Designer enzyme for green materials innovation: Lactate-polymerizing enzyme as a key catalyst

doi:10.1007/s11705-017-1636-0

Front. Chem. Sci. Eng.

2017, Vol. 11

Issue (1) : 139-142 https://doi.org/10.1007/s11705-017-1636-0

VIEWS & COMMENTS

Designer enzyme for green materials innovation: Lactate-polymerizing enzyme as a key catalyst

Seiichi Taguchi^1,²(

)

¹. Graduate School of Engineering, Hokkaido University, Hokkaido 060-0808, Japan
². JST, CREST, 5 Sanbancho, Chiyoda-ku, Tokyo 102-0075, Japan

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Abstract

Establishment of the regeneratable whole-cell catalyst platform for the?production of biobased polymeric materials is a?typical topic of synthetic biology. In this commentary, discovery story of a “lactate-polymerizing enzyme” (LPE)?and LPE-based?achievements for creating a new variety of polyesters with incorporated unnatural monomers are presented. Besides the importance of microbial platform itself is discussed referring to the “ballooning”-Escherichia coli.

Keywords synthetic biology enzyme evolutionary engineering polyhydroxyalkanoate

PACS:

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Corresponding Author(s): Seiichi Taguchi

Just Accepted Date: 24 January 2017 Issue Date: 17 March 2017

Cite this article:

Seiichi Taguchi. Designer enzyme for green materials innovation: Lactate-polymerizing enzyme as a key catalyst[J]. Front. Chem. Sci. Eng., 2017, 11(1): 139-142.

URL:

https://academic.hep.com.cn/fcse/EN/10.1007/s11705-017-1636-0
https://academic.hep.com.cn/fcse/EN/Y2017/V11/I1/139

Fig.1 Performances of artificially evolved enzymes.

Schematic illustration of LPE represented by designer enzymes is presented here. This figure shows the process conversion for LA-based polymers production from chemo-process to bio-process. LPE functions as an alternative of metal catalyst in the living cell. PCT: propiony-CoA transferase

Fig.2 Excellent transparency and improved flexibility of LA-based copolymer.

Solvent cast-film of P(31 mol-%-co-3HB) exhibits higher properties in terms of transparency and mechanical flexibility

Fig.3 Ballooning microbial factory.

Enhanced cell volume of Escherichia coli took place associated with polymer accumulation like a balloon. This morphological change was caused by gene disruption of MtgA which functions as a forming machinery of peptidoglycan

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