Genomic regions associated with the sex-linked inhibitor of dermal melanin in Silkie chicken

doi:10.15302/J-FASE-2014018

Front. Agr. Sci. Eng.

2014, Vol. 1

Issue (3) : 242-249 https://doi.org/10.15302/J-FASE-2014018

RESEARCH ARTICLE

Genomic regions associated with the sex-linked inhibitor of dermal melanin in Silkie chicken

Ming TIAN,Rui HAO,Suyun FANG,Yanqiang WANG,Xiaorong GU,Chungang FENG,Xiaoxiang HU(

),Ning LI(

)

State Key Laboratory for Agrobiotechnology, China Agricultural University, Beijing 100193, China

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Abstract

A unique characteristic of the Silkie chicken is its fibromelanosis phenotype. The dermal layer of its skin, its connective tissue and shank dermis are hyperpigmented. This dermal hyperpigmentation phenotype is controlled by the sex-linked inhibitor of dermal melanin gene (ID) and the dominant fibromelanosis allele. This study attempted to confirm the genomic region associated with ID. By genotyping, ID was found to be closely linked to the region between GGA_rs16127903 and GGA_rs14685542 (8406919 bp) on chromosome Z, which contains ten functional genes. The expression of these genes was characterized in the embryo and 4 days after hatching and it was concluded that MTAP, encoding methylthioadenosinephosphorylase, would be the most likely candidate gene. Finally, target DNA capture and sequence analysis was performed, but no specific SNP(s) was found in the targeted region of the Silkie genome. Further work is necessary to identify the causal ID mutation located on chromosome Z.

Keywords sex-linked inhibitor of dermal melanin (Id) Silkie chromosome Z

Corresponding Author(s): Xiaoxiang HU,Ning LI

Online First Date: 03 December 2014 Issue Date: 27 January 2015

Cite this article:

Ming TIAN,Rui HAO,Suyun FANG, et al. Genomic regions associated with the sex-linked inhibitor of dermal melanin in Silkie chicken[J]. Front. Agr. Sci. Eng. , 2014, 1(3): 242-249.

URL:

https://academic.hep.com.cn/fase/EN/10.15302/J-FASE-2014018
https://academic.hep.com.cn/fase/EN/Y2014/V1/I3/242

Fig.1 Founding breeds for the mapping populations

Fig.2 Shank color of crosses (F₂ generation) at 12 weeks

Fig.3 The results of Q-PCR analysis of the expression of ten target functional genes. (a) ALDH7A1 mRNA; (b) CCDC112 mRNA; (c) CDKN2B mRNA; (d) FEM1C mRNA; (e) GRAMD3 mRNA; (f) MTAP mRNA; (g) PGGT1B mRNA; (h) TRIM36 mRNA; (i) ZNF608 mRNA; (j) β-4-GALT1 mRNA. BLH: White Leghorn. WJ: Silkie. * means P<0.05. ** means P<0.01. *** means P<0.001. These P values are compared between BLH and WJ in each time and tissue. E: embryo. 4D: the 4th day after born. HL: hind-leg. F: shank skin. S: skin.

Tab.1 Sequencing data based on genotype

Tab.2 Sequencing data without consideration of the genotype

Tab.3 InDel sequences

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[1]	Ming TIAN,Suyun FANG,Yanqiang WANG,Xiaorong GU,Chungang FENG,Rui HAO,Xiaoxiang HU,Ning LI. *Inverted duplication including Endothelin 3* closely related to dermal hyperpigmentation in Silkie chickens**[J]. Front. Agr. Sci. Eng. , 2014, 1(2): 121-129.

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