Evolutionary engineering of <italic>Phaffia rhodozyma</italic> for astaxanthin-overproducing strain

doi:10.1007/s11705-012-1276-3

Front Chem Sci Eng

2012, Vol. 6

Issue (2) : 174-178 https://doi.org/10.1007/s11705-012-1276-3

RESEARCH ARTICLE

Evolutionary engineering of Phaffia rhodozyma for astaxanthin-overproducing strain

Jixian GONG^1,2(

), Nan DUAN², Xueming ZHAO²

1. School of Textiles, Tianjin Polytechnic University, Tianjin 300160, China; 2. School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China

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Abstract

Evolutionary engineering is a novel whole-genome wide engineering strategy inspired by natural evolution for strain improvement. Astaxanthin has been widely used in cosmetics, pharmaceutical and health care food due to its capability of quenching active oxygen. Strain improvement of Phaffia rhodozyma, one of the main sources for natural astaxanthin, is of commercial interest for astaxanthin production. In this study a selection procedure was developed for adaptive evolution of P. rhodozyma strains under endogenetic selective pressure induced by additive in environmental niches. Six agents, which can induce active oxygen in cells, were added to the culture medium respectively to produce selective pressure in process of evolution. The initial strain, P. rhodozyma AS2-1557, was mutagenized to acquire the initial strain population, which was then cultivated for 550 h at selective pressure and the culture was transferred every 48h. Finally, six evolved strains were selected after 150 generations of evolution. The evolved strains produced up to 48.2% more astaxanthin than the initial strain. Our procedure may provide a promising alternative for improvement of high-production strain.

Keywords evolutionary engineering astaxanthin strain improvement

Corresponding Author(s): GONG Jixian,Email:gongjixian@126.com

Issue Date: 05 June 2012

Cite this article:

Jixian GONG,Nan DUAN,Xueming ZHAO. Evolutionary engineering of Phaffia rhodozyma for astaxanthin-overproducing strain[J]. Front Chem Sci Eng, 2012, 6(2): 174-178.

URL:

https://academic.hep.com.cn/fcse/EN/10.1007/s11705-012-1276-3
https://academic.hep.com.cn/fcse/EN/Y2012/V6/I2/174

Fig.1 Stress resistance of wild strain. The growth curve of in the culture with (a) ionone, (b) diphenylamine, (c) NaCl, (d) TiO, (e) HO, and (f) NaClO

Fig.2 Evolution of in cultures containing (a) ionone, (b) diphenylamine, (c) NaCl, (d) TiO, (e) HO, and (f) NaClO

Fig.3 Growth character of the evolved strains

Fig.4 Astaxanthin of evolved strain by cultured in medium with (1) ionone, (2) diphenylamine, (3) NaCl, (4) TiO, (5) HO, (6) NaClO, and (7) the wild strain.

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