de Almeida L A(2002a). An asynapticmutation in soybean [Glycine max (L.) Merrill] associated with total absence of sister chromatidcohesiveness. Cytologia, 67(2): 177―183

doi: 10.1508/cytologia.67.177
Bione N C P, Pagliarini M S, Toledo J F F(2000). Meioticbehavior of several Brazilian soybean varieties. Genet Mol Biol, 23(3): 623―631

doi: 10.1590/S1415-47572000000300022
Dixit P, Dubey D K(1986). Mutagenic efficiency of gamma rays, NMU and their combination inlentil (Lens culnaris Med.) VarietyT 36. Indian J Genet, 43(3): 501―506
Ekberg J(1969). Different types of sterility inducedin barley by ionizing radiations and chemical mutagens. Hereditas, 63: 257―278
Graybosch R A, Palmer R G(1988). Male sterility in soybean―an overview. Am J Bot, 75: 144―156

doi: 10.2307/2443913
Ignacimuthu S, Babu C R(1992). Induced variation in pod and seed traits of wild and cultivated beans. Journal of Nuclear and Agricultural Biology, 21(4): 286―292
Kumar S, Dubey D K(1998). Influence of separate and simultaneous applications of gamma rays,DES and EMS on meiosis in khesari (Lathyrussativus L.). J Genet Breed, 52(4): 295―300
Ladizinsky G, Newell C A, Hymowitz T(1979). Giemsastaining of soybean chromosomes. J Hered, 70: 415―416
Mansour K S(1994). Effects of gamma irradiation on mitosisof Lens esculenta, Trigonella foenum graecum and Vicia faba. EgyptianJ Bot, 34(1): 81―92
Newell C A, Hymowitz T(1980). Cytology of Glycine tabacina. J Heredity, 71: 175―178
Palmer R G(1976). Cytogenetics in soybean improvement. In: Proceedings of the Sixth Soybean Seed ResearchConference. Chicago: American Seed Trade Association, 56―66
Palmer R G, Albertsen M C, Horner H T, Skorupska H(1992). Male sterility in soybean and maize:developmental comparisons. Nucleus, 35: 1―18
Reddy V R K, Annadurai M(1992). Cytological effects of different mutagens in lentil(Lens culinaris Medik). Cytoiogia, 57: 213―16
Reddy V R K, Revathi R, Nalini R(1991). Effectof physical and chemical mutagens on the meiotic behaviour in barleyand wheat. J Indian Bot Soc, 70: 113―119
Sen N K, Vidyabhusan R V(1960). Tetraploid soybean. Euphytica, 9: 317―322
Singh R J, Hymowitz T(1988). The genomic relationship between Glycine max (L.) Merr. and G.soja Sieb. and Zucc. as revealed by pachytene chromosomeanalysis. Theor Appl Genet, 76: 705―711

doi: 10.1007/BF00303516
Vinita S, Kumar G, Kumar P(2004). Comparative mutagenicityof gamma rays and EMS in Cicer arietinum L.. J Cytol Genet, 5(1): 21―26
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