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Efficient production of D-1,2,4-butanetriol from D-xylose by engineered Escherichia coli whole-cell biocatalysts |
Shewei Hu1, Qian Gao1, Xin Wang1, Jianming Yang2, Nana Xu1, Kequan Chen1(), Sheng Xu1, Pingkai Ouyang1 |
1. State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing 211816, China 2. Xi’an Modern Chemistry Research Institute, Xi’an 710065, China |
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Abstract We have developed a whole-cell bioconversion system for the production of D-1,2,4-butanetriol (BT) from renewable biomass. A plasmid pETduet-xylB-yjhG-T7-adhP-T7-mdlC was constructed and transformed to Escherichia coli BL21(DE3) to obtain the whole cells of E. coli BL21-XYMA capable of bioconversion D-xylose to BT. Then, the factors including carbon sources, nitrogen sources, metal ions, and culture conditions (pH, temperature, IPTG) were identified, and their effects on the whole-cell activity for BT production were investigated. To obtain the highest whole-cell activity, the optimal cultivation parameters are: 15 g·L−1 yeast extract, 5 g·L−1 sucrose, 3 g·L−1 KH2PO4, 5 g·L−1 NaCl, 3 g·L−1 NH4Cl, 0.25 g·L−1 MgSO4∙7H2O and 1 mL·L−1 the mixture of trace elements. With the optimized whole cells of E. coli BL21-XYMA, 60 g·L−1 of xylose was converted to 28 g·L−1 BT with a molar yield of 66.0%, which is higher than those reported in the biotechnological system.
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Keywords
D-1,2,4-butanetriol
whole-cell bioconversion
carbon source
nitrogen sources
metal ions
culture conditions
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Corresponding Author(s):
Kequan Chen
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Just Accepted Date: 10 April 2018
Online First Date: 31 August 2018
Issue Date: 03 January 2019
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